Fluorescent detection method of benzo (a) pyrene in edible oil

A technology for fluorescence detection and edible oil, applied in fluorescence/phosphorescence, preparation of test samples, spectrum investigation, etc., can solve the problems of cumbersome processing, lower sensitivity, time-consuming, etc., and achieve short analysis time, good selectivity, and steps simple effect

Inactive Publication Date: 2011-05-25
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These analytical methods are not only cumbersome and time-consuming in the pretreatment process, but also expensive and consume solvents.
The chromatographic detection method inevitably has the dilution effect of the carrier, which relatively reduces the sensitivity, and is not suitable for the promotion of the method and the screening of large-scale samples of grass-roots testing institutions.

Method used

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  • Fluorescent detection method of benzo (a) pyrene in edible oil
  • Fluorescent detection method of benzo (a) pyrene in edible oil
  • Fluorescent detection method of benzo (a) pyrene in edible oil

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Sunflower oil: Weigh 0.5g of sunflower oil sample in a 25mL serum bottle, add 4mL of dimethyl sulfoxide, 59kHz, ultrasonic extraction for 6min under the conditions of 250W, take out and let stand for stratification, and separate the upper layer The oil layer was extracted once again under the same conditions, and the dimethyl sulfoxide extract was collected twice as the test solution. Pipette 2mL of the solution to the conventional quartz fluorescence sample cell of the fluorescence photometer, and perform constant energy simultaneous fluorescence spectrum mapping. The instrument parameters are set as follows: constant energy difference In terms of excitation wavelength, the scanning start wavelength is 260nm; the wavelength scanning range is 220nm. like figure 1 The zero-order-constant energy synchronous fluorescence spectrum of figure 1The benzo(a)pyrene spectral peak at 390nm can be used for the identification and rough determination of benzo(a)pyren...

Embodiment 2

[0028] Embodiment 2: Similar to Embodiment 1, the difference is that the second-order derivation function is added to obtain as figure 2 The second derivative-constant energy simultaneous fluorescence spectra are shown. Then add 4 μL of benzo(a)pyrene standard solution with a concentration of 1 mg / L to 2 mL of the sample solution, perform second derivative-constant energy simultaneous fluorescence spectrum mapping, and add the standard 4 times. Use the peak-zero method to read the signal intensity value of the sample at 401nm, and use it as the derivative fluorescence intensity for quantitative calculation. image 3 The standard addition curve, the linear fitting equation of the standard addition curve is Y=405.64+173.03X, the correlation coefficient is 0.9999, and the linearity is good. Thus, the content of benzo(a)pyrene in the sunflower oil sample extract was 2.34 μg / L, and the content of benzo(a)pyrene in sunflower oil was 37.4 μg / kg. Depend on figure 2 It can be seen...

Embodiment 3

[0029] Example 3: Blended oil: Weigh 0.5g blended oil sample into a 25mL serum bottle, add 4mL dimethyl sulfoxide solvent, extract under 59kHz, 250W ultrasonic conditions for 6min, take out and let stand for stratification, and separate the upper layer The oil layer was extracted once again under the same conditions, and the dimethyl sulfoxide extract was collected twice as the test solution. Pipette 2mL of the solution to the conventional quartz fluorescence sample cell of the fluorescence photometer, and perform constant energy simultaneous fluorescence spectrum mapping. The instrument parameters are set as follows: constant energy difference The scanning start excitation wavelength is 260nm; the wavelength scanning range is 220nm. get as Figure 4 The zero-order-constant-energy synchrofluorescence spectrum is shown. Adding the second-order derivation function, we get as Figure 5 The second derivative-constant energy simultaneous fluorescence spectra are shown. The ...

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Abstract

The invention discloses a fluorescent detection method of benzo (a) pyrene in edible oil, relates to a detection method of edible oil, and provides a fluorescent detection method of benzo (a) pyrene in edible oil, wherein the method is is simple, convenient and quick to operate, and the cost is low. The fluorescent detection method comprises the following steps: adding a dimethyl sulfoxide solvent in the edible oil; extracting and taking out the extracted product; s layering by standing or centrifuging; re-extracting the separated oil layer on the upper layer once, and collecting the dimethyl sulfoxide extraction liquid twice as liquid to be detected; plotting the liquid to be detected by a fluorescence spectrophotometer with derivative-constant energy isochronous scanning according to the following constant energy synchronizing fluorescence spectra conditions, and reading the fluorescence intensity of the benzo (a) pyrene by constant energy difference to identify the benzo (a) pyrene and measure roughly; and adding a second-order derivation function under the plotting conditions of the constant energy synchronizing fluorescence spectra to plot the derivative-constant energy synchronizing fluorescence spectra; reading the fluorescence intensity of the benzo (a) pyrene; and measuring the benzo (a) pyrene quantitatively by utilizing a continuous standard addition method.

Description

technical field [0001] The invention relates to a detection method of edible oil, in particular to a fluorescence detection method of benzo(a)pyrene in edible oil. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of organic pollutants that widely exist in the environment and in various foods. Due to their strong lipophilicity, edible oils are more susceptible to PAHs contamination. Grimmer and Hildebrandt revealed the PAHs content level in vegetable oil for the first time in 1968. PAHs can be produced by incomplete combustion or direct contact with pyrolysis gas during the drying process involving oilseeds. Oilseeds may also be contaminated by PAHs due to exposure to pollutants such as motor oil during mechanical harvesting, transportation, and processing. The content of PAHs has been found to be as high as 2000ppb or more in coconut oil. However, in some rural areas, due to the lack of dryers, during the harvest season, people can often s...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/34G01J3/30
Inventor 李耀群林丽容李秀英李呐
Owner XIAMEN UNIV
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