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95results about How to "Overcome weakness" patented technology

Construction method for next-generation sequencing library based on single-stranded connector and application thereof

The invention discloses a construction method for a next-generation sequencing library based on a single-stranded connector and application thereof. The method comprises the following steps: (1) carrying out denaturation on a double-stranded DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) / DNA heterozygote segment to generate single-stranded DNA; (2) connecting one single-stranded connector with a 3' end of the single-stranded DNA; (3) extending the single-stranded DNA connected with the single-stranded connector by utilizing DNA polymerase to generate double-stranded DNA; (4) connectingthe other end of the double-stranded DNA with a T connector or a Tn5 label connector; (5) carrying out PCR (Polymerase Chain Reaction) amplification on the double-stranded DNA with two ends connectedwith the connectors to form a DNA library capable of sequencing of a next-generation sequencing technology. The method disclosed by the invention can be used for constructing the next-generation sequencing library and determining a DNA sequence and also can be used for identifying a chromatin open region, carrying out gene expression detection, carrying out trace nucleic acid amplification and thelike; the method is a novel method which belongs to the field of nucleic acid detection and analysis and has various functions and wide application value.
Owner:SOUTHEAST UNIV

Rotary lifting construction method for cylindrical reticulated shell

The invention relates to a rotary lifting construction method for a cylindrical reticulated shell, and relates to a building construction method. The rotary lifting construction method comprises the following steps of: (1) splicing the cylindrical reticulated shell (1) on the ground; (2) supporting one end of the spliced cylindrical reticulated shell (1) by using a rotating hinge (3), and supporting a spliced extension operating surface (1-1) at the other end of the spliced cylindrical reticulated shell (1) by using a temporary support (4); (3) lifting the cylindrical reticulated shell (1) by using a lifter, so that the cylindrical reticulated shell (1) rotates around the rotating hinge (3), and continuing to splice the cylindrical reticulated shell (1) on the spliced extension operating surface (1-1); and (4) performing the lifting process and the splicing process alternately, so that the cylindrical reticulated shell (1) extends along the direction of an arc line until the cylindrical reticulated shell (1) reaches a designed position. By the rotary lifting construction method, the key problem of the forming of a cylindrical surface in the installation construction process of a cylindrical reticulated shell structure is solved, and an installation construction method without a support is provided.
Owner:WUHU TIANHANG TECH GRP

Evaluation method and device for system technology maturity

InactiveCN103391317ASolve the technical problem of duplicate storageReduce storageTransmissionSpecial data processing applicationsEvaluation resultMulti dimensional
The invention relates to an evaluation method and device for the system technology maturity. The method comprises the steps that 1) administrators set and store following contents, including system technology maturity evaluation levels and level details and system work breakdown structures and key technology items, in a database in advance; 2) experts evaluate the system technology maturity of the system key technology items and store the system technology maturity in the database; 3) evaluation data of all experts are processed comprehensively based on weighting and short plate threshold algorithms to obtain evaluation results of the system technology maturity. According to the evaluation method and device, the method that only one copy of the technology maturity evaluation level detail data is stored is used, the technological problem of repeated storage of a great number of data is solved through a multi-dimensional extension data storage and processing method, and evaluation of a plurality of technology items of significant complex systems by a plurality of experts and automatic data statistic analysis are achieved. The evaluation method and device have the advantages that the data storage and processing efficiency is improved, evaluation and organization work is convenient and flexible, evaluation costs are reduced, and the like.
Owner:XIAMEN UNIV

Device and method for in situ measurement of energy distribution of focused laser faculae

The invention relates to a device and method for in situ measurement of energy distribution of focused laser faculae. The device is fixed on a high-precision translation stage by a bracket, wherein a bore for fixing a bore part is formed on a desktop of the bracket; a lens of a microscope is vertical to the bore part; a collecting lens is arranged under the bore of the desktop; the collecting lens is connected to a detector through an optical fiber; the detector is used for acquiring a transmitted laser signal and inputting the laser signal to a data processor; and an energy distribution chart of the laser faculae is acquired after the measured data are processed by the data processor. The measuring method comprises the following steps: confirming the bore position by utilizing the microscope and focusing the lasers to the bore position; adjusting and setting an exposure time of the detector; scanning and exposing according to a set step width; and supplying the energy distribution chart of the laser faculae by the data processor. According to the device and method, the accurate measurement for the energy distribution of the focused laser faculae is realized by utilizing the bore part to scan the laser faculae and coupling the acquired laser signal to the high-sensitivity detector with a wide dynamic response scope.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA

Symmetrical ternary string represented voice perception Hash sequence constructing and authenticating method

InactiveCN104134443AOvercome weaknessPerceptual hash digest is strongSpeech analysisAlgorithmVoice communication
The invention discloses a symmetrical ternary string represented voice perception Hash sequence constructing and authenticating method. The method comprises the steps that firstly, overall discrete wavelet transforming (DWT) is carried out on voice signals produced after preprocessing and intensity-loudness transformation (ILT); secondly, non-overlapping partitioning is carried out on the low-frequency part of the voice signals produced after DWT, and short-time logarithm energy of blocks is calculated to obtain the signal frequency-domain features; lastly, a final ternary perception Hash sequence is generated based on the time domain spectrum flux features (SFF) of the voice signals, and the voice frequency content is quickly authenticated through Hash matching. The symmetrical ternary string representation of the perception Hash abstract is superior to that of the binary form, the common voice content is operated between the robustness and the difference in a balanced mode, the time complexity of the algorithm is low, efficiency and the abstraction are high, precise manipulation detecting and positioning can be achieved, and the method can be used for authenticating a mobile voice communication terminal with bandwidth resources limited in real time.
Owner:LANZHOU UNIVERSITY OF TECHNOLOGY

Insect-proof durable filament for plastic woven bags

The invention discloses an insect-proof durable filament for plastic woven bags. The insect-proof durable filament is characterized by comprising, by weight, 100 parts of polypropylene, 0.1-0.5 part of pyrethroid, 1-1.8 parts of crosslinking agents, 80 parts of filled plastic master batch, 2.8-3.0 parts of acrylic acid dimethyl amino ethyl ester, 0.25-0.45 part of nano-graphite, 0.4-0.85 part of antioxidants, 1.1-1.5 parts of silane coupling agents, 2-8 parts of toner, 0.3-0.8 part of butyltin oxide and 0.2-0.3 part of polypropylene wax. The insect-proof durable filament for the plastic woven bags has the advantages that the insect-proof durable filament comprises the ultrafine talc powder filled master batch, accordingly, problems of weakness, old appearance and the like of flat filaments can be effectively solved, and the appearance quality and the flexibility of the insect-proof durable filament which is a flat filament can be greatly improved; the insect-proof durable filament additionally comprises the pyrethroid, accordingly, the problem that plastic flat filaments are vulnerable to insect bite and damage can be effectively solved, and the application range of the insect-proof durable filament can be further expanded.
Owner:TIANJIN XUHUIHENGYUAN PLASTIC PACKAGING CORP
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