Device for automatically regulating and controlling oxidation-reduction potential and application thereof

A potentiometric and automatic technology, applied in biochemical cleaning devices, enzymology/microbiology devices, specific-purpose bioreactors/fermenters, etc., can solve problems such as high knowledge and experience requirements, large fluctuations, and high operating intensity , to optimize the production performance of the strain, increase the fermentation output, and improve the synergistic effect

Inactive Publication Date: 2011-08-17
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, these regulation processes are based on the ORP level of online monitoring, and are regulated by manual control based on experience. The operation intensity is high, the fluctuation is large, and there is a certain hysteresis, and the knowledge and experience of the operator are very demanding. high

Method used

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  • Device for automatically regulating and controlling oxidation-reduction potential and application thereof
  • Device for automatically regulating and controlling oxidation-reduction potential and application thereof
  • Device for automatically regulating and controlling oxidation-reduction potential and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1. A device for automatically regulating oxidation-reduction potential and its application method

[0051] 1. A device that automatically regulates the redox potential

[0052] Such as figure 1 As shown, a device for automatically regulating the oxidation-reduction potential includes a fermentation tank 8, an ORP electrode 9 arranged in a fermentation tank 8, a signal normalization converter 10, an I / O module 11, a central processing unit 1, and an I / O module 3. Electric signal control pulse valve 5, peristaltic pump 4.

[0053] Fermentation tank 8 is a place for fermentation. The ORP electrode 9 is used to measure the oxidation-reduction potential in the fermenter 8 and can generate an ORP electrode signal. Signal standardization converter 10 and I / O module 11 are arranged between ORP electrode 9 and central processing unit 1, wherein, signal standardization converter 10 can convert the ORP electrode signal of ORP electrode 9 to the standard signal of 4-2...

Embodiment 2

[0076] Embodiment 2, using the device online monitoring of the automatic regulation of redox potential of embodiment 1

[0077] As described in 1 of step 2 of Example 1, the device for automatically regulating the oxidation-reduction potential of the present invention can monitor and automatically record the ORP change of the measured system on-line, collect data, and the data interval can be set arbitrarily by the user. The time interval is It can be greater than 1 second. The collected data is recorded by the system to generate an automatic report, which can be opened in excel format for users to call.

[0078] This example takes the fermentation of Klebsiella (Klebsiella) as an example. This system is used to detect and record two different fermentation conditions of Klebsiella oxytoca (Klebsiella oxytoca): Klebsiella oxytoca ATCC 43165 (preserved by the American ATCC strain bank, preserved No. 43165; China Industrial Microorganism Culture Collection Management Center CICC...

Embodiment 3

[0085] Embodiment 3: Utilize the device for automatically regulating the redox potential of embodiment 1 to control the flow rate of air, oxygen and / or nitrogen to automatically regulate ORP

[0086] As described in 2 of step 2 of Example 1, the device for automatically regulating the redox potential of the present invention can adjust the air / nitrogen flow into the system, and use glycerol as a substrate for Klebsiella oxytoca ATCC 43165 The fermentation process that produces 1,3-propanediol is regulated.

[0087] Seed medium ( / L): Dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 4.45g, potassium dihydrogen phosphate (KH 2 PO 4 ) 1.3g, ammonium sulfate ((NH4) 2 SO 4 ) 3.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.2g, calcium carbonate (CaCO 3 ) 2.0g, yeast extract (Yeast extract) 1.0g, glucose 20g, trace element solution (Trace Elemental Solution, hereinafter referred to as TES) 2.0ml, iron solution (Fe 2+ solution) 2.0ml, pH 7.0.

[0088] Fermentation medium ( / L): ...

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Abstract

The invention discloses a device for automatically regulating and controlling oxidation-reduction potential and an application thereof. The device comprises at least one fermentation tank, an oxidation-reduction potential (ORP) electrode, a central processing unit, a gas flow controller and the like, wherein the ORP electrode is used for measuring the oxidation-reduction potential in the fermentation tank, the central processing unit is used for receiving a signal of the ORP electrode and comparing the signal of the ORP electrode with the signal value preset by a user or a signal spacing value to generate a control signal; and the gas flow controller is used for receiving the control signal of the central processing unit and controlling the flow speed of gas which leads to the fermentation tank according the control signal. The device systematically integrates processes of oxidation-reduction potential on-line monitoring, signal processing and feedback control and the like, can be used for optimizing the oxidation-reduction potential of a fermentation system to improve the fermentation yield, and also can be used for screening novel industrial production bacterial strains with good properties, and optimizing the production performance of the bacterial strains. Due to the development, the device also can be used for synchronizing the monitoring process of the oxidation-reduction potential with the automatic monitoring process of the other fermentation control, further the synergistic effect of the device is improved.

Description

technical field [0001] The invention relates to a device for automatically regulating oxidation-reduction potential and its application. Background technique [0002] Regardless of chemical reaction or biotransformation, it is necessary to control the conditions of the reaction system. Temperature, pressure, pH, concentration, etc. are common control conditions, and automatic monitoring and control has basically been realized (Table 1). These monitoring methods are applied to industrial production, which greatly improves production efficiency and reduces labor intensity of workers. However, many important environmental parameters have not yet achieved online monitoring and automatic control, and oxidation-reduction potential is one of them. Oxidation-reduction potential (also known as oxidation-reduction potential, oxidoreduction potential or redox potential or reduction potential, referred to as ORP) indicates the ability of a substance or system to give or obtain electron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36
Inventor 张延平王少华刘伟李寅
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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