Catalase catalyst analog as well as preparation method and application thereof

A catalase and oxidase technology, applied in the field of multiferromagnetic materials, can solve the problems of laborious, unstable, difficult to recycle, etc.

Inactive Publication Date: 2012-06-13
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The task of the present invention is to provide a catalase-like catalyst, which has a very simple and reliable preparation process, cheap and easy-to-obtain raw materials, stable chemical activity, magnetic recovery, and can be used in aqueous solution, room temperature, near neutrality and without introducing any special Effective activation of H under the condition of external energy (referring to ultrasonic, microwave heating, strong light irradiation, etc.) 2 o 2 Produce strong oxidizing hydroxyl radicals and other characteristics to overcome the laborious, time-consuming, and costly nature of the prior art in the synthesis of catalase-like catalysts and the inability of natural catalase or existing hydrogen peroxide-like catalysts during use Stable, difficult to recycle and other deficiencies

Method used

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  • Catalase catalyst analog as well as preparation method and application thereof
  • Catalase catalyst analog as well as preparation method and application thereof
  • Catalase catalyst analog as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: use BiFeO of the present invention 3 Catalase-like catalysts activate H 2 o 2 To oxidize benzoic acid for indirect determination of H 2 o 2 Content

[0029] The prepared 5mg BiFeO 3 Add the powdery solid to 10mL distilled water, ultrasonically disperse for 1min, and add an equal volume of 1.0mmol L -1 Aqueous solution of benzoic acid was mixed, and H was added 2 o 2 (final concentration is 2.0×10 -8 ~2.0×10 -5 mol L -1 ). At this time, the substrate benzoic acid will be catalyzed and oxidized to generate a strong fluorescent substance, and the maximum emission wavelength of the fluorescent product is 405nm. After reacting for 15 minutes, quickly take 2 mL of the reaction solution and centrifuge, take out the centrifuged supernatant, and measure the fluorescence intensity of the supernatant at 405 nm on a fluorescence spectrophotometer. This BiFeO 3 The change value of the fluorescence intensity of catalase-like catalytic system at 405nm and H ...

Embodiment 2

[0030] Embodiment 2: use BiFeO of the present invention 3 Catalase-like catalysts activate H 2 o 2 To oxidize coumarin for indirect determination of H 2 o 2 Content

[0031] The prepared 5mg BiFeO 3 Add the powdery solid into 10mL distilled water, ultrasonically disperse for 1min, and add an equal volume of 0.5mmol L -1 Mix the coumarin aqueous solution, then add H2 o 2 (final concentration is 6.0×10 -8 ~5.0×10 -5 mol L -1 ). At this time, the substrate coumarin will be catalyzed and oxidized to generate a strong fluorescent substance 7-hydroxycoumarin, and the maximum emission wavelength of the fluorescent product is 456nm. After reacting for 10 min, quickly take 2 mL of the reaction solution and centrifuge, take out the centrifuged supernatant, and measure the fluorescence intensity of the supernatant at 456 nm on a fluorescence spectrophotometer. This BiFeO 3 The change value of the fluorescence intensity of catalase-like catalytic system at 456nm and H 2 o 2 ...

Embodiment 3

[0032] Embodiment 3: with BiFeO 3 Catalase-like catalysts activate H 2 o 2 to oxidize 3,3,5,5-tetramethylbenzidine for indirect determination of H 2 o 2 Content

[0033] The prepared 5mg BiFeO 3 Add the powdery solid into 5mL distilled water, ultrasonically disperse for 1min, and mix with an equal volume of 1mmol L -1 3,3,5,5-Tetramethylbenzidine aqueous solution mixed, then add H 2 o 2 (final concentration is 5.0×10 -7 ~2.0×10 -4 mol L -1 ). At this time, the substrate 3,3,5,5-tetramethylbenzidine will be catalyzed and oxidized to generate a blue product, and the maximum absorption wavelength of the blue product is 650 nm. After reacting for 15 minutes, quickly take 2 mL of the reaction solution and centrifuge, take out the centrifuged supernatant, and measure the absorbance of the supernatant at 650 nm on a UV-visible spectrophotometer. This BiFeO 3 The change value of the absorbance at 650nm of catalase-like catalytic system and H 2 o 2 Concentration at 5.0×1...

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Abstract

The invention provides a catalase catalyst analog which is bismuth ferrite. The preparation method comprises the following steps of: dissolving raw materials of ferric nitrate and bismuth nitrate in ethylene glycol monomethyl ether; adding a complexant and a dispersant to prepare sol and further obtain gel; calcining the obtained gel at high temperature; and postprocessing the obtained gel to obtain BiFeO3 catalase catalyst analog. The catalase catalyst analog has simple preparation process, low cost and stable chemical activity. Because the catalase catalyst analog can be subjected to magnetic recovery, the catalase catalyst can be reused many times, thereby overcoming the defects that the traditional catalase catalyst analog is synthesized in a mode wastes time, labor and money, is unstable and is hard to recover during application and the like. The catalase catalyst analog can serve as the substitute product of the natural catalase, can be used for detecting and analyzing H2O2 content in food samples, biological samples and environment samples and can be used for degrading and mineralizing organic pollutants.

Description

technical field [0001] The invention relates to the field of enzyme-like catalysis, in particular to multiferromagnetic material bismuth ferrite (BiFeO 3 ) has catalase-like catalytic activity, it can effectively activate hydrogen peroxide (H 2 o 2 ) to generate strong oxidizing hydroxyl radicals, thereby degrading and mineralizing organic pollutants, which can be applied to the detection of hydrogen peroxide content and water pollution treatment. technical background [0002] Environmental pollution, especially water pollution, is one of the focal issues restricting my country's economic development. Microbiological method is the most common sewage treatment method, but because many industrial wastewater contains a large amount of highly toxic and refractory organic pollutants, traditional microbial methods will cause microorganisms to fail to grow normally or even die. In recent years, people have tried to use titanium dioxide photocatalytic technology to treat industri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J23/843G01N21/64G01N21/33A62D3/38C02F1/72C02F101/30A62D101/04A62D101/20
Inventor 朱丽华罗玮唐和清
Owner HUAZHONG UNIV OF SCI & TECH
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