Method for preparing natural-detergent feed solution by using tea saponin fluid

A technology of tea saponin and raw material solution, applied in the direction of microorganism-based method, preparation of detergent mixture composition, detergent composition, etc., can solve the problem of poor effect of protein stained cloth and carbon black stained cloth, etc. problems, to achieve the effect of improving decontamination ability, saving resources and improving decontamination effect

Inactive Publication Date: 2011-04-13
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After research, it is found that tea saponin has a good decontamination effect on sebum-stained cloth, but it has poor effect on protein-stained cloth and carbon black-stained cloth. Therefore, tea saponin alone is not effective as a detergent and must be compatible with other surfactants , in order to obtain a more ideal decontamination effect
[0006] Some researcher matches tea saponin with other additives to improve the decontamination effect of tea saponin, such as Li Yuntao, Jia Bin in "The Extraction Technology of Tea Saponin and Its Application in Detergent" (Tea Science, 2006, 26 (3): 199~203) In the article, tea saponin and sodium perborate are compounded to prepare a good performance detergent additive, but a large amount of other surfactants are still used in the formula

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Fermentation of tea saponin solution by Bacillus licheniformis AS 1.807.

[0030] The tea saponin aqueous solution is sterilized, and after being inoculated with microbial strains, it is cultivated at 36° C. for 48 hours with stirring.

[0031] Centrifuge the fermented liquid with a centrifuge first, and then filter it with a 0.1-0.6 μm membrane to obtain a tea saponin solution containing enzymes such as protease and amylase. About 18u / mL of lipase, this solution is used as the basic raw material of detergent. Determination of protease activity adopts Folin-phenol reagent method: Folin-phenol reagent can be reduced by phenolic compounds to turn blue (mixture of molybdenum blue and tungsten blue) under alkaline conditions, because there are amino acids containing phenolic groups in protein molecules ( Such as tyrosine, tryptophan, etc.), can make protein and its hydrolyzate exhibit the above reaction. Therefore, this principle can be used to measure protease activity. ...

Embodiment 2

[0033] Fermentation of tea saponin solution by Candida AS2.625.

[0034] Sterilize the tea saponin aqueous solution, inoculate the microbial strains, and cultivate for 72 hours at 28°C. And with stirring.

[0035] Centrifuge the fermentation liquid with a centrifuge first, and then filter it with a 0.1-0.4 μm membrane to obtain a tea saponin solution containing enzymes such as protease, in which the protease activity is about 620u / mL, the α-amylase is about 230u / mL, and the fat Enzyme 18u / mL or so, this solution is used as the basic raw material of detergent.

Embodiment 3

[0037] Fermentation with Bacillus natto AS 1.1086.

[0038] Sterilize the tea saponin aqueous solution, inoculate the microbial strains, and cultivate at 37° C. for 48 hours. And with stirring.

[0039] Centrifuge the fermentation liquid with a centrifuge first, and then filter it with a 0.1-0.6 μm membrane to obtain a tea saponin solution containing protease and other enzyme systems, in which the protease activity is about 300u / mL, the α-amylase is about 8u / mL, and the fat Enzyme 10u / mL or so, this solution is used as the basic raw material of detergent.

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Abstract

The invention relates to a method for preparing a natural-detergent feed solution by using a tea saponin fluid, which overcomes the defect that the dirt-removing power of the existing tea saponin fluid is not strong. The method is implemented through the step of carrying out fermentation on the tea saponin fluid by microbes so as to obtain a feed solution for preparing detergents, wherein the microbes are germs, saccharomycetes and mycetes which can grow in a tea saponin solution and can produce lipases, proteases and amylase in the process of fermentation. The method provided by the invention has the advantages that because the tea saponin fluid is fermented by the microbes which can grow in a tea saponin environment so as to produce a series of enzymatic systems having dirt-removing effect, therefore, the dirt-removing effect and dirt-removing range of the tea saponin fluid are remarkably improved, meanwhile, the components without dirt-removing power can be consumed in the process of fermentation, and the dirt-removing effect of the tea saponin is also correspondingly improved.

Description

technical field [0001] The invention relates to a method for producing washing liquid raw material liquid by using tea saponin liquid. Background technique [0002] Camellia oleifera is a unique oil plant in my country. In addition to rich triglycerides, its seeds also contain a large amount of water-soluble nutrients, including tea saponin, protein, carbohydrates, phospholipids, etc. The aqueous solution obtained by extracting tea seed oil by water enzymatic method and water substitution method, or the aqueous solution extracted by pressing and leaching tea seed cake with water, because the main components are tea saponin and water-soluble sugar, so this The aqueous solution can be called tea saponin liquid, [0003] At present, the washing products widely used in our country are chemical detergents compounded with various chemical additives and water. Because the chemical components contained in it penetrate into human blood through the skin, it is harmful to human health...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D11/00C11D7/42C12N1/20C12N1/14C12N1/16C12N9/16C12N9/50C12N9/26C12R1/10C12R1/085C12R1/685C12R1/69C12R1/80C12R1/72C12R1/73
Inventor 周建平郭华周玥周传云
Owner HUNAN AGRICULTURAL UNIV
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