Water-saving haliotis diversicolor aquatilis cultivation method
A breeding method, the technology of the nine-hole abalone, applied in the new, green and environment-friendly breeding technology, and energy-saving fields, can solve the problems of large pollution in sea areas, occurrence of diseases, and imbalance of normal flora, so as to improve the intestinal environment, improve immunity, The effect of improving the survival rate
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[0030] (1) Preparation of Bdellovibrio Swimming Body Bacterial Liquid
[0031] Bdellovibrio swimmers were fermented by the fermentation method of high-density Bdellovibrio swimmers: in a medium containing 100mL nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) Inoculate 0.5mL of 10 7cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, strain number: GIM 1.172, sourced from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 28°C shaker culture for 18 hours, the culture solution was centrifuged at 4°C, 5000rpm 15min, discard the supernatant. Suspend and precipitate the bacteria with 5mL of normal saline, and then add it to a 100mL DNB (dilute nutrientbroth) liquid medium (0.8g of nutrient broth, 0.5g of casein acid hydrolyzate, 0.1g of yeast extract, dissolved in 1000mL of distilled water In the Erlenmeyer flask with a pH value of 7.2~7.6), add 1mL containing 10 3 Bdellovibrio BDFM05 in pfu / mL (CCTCC M209172)....
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