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Method for preparing duck virus hepatitis refined heterogenous yolk antibody

A duck viral hepatitis and egg yolk antibody technology is applied in the direction of antibodies, antiviral agents, digestive system, etc. It can solve the problems of clinical diagnosis and prevention, mixed infection, etc., and achieve stable properties, high potency, and easy operation Effect

Active Publication Date: 2011-06-01
南通市鑫海水产养殖专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Duck viral hepatitis is a highly lethal and rapidly spreading acute infectious disease of ducklings caused by duck hepatitis virus (DHV). It is one of the most serious diseases in the duck industry and is often mixed with viruses and bacteria. It has brought great difficulties to clinical diagnosis and prevention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for preparing duck viral hepatitis refined heterologous egg yolk antibody, the specific steps are:

[0040] (1) Separation of high-free eggs and yolks: collect high-free eggs and use 70% (V / V) ethanol to disinfect the surface of the eggshells, and send them to the egg white and yolk separator after drying to separate the egg yolks;

[0041] (2) Extract egg yolk antibody:

[0042] A: Mix the egg yolk collected in step (1) with sterile water 1:1 (V / V) and stir evenly, seal and inactivate at 65°C for 10 minutes, then quickly cool to 10°C, and then mix with sterile water at a ratio of 1:1 Mix in the ratio of 4 (V / V), adjust the pH to 5.2 with 1M HCl, stir well, let stand at 4°C for 6 hours, centrifuge at 5000rpm for 20 minutes, and take the supernatant;

[0043] B: Mix the supernatant obtained in step A with the saturated ammonium sulfate solution in a ratio of 1:1 (V / V), the mixing method is to gradually add the saturated ammonium sulfate solution to the supernat...

Embodiment 2

[0048] A method for preparing duck viral hepatitis refined heterologous egg yolk antibody, the specific steps are:

[0049] (1) Use Peptide Easy B-001 poultry special preparation to feed healthy laying hens continuously for 3 weeks, add once a day, and the daily addition amount is 0.2% of the basic diet.

[0050] (2) Prepare the oil emulsion antigen for immunization:

[0051] A: Dilute the frozen duck hepatitis virus AV2111 seed (purchased from China Veterinary Drug Control Institute) 200 times and inoculate 10-11 day-old SPF chicken embryos (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.), the inoculation volume 100 microliters per bird, incubate in a 37°C incubator, collect dead chicken embryos after 48-96 hours, and collect chicken embryo allantoic fluid with obvious embryo body lesions;

[0052] B: The allantoic fluid collected in step A was added to formalin with a final concentration of 0.1% to seal and inactivate at 37°C for 48 hours, and...

Embodiment 3

[0063] In step (5) A, mix the egg yolk collected in step (4) with sterile water 1:1 (V / V) and stir evenly, seal and inactivate at 65°C for 10 minutes and then cool rapidly to 8°C; the rest of the steps are implemented in the same way Example 2.

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Abstract

The invention provides a method for preparing a duck virus hepatitis refined heterogenous yolk antibody, comprising the following specific steps: (1) separating the yolk of a high-immunity egg; (2) extracting a yolk antibody; and (3) preparing a preparation and packaging. The method for preparing the duck virus hepatitis refined heterogenous yolk antibody is easy to operate and is applicable to mass production, and the prepared duck virus hepatitis yolk antibody has the advantages of stable property, high purity, high valence, stronger specificity and no drug residue, is mild and environmentally friendly and has application value in preventing and treating duck virus hepatitis, thus a new way is provided for preparing the duck virus hepatitis refined heterogenous yolk antibody.

Description

technical field [0001] The invention relates to the technical field of biological products for poultry, in particular to a preparation method of refined heterologous yolk antibody against duck viral hepatitis. Background technique [0002] Duck viral hepatitis is a highly lethal and rapidly spreading acute infectious disease of ducklings caused by duck hepatitis virus (DHV). It is one of the most serious diseases in the duck industry and is often mixed with viruses and bacteria. It has brought great difficulties to clinical diagnosis and prevention. DHV-I mainly infects ducklings under 1 month old, and ducklings within two weeks are especially susceptible. The morbidity rate of ducklings is 100%, and the mortality rate is different because of the age of the ducklings. The mortality rate of ducklings less than one week old can reach 95%, and the mortality rate of ducklings at the age of 1-3 is 50% or lower. Adult ducks can be infected without getting sick, and their egg pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61P1/16A61P31/20
Inventor 陈业金范文辉张树伟李小双秦文安
Owner 南通市鑫海水产养殖专业合作社
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