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Novel method for simultaneously extracting caffeine, tea polyphenol and tea polysaccharide from tea using mechanochemical principle

A mechanochemical and caffeine technology, applied in the field of extracting caffeine, tea polyphenols and tea polysaccharides, to achieve the effects of improving purity, increasing yield and reducing oxidation

Inactive Publication Date: 2011-05-04
李奇峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Novel method for simultaneously extracting caffeine, tea polyphenol and tea polysaccharide from tea using mechanochemical principle
  • Novel method for simultaneously extracting caffeine, tea polyphenol and tea polysaccharide from tea using mechanochemical principle

Examples

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Embodiment 1

[0046]At room temperature, mix 10 g of crushed tea leaves with 1 g of potassium carbonate and 0.2 g of sodium borate, and grind the mixture in a ball mill for 10 min; take out the ground powder, add 20 mL of chloroform to the mixed tea powder for 3 times, stir for 10 min each time, and filter , combined the chloroform phases obtained by 3 times of treatment, and concentrated to obtain a mixture of chlorophyll and caffeine. The mixture was prepared by sublimation method to obtain caffeine; 20 mL of water and 2 mL of phosphoric acid were added to the tea solids obtained by elution with chloroform to adjust the pH value to ≤7, and divided into 5 Add 20 mL of ethyl acetate to elute the tea solids again, stir for 10 min, filter, collect 5 times of ethyl acetate eluate, concentrate to obtain tea polyphenols; add 100 mL of water to the tea solids treated by the above steps, and heat to Keep warm at 100°C for 20min, filter, add 300mL of ethanol, let stand for 24h, collect the precipita...

Embodiment 2

[0048] At room temperature, mix 10g of crushed tea leaves with 1.2g of potassium silicate and 0.2g of sodium borate, and grind the mixture in a ball mill for 10 minutes; take out the ground powder, and add 20mL of petroleum ether-ethyl acetate to the mixed tea powder in 5 times (Volume ratio 1: 1) mixed solutions, stirred for 10min respectively, filtered, combined the petroleum ether-ethyl acetate phase obtained from five treatments, and concentrated to obtain a mixture of chlorophyll and caffeine, which was prepared by sublimation method to obtain caffeine; - Add 10mL of water and 2mL of phosphoric acid to the tea solids obtained by ethyl acetate elution, adjust the pH to ≤7, add 20mL of absolute ethanol in 5 times to elute the tea solids again, stir for 10min, filter, collect 5 times of ethanol mobile phase concentration , to get tea polyphenols; add 100mL of water to the tea solids that have been processed through the above steps, heat to 100°C and keep warm for 20min, filte...

Embodiment 3

[0050] At room temperature, mix 10 g of crushed tea leaves with 1 g of potassium bicarbonate and 0.2 g of sodium borate, and grind the mixture in a ball mill for 10 min; take out the ground powder, and add 20 mL of petroleum ether-chloroform (volume ratio 1:1) mixed solution, stirred for 10 min respectively, filtered, combined the obtained petroleum ether-chloroform phases after five treatments, and concentrated to obtain a mixture of chlorophyll and caffeine, which was prepared by sublimation method to obtain caffeine; obtained by eluting with petroleum ether-chloroform Add 10 mL of water and 2 mL of phosphoric acid to the tea solids to adjust the pH value to ≤7, add 20 mL of absolute ethanol in 5 times to elute the tea solids again, stir for 10 min, filter, collect 5 times of ethanol mobile phase concentration, and obtain tea polyphenols; Add 100mL of water to the tea solids treated in the above steps, heat to 100°C and keep warm for 20min, filter, add 300mL of ethanol, let s...

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Abstract

A novel method for simultaneously extracting caffeine, tea polyphenol and tea polysaccharide from tea using mechanochemical principles comprises the following steps: firstly, mixing tea raw materials and solid reagents in a mass ratio of 1:0.01-20, fully pulverizing the mixture for 1 min-60 min, taking out the pulverized powder, soaking the pulverized powder under stirring 1-10 times for 1-60 min with an organic solvent in an amount of 1-20 times the mass of the tea raw materials, filtering, collecting organic phase eluted a plurality of times, concentrating to obtain a mixture of caffeine and chlorophyll which is used to prepare caffeine by a regular method; secondly, soaking the solid tea powder in which caffeine and the chlorophyll have been removed with water in an amount of 1-5 times the mass of tea, adding acids to adjust the pH value to be not more than 7, soaking the pulverized powder under stirring 1-10 times for 1-60 min with a 1 to 20-fold amount of an organic solvent, filtering, collecting the tea polyphenol-containing organic solvent phase during each filtration, drying to obtain tea polyphenol, and if necessary, further purifying the tea polyphenol by other methods; thirdly, adding the tea residue processed in above steps into water in an amount of 5-10 times the volume of the tea residue, extracting at a temperature of 100 DEG C, centrifuging the extract to obtain a supernatant, adding anhydrous ethanol in an amount of 3 times the volume of the supernatant, allowing tea polysaccharide to precipitate for 24 h, centrifuging, and drying the precipitate to obtain the tea polysaccharide.

Description

(1) Technical field [0001] The invention relates to a new method for simultaneously extracting caffeine, tea polyphenols and tea polysaccharides from tea leaves by using mechanochemical principles. (2) Technical background [0002] Tea polyphenols is the general term for polyhydric phenol derivatives in tea, accounting for 15% to 35% of the dry weight of tea; its chemical composition catechins accounts for about 70% of the total tea polyphenols, in addition to flavonoids and flavonoids Alcohols, anthocyanins and anthocyanins, phenolic acids and phenolic acids, etc. Tea polyphenols are an ideal natural antioxidant with a series of pharmacological functions such as anti-cancer, anti-aging, anti-radiation, scavenging free radicals in the human body, and lowering blood sugar and blood lipids. They are widely used in food processing, medical care, and daily chemical industries. Applications. [0003] In addition, tea leaves also contain other functional components. Caffeine has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D473/12C07G99/00C08B37/00
CPCY02P20/54
Inventor 李奇峰欧阳竞锋段为刚王力
Owner 李奇峰
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