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Oyster protein peptide and zinc chelate and method for preparing same

A technology of chelate and protease hydrolyzate, applied in the field of oyster protein peptide zinc chelate and its preparation, can solve the problems of low absorption and utilization rate, large side effects, etc., and achieves lowering blood pressure, improving absorption and utilization rate, and safe preparation process. Effect

Inactive Publication Date: 2011-09-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention uses enzymatic hydrolysis technology to prepare oyster protein bioactive peptides, and provides a preparation method of oyster protein peptide zinc chelate to overcome the defects of large side effects and low absorption and utilization rate of traditional zinc supplement products, so that the chelated zinc can Maintain a soluble state in the intestinal tract, so as to develop a natural, safe and efficient biological zinc supplement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 1.0kg of oyster meat, beating and crushing, add flavor protease 6g, adjust the enzymolysis temperature to 42°C, enzymolysis time 6h, and pH 7.0 for enzymolysis, after the end of enzymolysis, inactivate the enzyme at 90°C for 10min, and use ultrafiltration equipment ( The molecular weight of the ultrafiltration membrane is 3000Da) for ultrafiltration to obtain relatively high-purity oyster protein bioactive peptides, which are cooled to room temperature for subsequent use.

[0018] Oyster protein bioactive peptide solution, according to bioactive peptide and ZnSO 4 (in Zn 2+ A mass ratio of 5:1, a reaction pH of 6.0, a reaction temperature of 45° C., and a reaction time of 30 min were used for the chelation reaction to obtain oyster protein peptide zinc chelate.

Embodiment 2

[0020] Take 1.0kg of oyster meat, beating and crushing, add flavor protease 8g, adjust the enzymolysis temperature to 45°C, enzymolysis time 5h, and pH 7.2 for enzymolysis, after the end of enzymolysis, inactivate the enzyme at 70°C for 20min, and use ultrafiltration equipment ( The molecular weight of the ultrafiltration membrane is 3000Da) for ultrafiltration to obtain relatively high-purity oyster protein bioactive peptides, which are cooled to room temperature for subsequent use.

[0021] Oyster protein bioactive peptide solution, according to bioactive peptide and ZnSO 4 (in Zn 2+ A mass ratio of 4:1, a reaction pH of 6.3, a reaction temperature of 55° C., and a reaction time of 20 min were used for the chelation reaction to obtain oyster protein peptide zinc chelate.

Embodiment 3

[0023] Take 1.0kg of oyster meat, beating and crushing, add flavor protease 10g, adjust the enzymolysis temperature to 45°C, enzymolysis time 5h, pH 7.5 for enzymolysis, after the end of enzymolysis, 85°C to inactivate 15mm of enzyme, use ultrafiltration equipment ( The molecular weight of the ultrafiltration membrane is 3000Da) for ultrafiltration to obtain relatively high-purity oyster protein bioactive peptides, which are cooled to room temperature for subsequent use.

[0024] Oyster protein bioactive peptide solution, according to bioactive peptide and ZnSO 4 (in Zn 2+ A mass ratio of 3:1, a reaction pH of 6.5, a reaction temperature of 60° C., and a reaction time of 15 minutes were used for the chelation reaction to obtain oyster protein peptide zinc chelate.

[0025] The above oyster raw materials were purchased from Qingdao farmers market.

[0026] The flavor protease mentioned above was purchased from Novozymes Biological Co., Ltd.

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Abstract

The invention relates to an oyster protein peptide and zinc chelate and a method for preparing the same. The method comprises the following steps: pulping and grinding oyster meat, carrying out enzymolysis on the oyster meat by using flavor protease, and chelating the product of enzymolysis and Zn<2+> compound under a certain condition to obtain the oyster protein peptide and zinc chelate. The oyster protein peptide and zinc chelate can be used as zinc supplementing agent and gradually dissolved under the intestinal weak-alkalinity condition to gradually release Zn<2+>ions so that the absorption and utilization rates of the Zn<2+>ions are effectively improved. Due to the existence of bioactive peptide, the oyster protein peptide and zinc chelate has the physiological functions of resisting oxidation, regulating immune and lowering blood pressure. The preparation process of the oyster protein peptide and zinc chelate is safe and free from pollution. The by-products from processing of the oyster products can be used as raw materials, thus the demand on high-value usage of aquatic products can be met, and shellfish resources in coastal areas of China can be fully utilized, the healthy and sustainable development of aquaculture can be promoted, and important social and economic significances are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an oyster protein peptide zinc chelate and a preparation method thereof. Background technique [0002] Zinc is an essential trace element for the human body, and zinc deficiency can cause various diseases. Zinc is widely involved in the metabolism of nucleic acids and proteins, thus affecting the growth, division and differentiation of various cells, especially the basic life processes such as DNA replication and RNA transcription. In addition, zinc can speed up cell division and keep cell metabolism at a high level. However, phosphate and oxalic acid in food interfere with the absorption of zinc, and the absorption and utilization rate of inorganic zinc and organic zinc are also low. Therefore, finding a safe and effective zinc supplement product is of great significance to human health. [0003] Studies have proved that amino acids and small peptides can promote zinc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K4/12C07K1/113C12P21/06A61P3/02A61P39/06A61P37/02A61P9/12
Inventor 刘尊英陈达张璟
Owner OCEAN UNIV OF CHINA
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