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Method for enhancing control effect of biological control agent for plant parasitic nematodes

A technology for biocontrol agents and nematodes, applied in plant growth regulators, botany equipment and methods, nematocides, etc., can solve the problems that biocontrol bacteria cannot be promoted on a large scale, affect the application of biocontrol agents, and have low germination rates , to achieve stable control effects, easy industrial production, and good comprehensive properties

Inactive Publication Date: 2011-06-15
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The root cause is the influence of the ecological environment, among which the inhibitory effect of the soil on the production of bio-control bacteria is one of the most important influencing factors, which is also the bottleneck that restricts the large-scale promotion of many soil-borne diseases and insect pest bio-control bacteria
Due to the existence of soil antibacterial effect, the germination rate of fungal spores in the soil is very low, generally between 0-40%, and rarely more than 50%. Different types of soil have different antifungal effects, which eventually lead to biocontrol Bacteria establish populations of different densities in the soil, so the same preparation reflects different control effects in different regions and different types of soil, which directly affects the application of biocontrol preparations
Field germination of fungicides has become a bottleneck restricting the efficacy and use of biocontrol fungicides

Method used

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  • Method for enhancing control effect of biological control agent for plant parasitic nematodes

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] 1. Cultivate the biocontrol bacteria by a conventional liquid culture method to obtain a liquid culture including mycelia, molecular spores, and chlamydospores, and the amount of spores is determined according to the specific requirements of each biocontrol agent;

[0022] 2. Add sodium alginate to the liquid culture at 1‰ of the liquid culture and mix well;

[0023] 3. Continue to add diatomite with an adsorption capacity of 1:1.2 (based on water) and a particle size of 50 μm to the above mixture, and mix well for no less than one hour;

[0024] 4. Continue to add polyvinyl alcohol to the above mixture according to 1‰ of the mixed powder, and mix well for no less than half an hour;

[0025] 5. According to the specific requirements of each biocontrol agent, dry, pack and set aside.

example 2

[0027] 1. Cultivate the biocontrol bacteria by a conventional liquid culture method to obtain a liquid culture including mycelia, molecular spores, and chlamydospores, and the amount of spores is determined according to the specific requirements of each biocontrol agent;

[0028] 2. Add thermocoagulable polysaccharide in the liquid culture according to 5‰ of the liquid culture, and mix well;

[0029] 3. Continue to add porous peat with an adsorption capacity of 1:1.4 (based on water) and a particle size of 200 μm to the above mixture, and mix well for no less than one hour;

[0030] 4. Continue to add polyvinyl alcohol to the above mixture according to 4‰ of the mixed powder, and mix well for no less than half an hour;

[0031] 5. According to the specific requirements of each biocontrol agent, dry, pack and set aside.

example 3

[0033] 1. Cultivate the biocontrol bacteria by a conventional liquid culture method to obtain a liquid culture including mycelia, molecular spores, and chlamydospores, and the amount of spores is determined according to the specific requirements of each biocontrol agent;

[0034] 2. Add carrageenan to the liquid culture according to 1% of the liquid culture, and mix well;

[0035] 3. Continue to add porous vermiculite with an adsorption capacity of 1:6 (based on water) and a particle size of 300 μm to the above mixture, and mix well for no less than one hour;

[0036] 4. Continue to add polyvinyl alcohol to the above mixture according to 7‰ of the mixed powder, and mix well for no less than half an hour;

[0037] 5. According to the specific requirements of each biocontrol agent, dry, pack and set aside.

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Abstract

The invention relates to a preparation method for enhancing the control effect of a biological control agent for plant parasitic nematodes, belonging to the technical field of biological pesticide. The method of the invention enhances the field survival rate, the anti-germination inhibiting effect and the anti-environment capability of the biological control agent by adding the biological control agent with sodium alginate, curdlan, carrageenin, starch or cyclodextrin cell protective agent, diatomite, crushed crop straw, porous grass carbon, porous vermiculite, porous activated carbon or perlite powder cell attachment agent, and polyvinyl alcohol, carrageenin or gelatin cell immobilizing agent, thereby improving the control effect of the biological control agent. According to the method, other properties, using way and preservation way of the biological control agent are not changed, the biological control agent can be used with other nematocide pesticides and plant growth regulating agents together, however, the mixed agent must be guaranteed to lead no side effect to the biological control agent. The method of the invention has the advantages of obviously stable control effect, good comprehensive property and great easiness for industrialized production.

Description

Technical field: [0001] The invention relates to a method for improving the control effect of plant parasitic nematode biocontrol agents, belonging to the technical field of biological pesticides. Background technique: [0002] Plant parasitic nematodes are a common plant disease worldwide. There are more than 70 known types of root-knot nematodes alone, which damage more than 3,000 plants and cause huge losses every year. Plant parasitic nematodes in my country mainly harm economic crops such as tobacco, flowers, vegetables, cotton, soybeans, peanuts, Panax notoginseng, American ginseng, etc., and the losses caused are astonishing. According to incomplete statistics from the National Tobacco Pest Control Information Center, in 2000, the area of ​​tobacco root-knot nematode disease in the country alone had reached 500,000 hectares, and the direct economic loss was more than 500 million yuan. The affected area in Yunnan Province alone reached 27,000 hectares, and the direct ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/22A01P5/00
Inventor 乔敏余泽芬张克勤周薇
Owner YUNNAN UNIV