Fluorescence analysis preparation, method for preparing same and application thereof
A fluorescence analysis and reagent technology, applied in fluorescence/phosphorescence, material excitation analysis, chemical instruments and methods, etc., can solve the problems of application limitation, poor water solubility, etc., and achieve the effect of excellent water solubility
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Embodiment 1
[0029] Embodiment 1, the preparation of fluorescence analysis reagent (structural formula is shown in formula (IV))
[0030]
[0031] Dissolve 100mg (0.37mmol) of dansyl chloride (DNS-Cl) in 1mL of dichloromethane to prepare a dichloromethane solution of dansyl chloride; mix 200mg (0.34mmol) of the compound shown in formula (V) with 50mg (0.36mmol ) Anhydrous potassium carbonate was added in 50mL of dichloromethane (in this reaction system, the mole fraction ratio of the compound shown in formula (V), dansyl chloride and potassium carbonate was 1: 1.1: 1.1), in an argon atmosphere Under protection, stir vigorously and add the dichloromethane solution of dansyl chloride prepared above dropwise; after the dropwise addition, stir at 25°C for 12 hours, then filter the reaction system, collect and concentrate the filtrate, and obtain the purple-red The crude product was ultrasonically washed several times with anhydrous ether to remove unreacted dansyl chloride; the residual sol...
Embodiment 2
[0032] Fluorescence analysis reagent shown in embodiment 2, formula (IV) is to the detection of hypochlorous acid
[0033] Add 20 μL of the mother solution of the fluorescent analysis reagent shown in formula (IV) (aqueous solution with a concentration of 1 mM) into a 10 mL test tube, and then add 5 mL of Na 2 HPO 4 -NaH 2 PO 4Buffer solution (pH=7.4) and 4 mL of secondary deionized water, and finally add different amounts of sodium hypochlorite standard solution (1 mM aqueous solution) and dilute to 10 mL with secondary deionized water, shake and react at room temperature for 20 min. Measure the fluorescence spectrum of the reaction system, the excitation and emission wavelengths are respectively 370nm and 501nm ( figure 2 ).
[0034] like figure 2 As shown, as the concentration of sodium hypochlorite increases, the fluorescence intensity of the reaction system at 501 nm and 585 nm increases gradually. In this embodiment, the fluorescence response at 501 nm is selecte...
Embodiment 3
[0035] Embodiment 3, the fluorescence analysis reagent shown in formula (IV) is used as the selectivity research of common reactive oxygen species as the fluorescence analysis reagent
[0036] Add 20 μL of the mother solution of the fluorescent analysis reagent shown in formula (IV) (aqueous solution with a concentration of 1 mM) into a 10 mL test tube, and then add 5 mL of Na 2 HPO 4 -NaH 2 PO 4 Buffer solution (pH=7.4) and 4 mL of secondary deionized water, and finally sodium hypochlorite standard solution (final concentration: 20 μM) was added and the volume was adjusted to 10 mL with secondary deionized water, and the reaction was shaken at room temperature for 20 min. The fluorescence spectrum of the reaction system was measured, and the excitation and emission wavelengths were 370nm and 501nm, respectively.
[0037] Take 4 test tubes at the same time, change the sodium hypochlorite into the following four common active oxygen species, and repeat the above experiment. ...
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