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Method for preparing gelatin with protease degradation ossein

A protease and acid protease technology, applied in the field of gelatin preparation, can solve the problems of separation process burden, influence on gelatin quality, and high gelatin ash content, and achieve high energy utilization rate and equipment utilization rate, short gelatin extraction time, and uniform molecular structure. Effect

Inactive Publication Date: 2011-05-11
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the raw material for preparing gelatin is degreased and non-demineralized bone particles, some water-soluble miscellaneous proteins and a small amount of fat in the aggregate will still exist in the prepared gelatin, affecting the quality of gelatin
Although most of the inorganic salts can be removed by precipitation during the post-treatment process, the residual amount of inorganic substances is still as high as more than 5%. The separation process brought a great burden, and even caused gelatin to become a substandard product due to high ash content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Choose 100 grams of bone bone that has been degreased and demineralized in the traditional process, and add water to grind it into bone bone slurry. The mass and volume ratio of bone bone and water is 1g:5L; the particle size of bone bone in the bone bone slurry is 0.5~ 2mm;

[0028] 2. Add hydrochloric acid to adjust the pH of the reactant to 4; add 0.3 g of pepsin and react at 40°C for 2 hours;

[0029] 3. Add milk of lime to the reactant slurry to adjust the pH of the slurry to 9;

[0030] 4. Heat the reaction slurry to 60°C for 2 hours;

[0031] 5. Use cotton cake filtration to remove solid residues and obtain a clear gelatin solution;

[0032] 6. After the glue solution passes through the anion-cation exchange resin, it is concentrated and dried to obtain 72 grams of solid gelatin.

[0033] The performance index of gelatin is shown in Table 1.

[0034] Table 1. The physicochemical index of embodiment 1 gelatin

[0035] color

Embodiment 2

[0037] 1. Choose 100 grams of bone bone that has been degreased and demineralized in the traditional process, and add water to grind it into bone bone slurry. The mass-to-volume ratio of bone bone and water is 1g:2.5L; the particle size of bone bone in the bone bone slurry is 0.5 ~2mm;

[0038] 2. Add hydrochloric acid to adjust the pH of the reactant to 2.5; add 0.3 g of pepsin, and react at 50°C for 2 hours;

[0039] 3. Add milk of lime to the reactant slurry to adjust the pH of the slurry to 7;

[0040] 4. Heat the reaction slurry to 65°C for 1 hour;

[0041] 5. Use cotton cake filtration to remove solid residues and obtain a clear gelatin solution;

[0042] 6. After the glue solution passes through the anion-cation exchange resin, it is concentrated and dried to obtain 68 grams of solid gelatin.

[0043] The performance index of gelatin is shown in Table 2.

[0044] Table 2. The physicochemical index of embodiment 2 gelatin

[0045] color

Embodiment 3

[0047] 1. Choose 100 grams of bone bone that has been degreased and demineralized in the traditional process, and add water to grind it into bone bone slurry. The mass-to-volume ratio of bone bone to water is 1g:5L; the particle size of bone bone in the bone bone slurry is 0.5~ 5mm;

[0048] 2. Add phosphoric acid to adjust the pH of the reactant to 7; add 0.1 g of trypsin, and react at 37°C for 2 hours;

[0049] 3. Add milk of lime to the reactant slurry, and adjust the pH of the slurry to be 10;

[0050] 4. Heat the reaction slurry to 60°C for 2 hours.

[0051] 5. Use cotton cake filtration to remove solid residues and obtain a clear gelatin solution.

[0052] 6. After the glue solution passes through the anion-cation exchange resin, it is concentrated and dried to obtain 68 grams of solid gelatin.

[0053] The properties of gelatin are shown in Table 3

[0054] Table 3. The physicochemical index of embodiment 3 gelatin

[0055] color

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Abstract

The invention discloses a method for preparing gelatin with protease degradation ossein. The method is characterized in that derosination and demineralization ossein is mainly adopted as the raw material, and acidic or neutral protease degradation ossein is used for preparing gelatin. Compared with the traditional technology, the method has the advantage that long liming period and complicated neutralization washing process are omitted, so that the production period of gelatin is shortened, and a great amount of water resource and manpower cost are saved; and compared with the new technology of preparing gelatin with skeleton grains, and the content of foreign protein and inorganic acid in gelatin is greatly reduced, so that freezing strength and viscosity of the product are improved.

Description

technical field [0001] The invention belongs to the field of gelatin preparation, and in particular relates to a method for preparing gelatin by degrading osteoin with protease. Background technique [0002] At present, the production scale of my country's gelatin industry is expanding year by year, but there is still no new breakthrough in the production process of each gelatin factory. The production cycle is long, the energy consumption is high, and the difficulty of sewage treatment is a prominent problem, which is not in harmony with the sustainable development of society. . [0003] The process route for preparing gelatin in the traditional alkaline process is as follows: raw materials Smashed bones skim washed dry bone filter pickling washed liming washed neutralize washed extract filter concentrate dry Grinding, the advantage of the traditional alkaline process to prepare gelatin is that the reaction conditions of pickling, liming and e...

Claims

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Application Information

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IPC IPC(8): C09H3/00C12S3/16
Inventor 陈丽娟张兵史京京郭燕川
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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