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Tobacco leaf biochemical additive and preparation method and application thereof

An additive and biochemical technology, applied in biochemical equipment and methods, microorganism-based methods, applications, etc., can solve the problems of natural aroma, weakened layering, high cost of artificial and enzyme preparations, and reduced types of aroma substances. To achieve the effect of strong standardization and controllability, large-scale industrial production, and increase in the content of aroma components

Active Publication Date: 2013-02-13
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the aging time can be shortened by adding exogenous biological enzymes, because the variety of exogenous biological enzymes is too single, the types of aroma substances are reduced, and the natural and layered aroma is weakened
At the same time, this production method of adding exogenous biological enzymes to natural alcoholization, the cost of labor and enzyme preparation is relatively high, which makes the existing production method cycle too long, requiring a large amount of capital and warehouses, and the production cost is relatively high
How to overcome the above problems, there is no ideal solution in the prior art

Method used

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  • Tobacco leaf biochemical additive and preparation method and application thereof
  • Tobacco leaf biochemical additive and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] - preparation of culture medium

[0032] 1) Slant preservation medium (g / L, g / v): tryptone 10, beef extract 3, NaCl 5, agar 15, with 20% Na 2 CO 3 Adjust the pH to 8.5, and sterilize at 121oC for 20 minutes;

[0033] 2) Liquid seed medium (g / L, g / v): tryptone 10, beef extract 3, NaCl 5, with 20% Na 2 CO 3 Adjust the pH to 8.5, and sterilize at 121oC for 20 minutes;

[0034] 3) Liquid enzyme production medium (%): bran 1.5, soybean peptone 1.5, casein 1.0, CaCl 2 0. 1% , K 2 HPO 4 0. 1 % , KH 2 PO 4 0.125%, MgSO 4 · 7H 2 O, 0.05%, FeSO 4 0.0004%, pH 8.0. Sterilize at 121oC for 20 min.

[0035] ——Preparation of strain Van35 fermentation extract

[0036] like figure 1 As shown, it mainly includes steps such as seed cultivation, fermentation, centrifugation, and ammonium sulfate precipitation.

[0037] The specific process is as follows: First, 50 mL of liquid seed medium was divided into 250 mL Erlenmeyer flasks, inoculated with strain Van35, and cult...

Embodiment 3

[0039] ——Product characteristics and physical and chemical properties

[0040] This product is a light brown transparent clear liquid, and the physical and chemical properties of the product are shown in Table 1.

[0041] Table 1. Physicochemical properties of biochemical additives

[0042]

Embodiment 4

[0044] ——Tobacco leaf alcoholization experiment

[0045] Prepare biochemical additives, take an appropriate volume (5%-10%) of additives and evenly spray on the surface of 100g tobacco leaves (control the moisture content of tobacco leaves at 12-13%), mix them evenly as a treatment sample, and age them in the aging workshop for 8 months. Spray the same amount of deionized water directly on the surface of 100g tobacco leaves (to control the moisture content of tobacco leaves to 12-13%) as a control sample. Do 5 repetitions.

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Abstract

The invention discloses a tobacco leaf biochemical additive (Van-1) and its preparation method and application, belonging to the technical field of tobacco additives. The bacterial strain used in the present invention is classified as Bacillus pumilus Van35; the preservation number is CGMCC No.3411. The biochemical additive Van-1 can effectively promote changes in the content of macromolecular compounds in tobacco leaves after treatment of tobacco leaves for 8 months, such as the increase of total sugar and reducing sugar content, and the decrease of total nitrogen, nicotine, protein and cellulose content ; At the same time, the content of aroma components in the tobacco leaves increased, and the quality of the tobacco leaves was close to that of the tobacco leaves samples that had been naturally aged for 18 to 24 months. The biochemical additive Van-1 is helpful to the conversion of chemical components in tobacco leaves and the formation and accumulation of aroma components, which can effectively improve the aging effect of tobacco leaves and shorten the aging time.

Description

technical field [0001] The invention belongs to the technical field of cigarette additives, and more specifically, the invention relates to a biochemical additive. At the same time, the invention also relates to the preparation method of the additive and its application in the tobacco industry. Background technique [0002] Tobacco is a special economic crop for smoking, and its use value depends on its quality. Taste is an important indicator for evaluating the quality of tobacco leaves. High-quality tobacco leaves require pure aroma, sufficient aroma, mellow taste and comfortable aftertaste. It is a complex systematic project from planting tobacco leaves to rolling into cigarette products, during which a series of steps such as threshing and re-baking and tobacco leaf aging are required. Among them, threshing and re-baking is the basic method to solve the defects in the quality of harvested tobacco leaves. The first-cured tobacco (also known as raw tobacco) purchased by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/20C12P1/04C12R1/07
Inventor 段焰青者为王明锋杨金奎朱保昆蒋举兴张克勤
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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