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Double enzyme method for preparing zinc gluconate

A technology of zinc gluconate and double-enzyme method, which is applied in the field of preparing zinc gluconate by double-enzyme method, which can solve the problems of complex process route, high cost of separation and handover, and low purity of zinc gluconate products, and achieve simple process and high product purity. high effect

Inactive Publication Date: 2012-11-14
SHANDONG XIWANG SUGAR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The production of zinc gluconate by Aspergillus niger fermentation requires sterilization of sugary raw materials, which will make it difficult to decolorize the fermentation broth; the fermentation ingredients need to add a variety of nutrient salts and introduce impurities, which will increase the cost of purification
And gained zinc gluconate product purity is lower
And it needs to be fermented to produce calcium gluconate first, and then use calcium gluconate as an intermediate to produce zinc gluconate. The additives used are harmful to the human body

Method used

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  • Double enzyme method for preparing zinc gluconate
  • Double enzyme method for preparing zinc gluconate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Prepare 6000 ml of 20% (mass to volume ratio W / V) glucose solution, add 280 g of zinc oxide, adjust the pH to 7.0, add successively 0.8% catalase and 1.0% glucose oxidation Enzyme, set the enzyme reaction temperature at 35°C, pass in sterile air, control the ventilation rate at 1:1v / v / min, keep the pressure of the reaction tank at 0.1MPa, and the reaction cycle is 40 hours. When the concentration of reducing sugar in the reaction solution When it drops to 0, the reaction is terminated.

[0018] The temperature of the reaction solution was raised to 95°C to kill the enzyme, and 0.1% (W:V) of activated carbon was added to decolorize at 60°C for 1 hour, and the filtrate was filtered to obtain the filtrate, which was evaporated at 60°C under a vacuum of 0.095MPa. The mass percentage of dry matter was 22% stopped. Be down to 24 ℃ with the speed of 1.5 ℃ / hour by 60 ℃, the crystallization cycle of cooling is 24 hours, separates zinc gluconate crystal with centrifuge. The obt...

Embodiment 2

[0020] Prepare 6000 ml of 15% (mass volume ratio W / V) glucose solution, add 220 g of zinc oxide, adjust the pH to 7.0, add successively 0.8% catalase and 1.0% glucose oxidase , set the enzyme reaction temperature to 35°C. Introduce sterile air, control the ventilation rate to 1:1v / v / min, keep the pressure of the reaction tank at 0.1MPa, and the reaction period is 35 hours. When the reducing sugar concentration in the reaction solution drops to 0, the reaction is terminated.

[0021] The temperature of the reaction solution was raised to 95°C to inactivate the enzyme, 0.1% (W:V) was added to activated carbon for decolorization at 60°C for 1 hour, and the filtrate was obtained by filtration, and evaporated at 60°C under a vacuum of 0.095MPa. The mass percentage of dry matter was 20% off. Be down to 24 ℃ with the speed of 1.5 ℃ / hour by 60 ℃, the crystallization cycle of cooling is 24 hours, separates zinc gluconate crystal with centrifuge. The obtained crystals were dried at 80...

Embodiment 3

[0023] Prepare 6000 ml of 15% (mass volume ratio W / V) glucose solution, add 220 g of zinc oxide, adjust the pH to 7.0, add successively 0.8% catalase and 1.0% glucose oxidase , set the enzyme reaction temperature to 35°C. Introduce sterile air, control the ventilation rate to 1:1v / v / min, keep the pressure of the reaction tank at 0.2MPa, and the reaction period is 25 hours. When the reducing sugar concentration in the reaction solution drops to 0, the reaction is terminated.

[0024] The temperature of the reaction solution was raised to 95°C to inactivate the enzyme, and 0.1% (W:V) of activated carbon was added to decolorize at 60°C for 1 hour, and the filtrate was obtained by filtration, and evaporated at 60°C under a vacuum of 0.095MPa. The mass percentage of dry matter was 20% off. Be down to 24 ℃ with the speed of 1.5 ℃ / hour by 60 ℃, the crystallization cycle of cooling is 24 hours, separates zinc gluconate crystal with centrifuge. The obtained crystals were dried at 80°...

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Abstract

The invention discloses a production process of zinc gluconate, which comprises the following production steps: (1) preparing glucose until the concentration of the glucose is 10-25% (W / V), and then, putting the glucose into a reaction tank; (2) firstly adding zinc oxide the additive amount of which accounts for 45-50% of the glucose dry basis into the reaction tank, then adding catalase the additive amount of which accounts for 0.3-1.2% of the glucose dry basis, and then adding glucose oxidase the additive amount of which accounts for 0.5-1.5% of the glucose dry basis; (3) introducing sterile air into the reaction tank, wherein the ventilation (VVm) is 0.6-1.2, the stirring rate is 450-500 revolutions / minute, and the enzyme reaction temperature is 32-39 DEG C; (4) carrying out reaction for 25-40 hours, and finishing the reaction when the concentration of reducing sugar in reaction liquid is reduced to 0; and (5) inactivating the enzymes in the reaction liquid, discoloring, concentrating until the concentration of dry matters is 20-40%, cooling and crystallizing, and then, separating and drying to obtain a finished zinc gluconate product. The invention has the advantage of simple process. The zinc gluconate product produced by the method has high purity and meets the current national requirements for energy saving and environment protection of enterprises.

Description

technical field [0001] The invention belongs to the technical field of enzymatically producing zinc gluconate, in particular to a method for preparing zinc gluconate by a dual-enzyme method. Background technique [0002] Zinc is one of the essential trace elements for the human body, which can enhance the body's immunity and promote the growth and development of children. Zinc gluconate is the most advantageous product in zinc supplements. Zinc gluconate is also widely used in cosmetics. Zinc gluconate is white crystal or granular powder; easily soluble in water, insoluble in ethanol, chloroform and ether. [0003] There are generally two traditional production methods for zinc gluconate, one is to use Aspergillus niger to produce gluconic acid and then neutralize to produce zinc gluconate through aerobic fermentation process; the other is to use glucose as raw material to generate glucose through catalyst oxidation The acid is then neutralized to produce zinc gluconate. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/58
Inventor 王勇阿纳斯谢登玉李曰强李秋红尹玉健
Owner SHANDONG XIWANG SUGAR