Method for preparing natural-detergent feed solution by using tea saponin fluid
A technology of tea saponin and raw material solution, applied in the direction of microorganism-based method, preparation of detergent mixture composition, detergent composition, etc., can solve the problem of poor effect of protein stained cloth and carbon black stained cloth, etc. problems, to achieve the effect of improving decontamination ability, saving resources and improving decontamination effect
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Embodiment 1
[0029] Fermentation of tea saponin solution by Bacillus licheniformis AS 1.807.
[0030] The tea saponin aqueous solution is sterilized, and after being inoculated with microbial strains, it is cultivated at 36° C. for 48 hours with stirring.
[0031] Centrifuge the fermented liquid with a centrifuge first, and then filter it with a 0.1-0.6 μm membrane to obtain a tea saponin solution containing enzymes such as protease and amylase. About 18u / mL of lipase, this solution is used as the basic raw material of detergent. Determination of protease activity adopts Folin-phenol reagent method: Folin-phenol reagent can be reduced by phenolic compounds to turn blue (mixture of molybdenum blue and tungsten blue) under alkaline conditions, because there are amino acids containing phenolic groups in protein molecules ( Such as tyrosine, tryptophan, etc.), can make protein and its hydrolyzate exhibit the above reaction. Therefore, this principle can be used to measure protease activity. ...
Embodiment 2
[0033] Fermentation of tea saponin solution by Candida AS2.625.
[0034] Sterilize the tea saponin aqueous solution, inoculate the microbial strains, and cultivate for 72 hours at 28°C. And with stirring.
[0035] Centrifuge the fermentation liquid with a centrifuge first, and then filter it with a 0.1-0.4 μm membrane to obtain a tea saponin solution containing enzymes such as protease, in which the protease activity is about 620u / mL, the α-amylase is about 230u / mL, and the fat Enzyme 18u / mL or so, this solution is used as the basic raw material of detergent.
Embodiment 3
[0037] Fermentation with Bacillus natto AS 1.1086.
[0038] Sterilize the tea saponin aqueous solution, inoculate the microbial strains, and cultivate at 37° C. for 48 hours. And with stirring.
[0039] Centrifuge the fermentation liquid with a centrifuge first, and then filter it with a 0.1-0.6 μm membrane to obtain a tea saponin solution containing protease and other enzyme systems, in which the protease activity is about 300u / mL, the α-amylase is about 8u / mL, and the fat Enzyme 10u / mL or so, this solution is used as the basic raw material of detergent.
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