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Fermentation tank for bacillus thuringiensis to generate unit cell

A technology of Bacillus thuringiensis and fermenter, which is applied in the direction of specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, biological material sampling method, etc., and can solve the problem that the mass transfer coefficient of the container cannot have a large range Improvement, long filtration and centrifugation process cycle, difficult temperature mixing, etc., to achieve good convective mixing effect, shorten post-processing time, and enhance the effect of convection and dispersion

Inactive Publication Date: 2011-08-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The air-lift fermenter has excellent performance when it is used for the fermentation of low solid content medium, but it cannot be used for high solid content medium fermentation, and the oxygen supply capacity of the reactor needs to be further improved
It has been reported that a number of baffle elements and reticulated air-lift fermenters are installed in the guide tube of the air-lift internal loop reactor, and the performance has been improved, but the enlargement of the fermenter is limited, and ventilation needs to be increased compared with mechanical stirring. If the external circulation pipe is configured to increase dissolved oxygen, the external pumping and circulation system will easily contaminate the fermentation broth with bacteria and damage the cells, and the high aspect ratio will make it difficult to mix the temperature, cells, proteins, etc. precipitation is also difficult
[0005] There are problems with the aeration device used in the traditional general-purpose fermenter: the diameter of the bubbles increases with the increase of the ventilation rate, and the rising speed of the bubbles also increases; the degree of crushing of the bubbles does not increase with the increase of the stirring speed. Large, it can only be maintained at the same order of magnitude, increasing the ventilation rate and increasing the stirring power will not only fail to greatly improve the mass transfer coefficient of the container, but will lead to a large amount of energy consumption and an increase in product cost.
The current fermentation tanks are generally round bottom, the tank body does not adopt multi-stage cooling, and there is no segmental discharge, so it is impossible to cool the fermentation liquid in stages to promote precipitation, and discharge the materials according to their different concentrations, but discharge from the bottom of the tank The valve discharges the material together, the filtration and centrifugation process cycle is long, and the energy consumption is also large

Method used

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  • Fermentation tank for bacillus thuringiensis to generate unit cell
  • Fermentation tank for bacillus thuringiensis to generate unit cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Strain: 8010 was used as the fermentation strain to ferment and produce Bt.

[0023] Equipment: The traditional 1.5L mechanically ventilated stirring tank is used for culture feed, inoculation to the stage of cell production; for the fermentation stage of Bacillus thuringiensis cell production stage, a 1.5L such as figure 1 A fermenter at the stage of Bacillus thuringiensis cell production is shown, with a height-to-diameter ratio of 2:1, a 70° conical bottom, an elliptical head on the top of the tank, and two jet mixers installed above the conical bottom of the tank. There is a cooling zone at the bottom of the cone and 1 / 3 and 2 / 3 of the height above it, and a safety valve, a vacuum valve, a gas-liquid splitter interface, and an inspection hole are installed on the top of the tank. Outlets, one outlet is set at 1 / 4 and 1 / 2 above the bottom of the cone.

[0024] Medium formula: 1% corn starch, 1.3% soybean meal, 1% peanut cake powder, 1% corn steep liquor, 0.1% yeast ...

Embodiment 2

[0031] As in Example 1, a traditional 2.5L mechanically ventilated stirring tank is used for feeding and inoculating the culture medium to the stage of cell production; for the fermentation stage of Bacillus thuringiensis cell production stage, a 2.5L such as figure 1 A fermenter at the cell-producing stage of Bacillus thuringiensis shown has a height-to-diameter ratio of 2.5:1, a conical bottom, and an elliptical head on the top of the tank. Four jet mixers are installed above the conical bottom of the tank, and the conical bottom of the tank is 1 / 3 and 3 / 5 of the top of the tank are equipped with a cooling zone, a safety valve, a vacuum valve, a vapor-liquid splitter interface, and an inspection hole are installed on the top of the tank, and a discharge valve is installed at the bottom of the tank body. There is a discharge port at the 1 / 4 and 1 / 2 above the mouth and the bottom of the cone.

[0032] The effect of product is with embodiment 1, and benefit is slightly higher t...

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Abstract

The invention provides a fermentation tank for bacillus thuringiensis to generate unit cells, the height-diameter ratio is from 2:1 to 3:1, and the cone bottom and the tank top are provided with ellipse heads; 2-4 jet flow mixers are arranged above the cone bottom of a tank body, the cone bottom of the tank body is provide with one cooling belt, and two cooling belts are arranged at the 1 / 3-2 / 3 above the cone bottom of the tank body; the tank top is provided with a safety valve, a vacuum valve, a vapor-liquid shunt interface and an overhaul inlet, the cone bottom of the tank body is provided with one discharge hole, and one discharge hole is respectively arranged under the two cooling belts at the upper part of the tank body; and the fermentation tank is matched with a normal fermentation tank to be specially used for performing the post-fermentation on fermentation liquid when germ is cultivated to form at first in the normal fermentation tank. Therefore, the energy consumption caused by the fermentation can be reduced, the yield of the germ and crystalloid can be improved, and the fermentation tank is good for separation of the crystalloid and the germ.

Description

technical field [0001] The invention belongs to the field of fermentation equipment, in particular to fermentation equipment at the spore-producing and crystal stages of Bacillus thuringiensis (Bt) fermentation, that is, a Bacillus thuringiensis spore-producing fermentation tank. Background technique [0002] Generally, oxygen is difficult to dissolve in water. When the fermented liquid contains various other components, the solubility of oxygen is lower than that in pure water, and the higher the concentration of the fermented liquid, the slower the dissolved oxygen rate, while the microbial respiration and metabolic activities Oxygen required in fermentation must be dissolved in the fermentation broth before it can be directly utilized. Insufficient oxygen supply and short interruption of oxygen supply will cause serious irreversible damage to fermentation. Therefore, it is of great significance to effectively and rationally design the fermenter. In recent years, in order...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/04C12M1/00
CPCC12M23/02C12M41/22C12M33/22C12M27/02
Inventor 吴丽云关雄
Owner FUJIAN AGRI & FORESTRY UNIV