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Preparation method of biological active montmorillonite powder and application of biological active montmorillonite powder serving as feed additive in feed

A technology of feed additive and montmorillonite powder, which is applied in the field of inorganic materials and microbial fermentation, can solve the problems of high cost of use, unclear effect, unstable product quality, etc., and achieve the effect of clear use effect and stable product quality

Active Publication Date: 2011-08-24
HANGZHOU XIANGTIANGE BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The application of the above-mentioned montmorillonite powder and microbial feed additives in feed still has the disadvantages of insufficient clear effect, unstable product quality, large dosage, and high use cost.

Method used

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  • Preparation method of biological active montmorillonite powder and application of biological active montmorillonite powder serving as feed additive in feed
  • Preparation method of biological active montmorillonite powder and application of biological active montmorillonite powder serving as feed additive in feed
  • Preparation method of biological active montmorillonite powder and application of biological active montmorillonite powder serving as feed additive in feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Add 300 mesh pharmaceutical-grade montmorillonite powder to the fermentation medium to ferment Bacillus subtilis to prepare bioactive montmorillonite powder containing Bacillus subtilis.

[0036] The specific steps are:

[0037] (1) Activate the freeze-dried bacteria stored at 4°C or the liquid bacteria stored at -80°C. Bacillus subtilis ACCC10619 was streaked on the pre-prepared LB solid culture dish, and then placed in a biochemical incubator set at 37°C for 18 hours.

[0038] (2) Preparation of primary seed solution. Pick a typical uniform single colony from the activated culture dish and inoculate it into the prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 37°C, and the rotation speed at 150rpm, and cultivate for 18 hours . Obtain first-class seed liquid.

[0039] (3) Preparation of secondary seed solution. Firstly, the air filter, air duct and fermenter of the fermentation system for making t...

Embodiment 2

[0047] Application of biologically active montmorillonite powder added to feed to prevent diarrhea and promote growth in weaned piglets

[0048] Gastrointestinal stress indigestion and diarrhea in piglets caused by early weaning of piglets are old problems that have plagued the pig industry for many years. In order to solve the problem of early diarrhea in piglets, the method of adding super large doses of zinc oxide and antibiotics to feed is widely used at home and abroad to reduce the diarrhea rate of piglets from the mechanism of astringent intestines, sterilization or inhibition of intestinal pathogenic microorganisms. However, side effects and drug residues have attracted more and more attention. Montmorillonite and probiotics are new methods developed to prevent and treat diarrhea in weaned piglets. There are no reports of toxic and side effects in these two methods. The bioactive montmorillonite prepared in Example 1 of the present invention is compared with common mon...

Embodiment 3

[0075] Add 200 meshes of pharmaceutical grade montmorillonite powder to the fermentation medium to ferment Bacillus licheniformis to prepare bioactive montmorillonite powder containing Bacillus licheniformis.

[0076] The specific steps are:

[0077](1) Activate the freeze-dried bacteria stored at 4°C or the liquid bacteria stored at -80°C. Bacillus licheniformis CICC23584 was streaked on the LB solid culture dish prepared in advance, and then placed in a biochemical incubator set at 35°C for 24 hours.

[0078] (2) Preparation of primary seed solution. Pick a typical uniform single colony from the activated culture dish and inoculate it into the LB liquid sterile medium prepared in advance, then place it in a constant temperature shaking incubator, set the temperature at 32°C and rotate at 150rpm, and incubate for 22 hours . Obtain first-class seed liquid.

[0079] (3) Preparation of secondary seed solution. Firstly, the air filter, air duct and fermenter of the fermentat...

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Abstract

The invention relates to a preparation method of biological active montmorillonite powder and an application of the biological active montmorillonite powder serving as a feed additive in feed. The preparation method comprises the following steps: adding the montmorillonite powder in a fermentation medium of probiotic so as to carry out fermentation together with the probiotic; and arrying out sedimentation, centrifugation, drying and crushing on fermentation products, thus obtaining the biological active montmorillonite powder containing the probiotic and metabolic product of the probiotic. In the invention, the obtained biological active montmorillonite powder is mixed with a premixed material, a concentrated material, a complete feed or the raw material of the product at 5-50g in each ton of complete feed, after the biological active montmorillonite powder is fed to animals, the growth of the animals can be enhanced, diarrhea can be prevented, especially the growth of weaned pigs isenhanced and the diarrhea of the weaned pigs can be prevented.

Description

technical field [0001] The biologically active montmorillonite powder of the invention relates to the field of inorganic materials and microbial fermentation, in particular to the application of the biologically active montmorillonite powder as an animal feed additive in feed. Background technique [0002] Montmorillonite, also known as microcrystalline kaolinite, is a silicate clay mineral with a layered structure and flaky crystals. It is the main component of bentonite and belongs to the dioctahedral subgroup of the Smectite family. A layer of silicon-oxygen tetrahedral sheets connected by a common top layer and a layer of aluminum (magnesium) oxygen (oxygen) octahedral sheets connected by a common edge form a 2:1 type structure containing crystal water, which is the most variable crystal structure among clay minerals. One of the minerals, the test results of the slow scanning of the diffractometer show that it is a natural nanomaterial. Montmorillonite particles have a ...

Claims

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Application Information

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IPC IPC(8): A23K1/175A23K10/12A23K10/18A23K20/105A23K20/147A23K20/163A23K20/20A23K20/22A23K20/24A23K20/26A23K20/28A23K40/00A23K50/30A23K50/60
Inventor 黄海智邱晓力储卫华黄丽丽
Owner HANGZHOU XIANGTIANGE BIO TECH
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