Surface enhanced Raman spectrum detection method for antioxidant BHT (Butylated Hydroxytoluene)

A surface-enhanced Raman and antioxidant technology, used in Raman scattering, measurement devices, preparation of test samples, etc., can solve the problems of low sensitivity, high operation requirements, long analysis time, etc., to improve sensitivity and reduce detection. The effect of limited and large-scale detection

Inactive Publication Date: 2011-06-15
JIANGNAN UNIV
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Problems solved by technology

[0003] At present, the detection methods of such antioxidants mainly include gas chromatography, capillary electrophoresis, high performance liquid chromatography, ultra-high performance liquid chromatography-mass spectrometry, etc., but the operation requirements are high, the analysis time is long, and the sensitivity is low.

Method used

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  • Surface enhanced Raman spectrum detection method for antioxidant BHT (Butylated Hydroxytoluene)
  • Surface enhanced Raman spectrum detection method for antioxidant BHT (Butylated Hydroxytoluene)
  • Surface enhanced Raman spectrum detection method for antioxidant BHT (Butylated Hydroxytoluene)

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preparation example Construction

[0031] (1) Preparation of nano-gold sol: Nano-gold sol can be prepared by common methods, for example, weigh chloroauric acid or its sodium salt, configure it into a solution with ultrapure water, stir and boil, quickly add sodium citrate solution, continue Stir and cool to normal temperature to obtain nano-gold sol, which can be placed for future use.

[0032] (2) Drawing of standard curve

[0033] Dissolve the BHT standard product in methanol to make a series of BHT standard solutions, and mix them with the nano-gold sol (the concentration of the nano-gold particles contained in the standard solutions of each concentration can be equal or a series of concentrations can also be used), and the surface-enhanced pull Mann spectrum was used for detection, and a standard curve was drawn according to the detection results. Among them, the setting conditions of the Raman spectrometer can be determined according to the characteristics of BHT on the surface-enhanced Raman spectrum (B...

Embodiment 1

[0044] Cut the PVC material into fragments of about 0.5cm×0.5cm, accurately weigh 0.5g from the fragments, add 10mL of acetonitrile or ethyl acetate and ultrasonically extract to dissolve all the samples, then add methanol to make the solution volume to 25mL, and wait for the solution to After the precipitation stops, filter the filtrate to dryness at 40°C, dilute to 2 mL with methanol, filter through a 0.45 μm filter membrane, then add 2 ml of nano-gold sol, and perform Raman spectrum detection under the aforementioned conditions. Three parallel samples were made for the PVC material, namely parallel sample 1, parallel sample 2, and parallel sample 3. The analysis results are shown in Table 1. The parallel sample 1 was repeatedly measured 6 times, and the analysis results are shown in Table 2. It can be seen from the analysis that the relative standard deviation (RSD) of the parallel samples is 8.13, and the equivalent standard deviation of the repeated measurement experiment...

Embodiment 2

[0050] Accurately weigh 2mL of edible oil, add 20mL of chloroform and mix well, then let it stand still to separate the chloroform solution; repeat the above operation twice, combine the chloroform solution obtained each time, and dehydrate it with anhydrous sodium sulfate Finally, it was evaporated to dryness at 70°C, and the volume was adjusted to 2mL with methanol, filtered through a 0.45μm filter membrane, and then 2ml of nano-gold sol was added, and Raman spectrum detection was carried out under the aforementioned conditions. Three parallel samples were made for the edible oil sample, namely parallel sample 1, parallel sample 2, and parallel sample 3. The analysis results are shown in Table 3. Parallel sample 1 was repeated 6 times, and the analysis results are shown in Table 4. Through analysis, the relative standard deviation (RSD) of the parallel samples is 0.686, and the relative standard deviation of the repeated measurement experiment is 0.346%, which shows good rep...

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Abstract

The invention relates to a surface enhanced Raman spectrum detection method for antioxidant BHT (Butylated Hydroxytoluene), which comprises the steps of: adopting gold nano particles as an enhancing reagent, and mixing the gold nano particles with an antioxidant BHT test sample solution for carrying out surface enhanced Raman spectrum detection so as to measure the content of antioxidant BHT in a test sample. The surface enhanced Raman spectrum detection method is simple and convenient to operate, has high analyzing speed and high sensitivity, is applicable to large-scale BHT detection, and is expected to be widely applied in the fields of food, medicine and the like.

Description

technical field [0001] The invention relates to a method for detecting antioxidant BHT in food and food packaging materials in the field of food safety detection, in particular to a method for detecting surface antioxidant BHT in food or food packaging materials by surface-enhanced Raman spectroscopy. Background technique [0002] Butylated hydroxytoluene (BHT) is a good antioxidant that can be used in food and food packaging materials. BHT can inhibit or delay the oxidative degradation of plastic or rubber to prolong the service life, prevent the acid value or viscosity of lubricating oil and fuel oil from increasing, and is also a stabilizer for synthetic rubber and a commonly used phenolic anti-aging agent. As a food additive, BHT can delay the rancidity of food, but excessive use of antioxidants has certain toxic and carcinogenic effects on the human body. GB9685-2008 stipulates that the maximum usage of BHT in food packaging materials is 0.13% and the specific migratio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N21/01G01N1/28G01N1/34
Inventor 姚卫蓉孙莹莹汪仕韬汪何雅钱和
Owner JIANGNAN UNIV
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