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Preparation method of extract of total triterpene acid of loquat leaf

An extraction method and a technology of loquat leaves, which are applied in the field of preparation of loquat leaf total triterpene acid extracts, can solve the problems of little increase in triterpene acid content, complicated process, and low triterpene acid content, and achieve the goal of increasing the total triterpene acid content. acid content, increase the content of triterpene acid, and improve the effect of product purity

Inactive Publication Date: 2012-01-04
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the preparation of loquat leaf total triterpene acid extract directly adopts solvent extraction, and the process is relatively simple, but the content of triterpene acid in the obtained extract is low; improving the process can appropriately increase the content of triterpene acid in the extract. content, but the process is relatively complicated, the cost is high, and the content of triterpene acid is increased less, which cannot meet the pharmaceutical demand

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  • Preparation method of extract of total triterpene acid of loquat leaf

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preparation example Construction

[0026] Preparation of the reference substance solution: Accurately weigh an appropriate amount of the triterpene reference substance and dissolve it in methanol to prepare 0.2 mg·ml of mallardic acid -1 , Potentinic acid 0.1mg·ml -1 , Maslinic acid 0.1mg·ml -1 , Crosolic acid 0.2mg·ml -1 , Oleanolic acid 0.05mg·ml -1 , Ursolic acid 0.2mg·ml -1 The mixed reference solution was used as the reference solution.

[0027] Preparation of sample solution: Accurately weigh 10.0 mg of loquat leaf total triterpene acid extract into a 10 ml volumetric flask, add methanol for ultrasonic dissolution, shake well, and filter through a 0.45 μm microporous membrane to obtain the solution.

[0028] Sample determination: Take 10 μl each of the sample solution and the reference solution, inject the sample, and calculate the sample content by the peak area according to the external standard method.

[0029] UV spectrophotometry:

[0030] Preparation of the reference solution: Accurately weigh...

Embodiment 1

[0037] Take 1.0kg of dried loquat leaves and grind them into coarse powder, cold-soak them twice with 80% ethanol 8L and 6L successively, each time for 15 days, combine the impregnating liquid, concentrate under reduced pressure to form a thick paste, add 80% ethanol 10 times the weight of the thick paste , stir and set aside. Filter and take the supernatant for later use. Take 5 kg of activated carbon particles and pack them into a column, load the above-mentioned standby supernatant, carry out gradient elution with 50 kg of 70% ethanol and 50 kg of 90% ethanol respectively, and collect the 90% ethanol eluate. Concentrate under reduced pressure to an alcohol concentration of 40%, stand for 8 hours for cold analysis, filter, precipitate and dry to obtain the total triterpene acid extract of loquat leaves with a yield of 1.2%. The extract was tested by HPLC, and the main triterpene acid components were as follows: 12.13% mallard acid, 8.88% policinic acid, 10.12% maslinic acid...

Embodiment 2

[0039]Take 1.0kg of dried loquat leaves and grind them into coarse powder, impregnate in 20kg of 90% ethanol for 48 hours, extract by percolation, and percolate until the color of the effluent liquid is light, and the triterpene reaction is not obvious, concentrate under reduced pressure to form a thick paste, add 30 times 90% ethanol by weight of the thick paste, stirred, placed in a centrifuge for sedimentation and centrifugation, and the supernatant was taken for subsequent use. Take 0.5 kg of activated carbon particles and pack them into a column, load the above-mentioned standby supernatant, elute with 4 kg of 90% ethanol, and collect the ethanol eluate. Concentrate under reduced pressure to an alcohol concentration of 20%, stand for 10 hours for cold analysis, filter, and dry the precipitate. The loquat leaf total triterpene acid extract was obtained, and the yield was 1.4%. The extract was detected by HPLC, and the main triterpene acid components were as follows: 15.61...

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Abstract

The invention discloses a preparation method of the extract of total triterpene acids of loquat leaves, and the method comprises the following steps: (1) drying and pulverizing the loquat leaves; (2) extracting the dried powder of the loquat leaves by using ethanol or methanol as a solvent, and concentrating the extracted liquid under reduced pressure to obtain an extract; (3) dissolving the extract obtained in step (2) by 80-90% ethanol or methanol, filtering or centrifuging the solution to remove insoluble substances so as to obtain an alcoholic solution; (4) filling the column with active carbon, loading the alcoholic solution obtained in step (3) into the column, performing gradient elution by ethanol or methanol, and collecting the eluate; (5) concentrating and drying the eluate collected in step (4) to obtain the extract of total triterpene acids of loquat leaves; or concentrating the eluate collected in step (4) to achieve an alcohol concentration of 20-50%, allowing the eluateto stand for 6-12 hours, filtering, drying the precipitates to obtain the extract of total triterpene acids of loquat leaves. The product prepared by the method has a high content of total triterpeneacids of loquat leaves, and the method has simple process and low cost.

Description

technical field [0001] The invention specifically relates to a preparation method of a loquat leaf total triterpene acid extract. Background technique [0002] Loquat leaf is the dried leaf of Eriobotrya japonica (Thunb.) Lindl., a plant of the genus Loquat in the family Rosaceae. It is a commonly used traditional Chinese medicine, and it is recorded in the Chinese Pharmacopoeia for clinical use in traditional Chinese medicine. Loquat leaves belong to the lung and stomach meridian, and have the functions of clearing the lung and relieving cough, lowering adverse flow and anti-vomiting. Recent studies have found that triterpenoids isolated from loquat leaves have significant hypoglycemic activity. [0003] At present, most of the preparation of loquat leaf total triterpene acid extract directly adopts solvent extraction, and the process is relatively simple, but the content of triterpene acid in the obtained extract is low; improving the process can appropriately increase th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61P1/08A61P11/14A61K127/00
Inventor 吕寒赵友谊陈剑罗美红李维林吴菊兰
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI