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F-gene type attenuated live mumps vaccine and preparation method and application thereof

A technology for attenuated live vaccines and mumps, which can be used in inactivation/attenuation, antiviral agents, pharmaceutical formulations, etc., and can solve problems such as allergic reactions

Inactive Publication Date: 2011-04-20
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Secondly, the currently routinely used attenuated live vaccines of the S79 strain and the Jeryl-Lynn strain are prepared on chicken embryo cells, and chicken embryo cells, as a heterogeneous cell matrix, may cause such diseases as allergic reactions and reversal of infectious diseases in poultry. Side effects ranging from severe to severe

Method used

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  • F-gene type attenuated live mumps vaccine and preparation method and application thereof
  • F-gene type attenuated live mumps vaccine and preparation method and application thereof
  • F-gene type attenuated live mumps vaccine and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] ——Cultivation and harvesting of virus stocks

[0028] The working seeds of the 24th generation were inoculated with mumps vaccine virus and diluted in serum-free cell maintenance solution at 0.1-0.25 moi, and directly inoculated into monolayer KMB17 cells without adsorption. Culture at 37°C for 7 to 9 days, and collect the harvested liquid after the cells are completely damaged. The harvested virus stock solution was centrifuged at 5000 rpm for 30 min to remove cell debris.

Embodiment 2

[0030] ——Preparation of semi-finished product of live attenuated mumps vaccine

[0031] Use MEM nutrient solution to dilute the virus stock solution to 5.0 lgCCID50 / ml, add an equal amount of lyoprotectant, and it is a semi-finished product. The formula of lyoprotectant is (2×): 8% sucrose, 8% trehalose, 2% glycine, 2% dextran, 1% human albumin, 0.2% arginine, 1% sodium glutamate

Embodiment 3

[0033] ——Detection of live attenuated mumps vaccine virus stock solution and semi-finished products

[0034] (1) The virus stock solution is a clear liquid without foreign matter and precipitation. The sterility test and mycoplasma test should be negative, without abnormal toxins, and the virus titer must be greater than 5.0 lgCCID50 / ml (see Table-1).

[0035] Table-1: Test results of virus stock solution

[0036]

[0037] (2) The sterility test of semi-finished products should be negative.

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Abstract

The invention discloses an F-gene type attenuated live mumps vaccine and a preparation method and application thereof, relating to an attenuated live mumps vaccine which is prepared in such a way that a separated F-gene type mumps attenuated live seed is inoculated to the human diploid cells (KMB17 strain) and is subjected to subsequent processes and the freezing-drying technology. The vaccine product has good immunogenicity and safety. The invention provides a cryoprotectant which does not contain glutin, thereby ensuring that the attenuated live mumps vaccine has good appearance, low moisture content and good stability.

Description

technical field [0001] The present invention relates to a mumps attenuated live vaccine, more specifically, the present invention relates to inoculating the isolated F genotype mumps attenuated virus into human diploid cells (KMB17 strain) and performing subsequent processing and freezing Dry technique, preparation of live attenuated mumps vaccine. Background technique [0002] Mumps virus (MuV) is the pathogen that causes mumps. According to the differences of nucleotides on the SH antigen, mumps virus can be divided into 12 genotypes, A-L. With the deepening of the molecular epidemiological analysis on mumps epidemic strains in China in recent years, increasing data all show that the current mumps virus strains prevalent in my country are mainly of the F genotype; at the same time, we mainly use The live attenuated vaccine is the S79 strain bred from the internationally accepted Jeryl-Lynn strain, and its gene is the A genotype popular abroad. More and more researchers h...

Claims

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Application Information

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IPC IPC(8): A61K39/155C12N7/04A61P31/14
Inventor 李琦涵梁燕马绍辉谢忠平刘龙丁崔平芳王丽春史长军董承红
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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