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Energy-saving method for culturing haliotis diversicolor aquatilis

A nine-hole abalone, energy-saving technology, used in microorganism-based methods, biochemical equipment and methods, climate change adaptation, etc. Residue and other problems, to achieve the effect of improving immunity, improving intestinal environment, and accelerating growth rate

Inactive Publication Date: 2013-06-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term use of antibiotics, especially the abuse of antibiotics, will also lead to the imbalance of normal flora in the gastrointestinal tract of abalone, which will have a negative impact on the immunity of the body of abalone, and cause side effects such as drug resistance and drug residues, which will cause serious adverse effects on the aquaculture industry and consumers. of human health have posed potential hazards
In addition, some Chinese herbal medicines are used for prevention and treatment, but there is a shortcoming of slow effect.

Method used

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  • Energy-saving method for culturing haliotis diversicolor aquatilis
  • Energy-saving method for culturing haliotis diversicolor aquatilis
  • Energy-saving method for culturing haliotis diversicolor aquatilis

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Preparation of the Bdellovibrio Bruldoid Bacteria Liquid for Haliotis diversicolor aquatilis Seedling Cultivation

[0031] Inoculate 0.5mL of 10 in a conical flask containing 100mL of nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) 7cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, strain number: GIM 1.172, sourced from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 35°C shaker culture for 12 hours, the culture solution was centrifuged at 4°C, 5000rpm for 15min , discard the supernatant. Suspend and precipitate the bacterial cells with 5mL normal saline, and then add them to a liquid culture medium containing 100mL DNB (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, dissolve in 1000mL distilled water, pH 7.2 ~ 7.6) Erlenmeyer flask, add 1mL containing 10 3 Bdellovibrio BDFM05 in pfu / mL (CCTCC M209172). Incubate on a constant temperature shaker at 250 rpm at 28°C fo...

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Abstract

The invention discloses an energy-saving method for culturing haliotis diversicolor aquatilis. The method comprises the following steps of: (1) culturing algae; (2) adding nutrient salt; (3) collecting the spats and distributing eggs, namely, changing water in an algae culturing pool, adding bdellovibrio bacteriovorus plasmid bacterial liquid into the pool until the concentration of bdellovibrio bacteriovorus plasmid in the pool water is between 10 and 107 pfu / mL and distributing 40,000 to 50,000 haliotis diversicolor aquatilis fertilized eggs in each pool during spat distribution; (4) changing water, namely, changing the pool water once every 5 to 30 days and adding the bdellovibrio bacteriovorus plasmid bacterial liquid into the pool water after the water is changed each time until the concentration of the bdellovibrio bacteriovorus plasmid in the pool water is between 10 and 107 pfu / mL; and (5) stripping and collecting. The spat culturing method remarkably promotes the growth of the haliotis diversicolor aquatilis, greatly increases the survival rate of the haliotis diversicolor aquatilis and improves the water quality of a culturing environment. The bdellovibrio bacteriovorus plasmid does not have toxic or side effect on abalone, so that the method is suitable for large-scale culturing of the abalone and provides new culturing technology for promoting the spat culturing and growth of haliotis diversicolor aquatilis.

Description

technical field [0001] The invention belongs to the field of aquaculture shellfish seedling raising methods, in particular to a novel, water-saving, energy-saving, green and environment-friendly seedling raising method capable of promoting the growth of haliotis diversicolor aquatilis abalone seedlings. Background technique [0002] Nine-hole abalone (also known as variegated abalone) is one of the important economic species of Abalone in the southern sea area of ​​my country. Due to the advantages of fast growth and relatively low requirements for breeding conditions, this kind of abalone has now developed into Taiwan and other countries. The most important cultured species of Babaceae in the southeast coast of the mainland. However, since 2000, a large number of artificially cultivated abalone seedlings have died from Fujian to the coast of Hainan, and more than 90% of the abalone seedling farms failed to cultivate abalone seedlings, forcing many farmers to give up breeding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K10/30A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏陈小红
Owner SOUTH CHINA UNIV OF TECH
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