Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compositions and methods for identifying response targets and treating flavivirus infection responses

A flavivirus and composition technology, applied in antiviral agents, compound screening, chemical instruments and methods, etc., can solve the problem of difficulty in knowing whether the extract contains polysaccharides, etc.

Inactive Publication Date: 2011-06-01
ACAD SINIC
View PDF36 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is still difficult to know whether the extract contains the active ingredients of polysaccharides based on mass spectrometry

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compositions and methods for identifying response targets and treating flavivirus infection responses
  • Compositions and methods for identifying response targets and treating flavivirus infection responses
  • Compositions and methods for identifying response targets and treating flavivirus infection responses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] Example 1: Innate immune receptor: preparation of Fc fusion protein

[0135] cell culture

[0136] Place 293F cells (Invitrogen, R790-07) in a 125 mL flask placed on a rotary shaker (125 rpm) in serum-free 293FREESTYLE 203 expression medium (Invitrogen, 12338-018), at 37°C in CO 2 Cultivated in an incubator.

[0137] Construction of receptor Fc fusion gene

[0138] The extracellular regions of lectin receptors, TREMs and TLTs, were cloned by reverse transcriptase polymerase chain reaction (RT-PCR), followed by subcloning into yT&A vector, and then into pcDNA3.1(+)hIgG1. Fc expression vector. The resulting receptor.Fc construct encodes a recombinant protein fused to a mutated human IgGl Fc portion that does not bind to the human Fc receptor. The mutations in the IgGl Fc portion are L234A, L235E, G237A and P331S. The sequences of the primers used to amplify the extracellular region by RT-PCR are as follows (or primers can be selected from the sequences listed in Ta...

Embodiment 2

[0267] Embodiment 2: Preparation of polysaccharide extract

[0268] Ganoderma lucidum crude extract

[0269] Crude Ganoderma lucidum extract (prepared by alkaline extraction, neutralization and ethanol precipitation) was obtained from Pharmanex (CA, USA). Unless otherwise indicated, the molecular weight cut-off (MWCO) is between 6000-8000 Daltons Tubular dialysis membrane, Thermo bio-basic SEC-1000 column, Tosoh TSK G5000PWx1SEC column, and all chemicals and reagents were obtained from Sigma or Aldrich.

[0270] Purification of Ganoderma lucidum extract

[0271] Crude Ganoderma lucidum powder (6g) (obtained from Pharmanex company) was dissolved in 120ml of ddH 2 O, stirred in boiling water (100° C.) for 2 hours and centrifuged (1000 rpm) for 1 hour to remove insoluble matter. The resulting solution was concentrated between about 40°C and about 50°C to obtain a small volume, then lyophilized to yield 5 g (83%) of a dark brown powder (Ganoderma lucidum polysaccharide; GLP...

Embodiment 3

[0284] Example 3: Purified Receptor: Western Blot Analysis of Fc Fusion Proteins

[0285] The purified receptor-Fc fusion protein of Example 1 was subjected to electrophoresis, transferred to a nitrocellulose membrane (Hybond-C extra, Amersham Pharmacia Biotech), and mixed with (1:3000) in TBST buffer (5% delipidated Dry milk, peroxidase-conjugated goat anti-human IgG Ab (Jackson, PA, USA) reaction in Tris-buffered saline containing 0.02% Tween 20). After rinsing with TBST, blots were incubated with enhanced chemiluminescence reagent (Amersham Pharmacia Biotech) for visualization.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

Cellular receptors are identified that induce plasma leakage and other negative effects when infected with flaviviruses, such as dengue virus or Japanese encephamyelitis virus. Using fusion proteins disclosed herein, the receptors to which a pathogen, such as flavivirus, binds via glycan binding are determined. Once the receptors are determined, the effect of binding to a particular receptor may be determined, wherein targeting of the receptors causing a particular symptom may be targeted by agents that interrupt binding of the pathogen to the receptor. Accordingly, in the case of dengue virus and Japanese encephamyelitis virus, TNF-a is released when the pathogen binds to the DLVR1 / CLEC5A receptor. Interrupting the DLVR1 / CLEC5A receptor with monoclonal antibodies reduced TNF-alpha secretion without affecting secretion of cytokines responsible for viral clearance thereby increasing survival rates in infected mice from nil to around 50%.

Description

[0001] Related applications [0002] This application claims Paris Convention priority of U.S. Patent Application No. 12 / 079,576 (filed March 27, 2008) and U.S. Patent Application No. 11 / 469,270 (filed Aug. 31, 2006), which claim U.S. The Paris Convention priority of provisional application No. 60 / 713,463 (filed on August 31, 2005), and the disclosure content of the above-mentioned applications are incorporated in this specification in full by reference. [0003] This invention was supported by programs 94F008-5, NSC 95-2320-B-010010, and NSC 95-3112-B-010-017 of the National Science Council of Taiwan. This invention was also supported by projects 94M002-1 of Academia Sinica and 95A-CT8G02 of National Yang Ming University. Background technique [0004] Reference in this specification to any prior art is not or should not be considered as an acknowledgment or in any way to suggest that such prior art forms part of the general knowledge in any country. All documents cited here...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N2500/02A61K2039/505C07K2319/30C07K2316/96G01N2333/18C07K2317/56G01N2333/185C07K16/2851C07K2317/76A61P31/12A61P31/14Y02A50/30A61K39/39533A61K39/3955
Inventor 谢世良翁启惠徐翠玲陈斯婷
Owner ACAD SINIC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com