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Method for clarifying green tea extract liquid

A green tea extract, green tea technology, applied in tea extraction, tea, food science and other directions, can solve problems such as product yield, quality impact, etc., achieve the effects of good clarity, easy industrialization and application, and less equipment requirements

Inactive Publication Date: 2011-08-10
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of clarifying agent helps to remove the impurities of the raw material extract, it may also remove the effective active ingredients, which may affect the yield and quality of the product

Method used

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  • Method for clarifying green tea extract liquid
  • Method for clarifying green tea extract liquid
  • Method for clarifying green tea extract liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 The preparation method of green tea extract of the present invention

[0025] Take green tea powder, add 20 times the amount of 50% ethanol, extract twice at 80°C for 0.5 hours each time, filter, combine the filtrates, recover the ethanol under reduced pressure until there is no alcohol smell, and use the alcohol extract for later use; weigh ZTC1+1II Type A clarifier, add deionized water, swell, stir, and prepare 1% viscose solution; weigh ZTC1+1II type clarifier B component, add 1% acetic acid solution, swell, stir, and prepare 1% Viscose liquid; dilute the spare alcohol extract to the crude drug concentration of 1:20 crude drug volume ratio, at 80°C, stir and disperse the B component (ZTC1+1II type clarifying agent purchased from Tianjin Zhengtiancheng Clarification Technology Co., Ltd.), then stirred once every 30min, and after 2h, the temperature of the medicinal solution was lowered to 60°C, and the A component (ZTC1+1II type clarifier) ​​of 4% volume ...

Embodiment 2

[0026] Embodiment 2 The extraction method screening of green tea extract of the present invention

[0027] 1 Investigation of extraction solvent

[0028] Tea polyphenols are easily soluble in water, ethanol, methanol, acetone, ethyl acetate and other solvents. In this experiment, the content of tea polyphenols (GB / T8313-2002) was used as the evaluation index to investigate the effects of water and different concentrations of ethanol on tea polyphenols. Extraction efficiency, screening the best extraction solvent. See Table 1:

[0029] Table 1 Extraction solution, selection of extraction parameters

[0030]

[0031] Table 2 Results of extraction solution

[0032]

[0033] From the above results, combined with actual production, it is determined that 50% ethanol is the extraction solvent. Judging from the color of the extract, when hot water extraction is used, tea polyphenols, especially catechins, are easily oxidized to form theaflavins and thearubigins, which may be...

Embodiment 3

[0065] Embodiment 3 The clarification method screening of green tea extract of the present invention

[0066] 1. Instruments and reagents:

[0067] Shimadzu LC 10-ATVP high performance liquid chromatograph; BP211D electronic balance (1 / 10,000, 1 / 100,000, Sartouris Co., Ltd., Germany); TL-500 desktop centrifuge (Shanghai Institute of Centrifugal Machinery); electronic Constant temperature water bath (Beijing Guohua Medical Instrument Factory); UV1100 ultraviolet-visible spectrophotometer (Shanghai Tianmei Scientific Instrument Co., Ltd.); ZTC1+1 II clarifier (Tianjin Zhengtiancheng Clarification Technology Co., Ltd.); methanol (HPLC grade) Acetonitrile (HPLC grade) was purchased from Tedia Corporation of the United States, and other reagents were of analytical grade.

[0068] EGC, EC, EGCG, ECG reference substance (purchased from Chengdu Master Company) (EGCG: epigallocatechin gallate; ECG: epicatechin gallate; EGC: epigallocatechin; EC: epicatechin)

[0069] 2. Methods and ...

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Abstract

The invention provides a method for clarifying a green tea extract liquid, and a ZTC1+1 II type clarifying agent is adopted for clarifying the green tea extract liquid. The invention further provides the green tea extract liquid prepared by the method. By adopting the clarifying method, the using amount of the ZTC1+1 II type natural clarifying agent for clarifying the green tea extract liquid is small, the cost is low, active ingredients can be effectively retained, the prepared green tea extract liquid has excellent taste and good clarity, and the clarifying method has low equipment requirement and is easy to perform industrial popularization and application.

Description

technical field [0001] The invention relates to a method for clarifying green tea extract. Background technique [0002] Deep processing of tea is an effective measure to infiltrate the development and utilization of tea resources into functional food, health care products, pharmaceuticals, and daily chemical products. Clarification is one of the important processes in the deep processing of tea. Its purpose is to remove a large amount of macromolecular components such as tannin, protein, chlorophyll, pectin and suspended solids in the green tea extract. The degree of clarification directly affects the color of the final product. , cold solubility and yield. [0003] At present, the clarification processes of green tea extract mainly include: centrifugal clarification, microfiltration, ultrafiltration, nanofiltration, ceramic membrane, polyamide, PVPP, β-cyclodextrin, pectinase, immobilized tannase, etc. Filtration, suction filtration, centrifugation, microfiltration, ultr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/20A23F3/16
Inventor 刘友平黄丽凤陈鸿平陈林赵祎姗
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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