Enterovirus 71 antigen detection test strip (colloidal gold method)

Abstract

The invention relates to the field of biomedicine, and specifically relates to an enterovirus 71 antigen detection test strip (colloidal gold method) and a preparation method and application thereof. Enterovirus 71 can cause hand-foot-and-mouth disease, which has largegeneration proportion of severe infections (viral encephalitis, meningomyelitis virus and pulmonary edema), and a high death rate reaching 10%-25%. The test strip of the invention is used for rapid diagnosis of EV(enterovirus)71 infection. A virus separation and an RT-PCR (reverse transcription-polymerase chain reaction) are methods first used for EV71 antigen detection, but are not suitable for primary clinic usage due to defects of difficult operation and high costs, etc. The invention overcomes the above insufficiencies and provides a reagent, which is highly demanded in clinic detection, simply operated, suitable for various medical disease control sections, and capable of detecting EV71 antigens in human oropharyngeal swabs, bubble liquid, serum or excrement, and also provides the preparation method and application thereof. A technical scheme is as follows: a specimen is dropped on a sample pad, and the EV71 antigen wherein combines with a gold-labeled EV71 polyclonal antibody in a gold-labeled pad and migrates along a chromatography membrane. A detected line captures colloidal gold particles to form a red line visible to naked eyes, so as to realize detection of the EV71 antigen.

Description

Technical field: [0001] The invention relates to the field of biomedicine, in particular to a test strip for detecting enterovirus type 71 antigen and a preparation method and application thereof. technical background: [0002] Enterovirus EV71 infection is a general term for diseases caused by human enterovirus 71 (EV71). The main clinical manifestation is hand, foot and mouth disease. Hand, foot and mouth disease is mostly outbreaks in infants and young children. Some children with EV71 infection manifest as Herpetic angina, viral encephalitis, viral meningitis, pulmonary edema, pulmonary hemorrhage, etc. in severe patients. The most common pathogens that cause hand, foot and mouth disease are Coxsackie virus A16 and enterovirus 71; the disease caused by enterovirus 71 infection has a larger proportion of severe infections and a high mortality rate, and severe cases die. The rate can reach 10%-25%, so the etiological diagnosis is of great significance. [0003] The genom...

AI Technical Summary

Problems solved by technology

However, its sensitivity depends on the matching of the virus carried by the patient and the primers used on the one hand, and is also greatly affected by the higher-order structure of the amplified fragment on the other hand.

There have been quite a lot of efforts to find primers and amplification conditions that are common to different genotypes, but the results are not satisfactory. The main reason is that the coding interval of each subtype of EV71 varies greatly, and often the difference of a few bases is as large as Can cause significant changes in amplification efficiency

In addition, RT-PCR itself has high requirements on the operating environment and operators, and is expensive, so it is difficult to use in the majority of grassroots units

At present, only a few units in the market have RT-PCR capabilities, and it is difficult to find universal primers, and the primers currently used are genotype specific

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