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Method for preparing Galium verum general flavone

A technology of total flavonoids and pomegranates, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve the problems of not being suitable for industrial production, low resin adsorption capacity, and large resin pollution, so as to reduce decomposition , Enhanced adsorption capacity, and reduced adsorption interference

Inactive Publication Date: 2011-05-04
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on fenugreek is currently limited to the study of pharmacology and chemical components, and there are few studies on the extraction process. The "macroporous adsorption resin separation and purification of diosmin" published by Lu Weihong et al. uses 70% ethanol for reflux extraction, and concentrates the extract to obtain the extract , dissolved in water on a macroporous adsorption resin column, this method extracts many impurities, the resin adsorption capacity is very low, and the resin is polluted greatly, so it is not suitable for industrial production
The method of extracting and preparing high-content total flavonoids from Potassium japonica has not yet been published in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Purify, dry, and pulverize the above-ground part of the procured fennel to obtain vegetal powder; weigh 5kg of vegetal powder and heat it to 70°C with 15L of water, extract 3 times for 2 hours each time, filter, and retain the filtrate. Add 30L of 50% ethanol aqueous solution to the filter residue for ultrasonic extraction for half an hour, combine all the filtrates, heat to 60°C, adjust the pH to 11 with 0.5% ammonia solution under stirring, keep at 60°C for 10 minutes, and filter while hot to obtain the filtrate, the filtrate Under the condition of 60°C, add 10% hydrochloric acid aqueous solution while stirring, adjust the pH to 3, cool to room temperature, let stand to precipitate the precipitate, filter with suction, collect the precipitate, add deionized water to disperse the precipitate, and use petroleum ether (boiling range 60°C) until the color of the petroleum ether extraction layer becomes lighter, the amount of petroleum ether used each time is 1 times the vo...

Embodiment 2

[0019] Purify, dry, and pulverize the above-ground part of the procured fennel to obtain vegetal powder; weigh 5kg of vegetal powder and heat it to 80°C with 23L of water, extract twice for 3 hours each time, filter, and retain the filtrate. Add 37L of 40% ethanol aqueous solution to the filter residue for ultrasonic extraction for 1 hour, combine all the filtrates, heat to 50°C, adjust the pH to 13 with 0.3% aqueous sodium hydroxide solution under stirring, keep at 50°C for 20 minutes, and filter while hot to obtain the filtrate , the filtrate is at 50°C, add 5% hydrochloric acid aqueous solution while stirring, adjust the pH to 2, cool to room temperature, let stand to separate out the precipitate, filter with suction, collect the precipitate, add deionized water to disperse the precipitate, and use petroleum ether ( Boiling range 60°C) until the color of the petroleum ether extraction layer becomes lighter, the amount of petroleum ether used each time is 0.5 times the volume...

Embodiment 3

[0021] Purify, dry, and pulverize the purchased ground part of lotus seed vegetable to obtain the powder of the lotus seed vegetable; weigh 5kg of the lotus seed vegetable powder and heat it to 70°C with 20L of water, extract 3 times, 1 hour each time, filter, retain the filtrate, and filter residue Then add 43L of 30% ethanol aqueous solution for ultrasonic extraction for 1 hour, combine all the filtrates, heat to 80°C, adjust the pH to 8.5 with 2% ammonia solution under stirring, keep at 80°C for 15 minutes, and filter while hot to obtain the filtrate. Under the condition of 80°C, add 10% sulfuric acid aqueous solution while stirring, adjust the pH to 5, cool to room temperature, let stand to precipitate precipitate, filter with suction, collect the precipitate, add deionized water to disperse the precipitate, and use petroleum ether (boiling range 60 ℃) until the color of the petroleum ether extraction layer becomes lighter, the amount of petroleum ether used each time is 1....

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PUM

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Abstract

The invention discloses a Method for preparing Galium verum general flavone, belonging to the technical field of plant extraction. The Galium verum general flavone mainly contains rutin, Palustroside, Diosmin, Diosmetin (2->1) glucose arabinose, isoquercitrin, Diosmetin 7-O-beta-D ascorbyl glucoside and the like. The general flavone in the Galium verum is obtained by being extracted by water, extracted by organic solvent, condensed, decontaminated and adsorbed and eluted by macroporous resin. The prepared Galium verum general flavone contains 50-95% of Galium verum. In the method, petroleum ether is adopted to extract and remove fat soluble impurities; column-loading buffer effectively removes impurities, resin adsorption interference can be lightened, and thus resin has strong adsorption capability on flavone. The method has low cost and reasonable process and can adapt to large-scale production.

Description

Technical field: [0001] The invention belongs to the technical field of plant extraction, and relates to a preparation method of total flavonoids of Cape japonica, in particular to a method for extracting and preparing total flavonoids of Cape ponica by adopting macroporous resin and nanofiltration technology. Background technique: [0002] Galium verum is the whole herb of Galium verum L., a plant of the genus Rubiaceae. Born in hillside shrubs and wilderness grasslands, distributed in the northeast, northwest to the Yangtze River Basin. The dried whole herb of fenugreek has a wide range of medicinal foundations in the folk. The medicinal use first began in "Rescue the Famine Materia Medica". Dermatitis, bruises, measles, gynecological diseases, etc. Foreign studies have shown that lotus seeds can be used to treat a variety of diseases, including lymphadenitis, tonsillitis, etc. It also has blood pressure-lowering, antipyretic effects, and can be used as an astringent; G...

Claims

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Application Information

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IPC IPC(8): A61K36/74A61P1/16A61P9/00A61P9/12A61P11/04A61P15/00A61P17/00A61P29/00
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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