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Water-saving haliotis diversicolor aquatilis cultivation method

A breeding method, the technology of the nine-hole abalone, applied in the new, green and environment-friendly breeding technology, and energy-saving fields, can solve the problems of large pollution in sea areas, occurrence of diseases, and imbalance of normal flora, so as to improve the intestinal environment, improve immunity, The effect of improving the survival rate

Inactive Publication Date: 2013-09-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During the breeding process of abalone, the use of antibiotics has played a positive role in inhibiting the reproduction of pathogenic microorganisms and reducing the poisonous effect of their metabolic toxins on abalone. However, the long-term use or abuse of antibiotics has gradually revealed its various disadvantages.
For example, the imbalance of the normal flora in the gastrointestinal tract of abalone will have adverse effects on the immunity of the body of the abalone, resulting in side effects such as drug resistance and drug residues, which will bring potential harm to the healthy development of the aquaculture industry and human health.
In addition, in the current industrialized land-based abalone farming in my country, the main method is to extract seawater from the outside and enter the culture pond after sand filtration and sedimentation, or to extract sand well water for running water culture, and the waste water is directly discharged into the sea area through the pipeline. This direct pumping and direct discharge method It has the disadvantages of high energy consumption and great pollution to sea areas, which not only increases the cost of breeding, but also becomes a major hidden danger of diseases in the future

Method used

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Embodiment 1

[0030] (1) Preparation of Bdellovibrio Swimming Body Bacterial Liquid

[0031] Bdellovibrio swimmers were fermented by the fermentation method of high-density Bdellovibrio swimmers: in a medium containing 100mL nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) Inoculate 0.5mL of 10 7cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, strain number: GIM 1.172, sourced from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 28°C shaker culture for 18 hours, the culture solution was centrifuged at 4°C, 5000rpm 15min, discard the supernatant. Suspend and precipitate the bacteria with 5mL of normal saline, and then add it to a 100mL DNB (dilute nutrientbroth) liquid medium (0.8g of nutrient broth, 0.5g of casein acid hydrolyzate, 0.1g of yeast extract, dissolved in 1000mL of distilled water In the Erlenmeyer flask with a pH value of 7.2~7.6), add 1mL containing 10 3 Bdellovibrio BDFM05 in pfu / mL (CCTCC M209172)....

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Abstract

The invention discloses a water-saving haliotis diversicolor aquatilis cultivation method which comprises the following steps: taking unpolluted deep sea water in the marine space as the cultivation water; selecting healthy and good-conditioned haliotis diversicolor aquatilis fingerlings and putting them into the cultivation pool; pouring bdellovibrio swimming body?bacterial liquid into the cultivation pool till the concentration of the bdellovibrio swimming body reaches 10-10<7> pfu / ml; feeding once a day with the amount of bait about 5% to 15% of the weight of the haliotis diversicolor aquatilis; soaking the bait in the bdellovibrio swimming body bacterial liquid with a concentration of 10-10<7> pfu / ml; and changing the pool water once every 5 to 30 days and sprayingm the bdellovibrio swimming body ?bacterial liquid after each water change. The cultivation method provided by this invention noticeably facilitates the growth, immunity and survival rate of haliotis diversicolor aquatilis and improves the water quality of the cultivation environment. Due to no toxic or side effect of the bdellovibrio swimming body bacterial liquid to abalone, the bdellovibrio swimming body bacterial liquid can be applied to large-scale industrialized abalone cultivation.

Description

technical field [0001] The invention belongs to the technical field of seawater shellfish culture, and in particular relates to a novel, energy-saving, green and environment-friendly culture technology capable of promoting the growth of Haliotis diversicolor aquatilis. Background technique [0002] Jiukong abalone, also known as variegated abalone or Taiwan abalone, is delicious and nutritious. It is a rare seafood. It inhabits the rocky sea area with smooth tide, abundant seaweed, high transparency and high salinity. It is a nocturnal soft body. animal. In 1979, after the successful artificial breeding of Taiwan's Abalone, it began to breed in the northeast corner, and then spread to other areas of the island. The breeding method has developed from intertidal cofferdams to land plane and three-dimensional cages. In the early 1990s, Dongshan County, Fujian Province began to introduce Taiwan's nine-hole abalone breeding technology, and quickly spread it southward to the coas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏陈小红
Owner SOUTH CHINA UNIV OF TECH
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