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Heterotrophy denitrifying bacteria for synchronously degrading sulfide, nitrate and organic carbon source

A denitrifying bacteria and nitrate technology, applied in chemical instruments and methods, microbial-based methods, biochemical equipment and methods, etc., can solve problems such as poor water shock resistance, large footprint, and long growth cycle. Achieve the effect of good water shock resistance, small footprint and short growth cycle

Active Publication Date: 2012-05-09
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high operating cost, large floor area, high one-time investment and poor treatment effect in the traditional method of treating sulfur-containing and nitrogen-containing organic wastewater; while the use of autotrophic denitrifying bacteria has a growth cycle of bacteria long, poor resistance to hydraulic shock and low treatment capacity, and provide a heterotrophic denitrifier that simultaneously degrades sulfide, nitrate and organic carbon sources

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  • Heterotrophy denitrifying bacteria for synchronously degrading sulfide, nitrate and organic carbon source
  • Heterotrophy denitrifying bacteria for synchronously degrading sulfide, nitrate and organic carbon source
  • Heterotrophy denitrifying bacteria for synchronously degrading sulfide, nitrate and organic carbon source

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specific Embodiment approach 1

[0012] Specific implementation mode 1: In this implementation mode, the heterotrophic denitrifying bacteria C27, which simultaneously degrades sulfide, nitrate and organic carbon sources, has been identified as a new species of Pseudomonas sp. Committee General Microbiology Center (CGMCC), the preservation address is the Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, the preservation date is January 26, 2010, and the preservation number is CGMCC No.3612; simultaneous degradation of sulfide, Heterotrophic denitrifying bacteria of nitrate and organic carbon source are bacilli, 0.5-1.0 μm in width, 1.0-2.0 μm in length, Gram staining is negative, no spores, with unipolar flagella, able to move, growth temperature range Heterotrophic denitrifying bacteria that simultaneously degrade sulfide, nitrate and organic carbon sources grow on the culture medium. The colony is light yellow, round, without protrusions, regular edge...

specific Embodiment approach 2

[0016] Specific embodiment two: this embodiment synchronously degrades the heterotrophic denitrifying bacteria of sulfide, nitrate and organic carbon source and obtains from the granular sludge in the synchronous desulfurization and denitrification EGSB reactor; Screening is carried out according to the following steps: 1. 1. Collect the granular sludge in the synchronous desulfurization and denitrification EGSB reactor, dilute it with sterile water after ultrasonic crushing, and obtain a concentration of 10 1 ~10 9 Bacterial suspension per mL, then inoculate the bacterial suspension in the liquid medium according to the volume ratio of 1:10~20, enrich and cultivate for 48 hours; The bacterial suspension was inoculated on the solid separation medium, and cultured at 25-35°C for 24 hours to obtain a single colony; 3. Pick a single colony and transfer it to a liquid medium, and enrich it at 25-35°C Collect and cultivate until the medium becomes turbid; 4. Pick the turbid bacter...

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Abstract

The invention discloses heterotrophy denitrifying bacteria for synchronously degrading sulfide, nitrate and an organic carbon source, relating to heterotrophy denitrifying bacteria and solving the problems of high operation cost, large floor area, high once investment and poor treatment result of the traditional method for treating organic waste water containing sulfur and nitrogen, as well as the problems of long growth cycle, poor water impact resistance and low treatment capability generated when autotrophy denitrifying bacteria is adopted. The heterotrophy denitrifying bacteria for synchronously degrading the sulfide, the nitrate and the organic carbon source is a new variety of pseudomonas and is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation address of Institute of Microbiology of Chinese Academy of Sciences (IMCAS) and the preservation number of CGMCC No.3612 on January 26th, 2010. The bacteria of the invention can be used fortreating the sulfide, the nitrate and the organic carbon source synchronously, therefore, effects of reduced cost, small floor area, low investment, good treating effect, short bacteria growth cycle and good water impact resistance can be achieved.

Description

technical field [0001] The invention relates to a heterotrophic denitrifying bacteria. Background technique [0002] With the rapid development of petrochemical, pharmaceutical, food fermentation, papermaking and other industries, the pollution of organic wastewater containing sulfur and nitrogen is becoming more and more serious. Compared with physical and chemical treatment, biological wastewater treatment has the advantages of high efficiency, low operating cost, and no toxic by-products, so it is favored. For the biological treatment of sulfur- and nitrogen-containing organic wastewater, most of these pollutants are removed by desulfurization and denitrification in two separate reaction systems, that is, sulfide-containing pollutants are passed through the biological process of sulfide-oxidizing bacteria. Oxidation, oxidize it to elemental sulfur to achieve the removal of sulfide; and nitrogenous wastewater containing nitrate, through the biological metabolism of denitr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 任南琪陈川王爱杰何国领张莉徐熙俊周旭刘充高灵芳许继飞李笃中
Owner HARBIN INST OF TECH
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