Yeast extract powder for high-quality biological medium and preparation method thereof
A technology for extracting yeast powder and culture medium, which is applied in the field of yeast extract powder for high-quality biological media and its preparation, to achieve the goal of improving enzymatic hydrolysis efficiency and amino nitrogen yield, good desalination and impurity removal, and high-value comprehensive utilization Effect
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Embodiment 1
[0030] The fresh beer waste yeast Saccharomyces cerevisiae (Pearl River Brewery Co., Ltd.) that crosses 100 mesh sieves obtains 2.5 kg of beer waste yeast mud with a water content of 78.5% after being centrifuged at 3000 g for subsequent use;
[0031] Baker's yeast (Jiangmen Biotechnology Center) Saccharomycescerevisiae freshly cultivated by conventional methods obtains 2.5kg of baker's yeast mud 2.5kg containing water 77.5% after 3000g centrifugal washing;
[0032] Add 5kg 5℃ water to the mixing tank, add 0.05kg NaHCO 3 , stirred to dissolve completely, NaHCO 3 Add the solution to 2.5kg of beer waste yeast sludge, stir for 30 minutes, and centrifuge at 3000g to obtain 2.5kg of debittered beer waste yeast sludge for later use;
[0033] Put 2.5kg of debittered beer waste yeast mud and 2.5kg of fresh bread yeast mud into the reaction tank, add 10kg of water and stir well, then add 0.065kg of salt, 0.065kg of potassium chloride, 1.5g of magnesium chloride and wall-breaking enzym...
Embodiment 2
[0050] Freshly cultivated baker's yeast by conventional method obtains 5.0 kg of baker's yeast mud containing water 77.5% after 3000g centrifugal washing;
[0051] Put 5.0kg of bread yeast mud into the reaction tank, add 10kg of water and stir well, adjust the pH to 7.0 with hydrochloric acid and sodium hydroxide, then add 0.1kg of salt, 0.1kg of potassium chloride, 0.065kg of magnesium chloride and 6.75g of wall-breaking enzyme Stir well afterward, and incubate enzymatic autolysis at 50°C for 8 hours;
[0052] Raise the temperature of the yeast liquid that has been preliminarily enzymatically autolyzed to 55°C, add 20g of yeast extract enzyme, further enzymatically hydrolyze for 8 hours, then raise the temperature to 75°C, and keep it warm for 30 minutes to inactivate the enzyme;
[0053] The enzyme-inactivated yeast solution was subjected to solid-liquid separation, and the obtained supernatant was passed through an ultrafiltration membrane with a molecular weight cut-off of...
Embodiment 3
[0063] The fresh beer waste yeast that crossed 100 mesh sieves was centrifuged at 3000g to obtain 2.0 kg of beer waste yeast mud with a water content of 78.5% for subsequent use;
[0064] Freshly cultivated baker's yeast obtained 3.0 kg of baker's yeast mud with a water content of 77.5% after 3000 g of centrifugal washing;
[0065] Add 4kg 5℃ water to the mixing tank, add 0.02kg NaHCO 3 , stirred to dissolve completely, NaHCO 3 The solution was added to 2.0kg of beer waste yeast sludge, stirred for 30 minutes, and centrifuged continuously at 3000g to obtain solid isolates for later use;
[0066] Put 2.0kg of beer waste yeast sludge for removal of impurities and debitterness and 3.0kg of fresh bread yeast sludge into the reaction tank, add 10kg of water and stir well, adjust the pH to 6.0 with hydrochloric acid, then add 0.1kg of salt and 0.1kg of potassium chloride in turn , 0.01kg of magnesium chloride and 15g of wall-breaking enzyme, stir well, and incubate at 55°C for 6 h...
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