Method for improving proliferation capability of mycoplasma gallisepticum in vaccine production

Abstract

The invention relates to a method for improving proliferation capability of mycoplasma gallisepticum in vaccine production, vaccines are prepared through the process steps of preparing seeds, preparing a culture medium of the mycoplasma gallisepticum, preparing vaccine-making bacterial liquid and preparing the inactivated vaccines, after improvement, the content of PPLO (pleuropneumonia-like organism) broth, porcine serum and glucose is increased, MEM (minimum essential medium) ingredients are removed, and the mixture ratio of the ingredients in the culture medium are more scientific and the nutritional value is higher after adjustment. The bacterial liquid concentration of a semi-finished product prepared by the method is as high as 1.0*1013CCU / ml-1.0*1014CCU / ml, and the bacterial liquiddoes not need to be concentrated and can be directly or indirectly used for preparing the inactivated vaccines after dilution, thereby not only simplifying the production process, but also reducing the production cost.

Description

technical field [0001] The invention relates to an improved method for improving the proliferation ability of mycoplasma gallisepticum, in particular to a cultivation method suitable for the production of mycoplasma gallisepticum vaccine and for improving the multiplication ability of mycoplasma gallisepticum. It belongs to the technical field of biomedicine. Background technique [0002] Mycoplasma gallisepticum is a chronic respiratory disease (CRD) in chickens and turkeys caused by Mycoplasma gallisepticum (MG). Clinically, the main manifestations are cough, runny nose, rales when breathing, and mouth breathing in severe cases. With the increase of chicken scale, the change of feeding method and the increase of stocking density, the incidence of the disease is getting higher and higher. According to statistics, after Mycoplasma gallisepticum infected chickens, the weak chick rate of chicks increased by about 10%, the laying rate of laying hens decreased by 10-20%, the b...

AI Technical Summary

Problems solved by technology

[0004] The Mycoplasma gallisepticum cultivated by the above-mentioned traditional medium and culture process has poor proliferation ability and low culture titer (low concentration of the culture solution), and the bacteria solution (semi-finished product) used for seedling production cannot be used directly in the production of inactivated vaccines. The finished vaccine can only be prepared after a certain amount of concentration, but the concentration operation of the semi-finished product is cumbersome and the loss in the concentration process is large, which will eventually affect the protective efficacy of the finished vaccine and increase the production cost of the vaccine