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Protein related to insect resistance and encoding gene and application thereof

An encoding gene and insect resistance technology, applied in the field of insect resistance-related proteins and their encoded genes and applications, can solve problems such as the rampant pest resistance, environmental pollution, and pesticide residues.

Active Publication Date: 2013-04-03
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While the use of chemical pesticides has achieved certain economic benefits, it has also brought serious problems such as pesticide residues, environmental pollution, ecological damage, pest resistance and rampant

Method used

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  • Protein related to insect resistance and encoding gene and application thereof
  • Protein related to insect resistance and encoding gene and application thereof
  • Protein related to insect resistance and encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Cloning of target gene and acquisition of overexpressed SlNAC072 transgenic tomato

[0035] 1. Cloning of the target gene

[0036] The total RNA of tomato (Solanum lycopersicon) variety M82 was extracted, and its reverse transcription product was used as a template for PCR amplification under the guidance of primers 1 and 2.

[0037] Primer 1: 5′-TCT GGTACC ATGGGTGTTCAAAGAAAAAAGATCCAC-3' (upstream primer, the base underlined is the Kpn I recognition site)

[0038] Primer 2: 5′-CAG CTGCAG CTACTGCTTGAACCCGAGATTTAAC-3' (downstream primer, the base underlined is the Pst I recognition site)

[0039] After the reaction, the PCR product was purified and connected to the pMD18-T vector, transformed into Escherichia coli DH5α, and positive clones were selected, and the positive clones were cultured in liquid, and the positive plasmid was extracted for sequencing. The result was inserted into pMD18-T with The genes shown in nucleotides 28-1077 in SEQ ID NO: 1 of ...

Embodiment 2

[0079] Example 2, Verification of the transcriptional activation activity of SlNAC072

[0080] 1. Cloning of the target gene

[0081] The vector pGBKT7 was purchased from Clontech, USA, the product catalog number is 630442. pGADT7 was purchased from Clontech, USA, the product catalog number is 630443.

[0082] According to the cDNA sequence of SlNAC072 and the suitable restriction site of the vector pGBKT7, primers were designed to amplify the full-length coding sequence of SlNAC072, the N-terminal coding sequence and the C-terminal coding sequence. The primer sequences are as follows:

[0083] Primer 5: 5'- CATATG GGTGTTCAAGAAAAAGA-3' (full-length coding sequence upstream primer, the underlined part of the base is the Nde I recognition site)

[0084] Primer 6: 5'- CTGCAG CTACTGCTTGAACCCGAGAT-3' (full-length coding sequence downstream primer, the underlined part of the base is the Pst I recognition site)

[0085] Primer 7: 5'- CATATG GGTGTTCAAGAAAAAGA-3' (N-terminal c...

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Abstract

The invention discloses a protein related to insect resistance and an encoding gene and application thereof. The protein is one of the following proteins in (1) and (2): (1) a protein shown in SEQ ID NO: 2; and (2) a protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on an amino acid residue sequence shown in SEQ ID NO:2, is related to insect resistance of plants and is derivative from the protein in (1). A method for cultivating transgenic plants has important theoretical and practical significances and can be used for cultivating and identifying varieties of the insect resistance plants required by agriculture and animal husbandry and treatment of the ecological environment.

Description

technical field [0001] The invention relates to a protein related to insect resistance, its coding gene and application. Background technique [0002] At present, the yield loss of crops due to insect infestation is very huge in the world. Traditionally, the most common way to control insect infestation is to apply a large number of chemical insecticides. While the use of chemical pesticides has achieved certain economic benefits, it has also brought about serious problems such as pesticide residues, environmental pollution, ecological damage, pest resistance and re-emergence. In order to meet the needs of sustainable agricultural development, environmental protection and human security, it is a common problem for mankind to vigorously strengthen the sustainable control of agricultural biological disasters, and it is also a severe challenge for scientific research in this field. Therefore, research and utilization of plant resistance genes to effectively control agricultur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 李传友杜敏敏蒋红玲王保赵久海
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI