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A method for cultivating microalgae and a combined photobioreactor

A photobioreactor and combined technology, applied in the biological field, can solve the problems of low carbon dioxide efficiency, unfavorable absorption and fixation, large carbon dioxide bubbles, etc., and achieve the effects of improving the growth rate of microalgae and efficiently fixing carbon dioxide.

Inactive Publication Date: 2011-12-07
UNIV OF SCI & TECH BEIJING
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Problems solved by technology

At present, there are mainly carbon dioxide ventilation methods such as bubbling air supply and fiber membrane air supply, but they all have the disadvantages of large carbon dioxide bubbles (millimeter scale), short residence time in water (several seconds), and unfavorable absorption and fixation of carbon dioxide by microalgae. Therefore, how to reduce the diameter of aerated carbon dioxide bubbles and increase their residence time in water is the key to solving the low efficiency of microalgae in fixing carbon dioxide.

Method used

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  • A method for cultivating microalgae and a combined photobioreactor
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  • A method for cultivating microalgae and a combined photobioreactor

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Embodiment Construction

[0021] 1. Algal species selection and culture medium

[0022] The algal species used in the experiment of the present invention is a Chlorella USTB-01 ( Chlorella sp.), strain preservation number: cgmcc No1448, preservation date: August 25, 2005. The composition of the medium is as follows (1000 ml deionized water): MgSO 4 7 hours 2 O 1.0 g, Na 2 HPO 4 12H 2 O 2.0 g, KNO 3 3.0 g, CaCl 2 20 mg, EDTA-2Na 0.02 g, ferric ammonium citrate 0.01 g. The initial pH of this medium is about 6.5.

[0023] 2. Culture control conditions

[0024] The invention uses a micro-nano aerator to form micro-nano bubbles from carbon dioxide passed into a microalgae cultivation pipeline, and performs light autotrophic cultivation of chlorella USTB-01 in a photobioreactor combined with a fermenter and a cultivation pipeline. Prepare 30 liters of medium and sterilize it with a steam generator at 100°C for 1 hour. After cooling to 30°C, inoculate Chlorella USTB-01 to start the culture. The ...

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Abstract

The invention relates to a method for using a micro-nano aerator to form micro-nano bubbles from carbon dioxide passed into a microalgae cultivation pool so as to achieve efficient fixation of carbon dioxide and efficient cultivation of microalgae, which belongs to the field of biotechnology. In the microalgae cultivation process, the present invention uses a micro-nano aerator to fully mix the culture outflow from the fermenter with the carbon dioxide introduced into it to form carbon dioxide bubbles with a diameter of 30 to 50 microns, which then flow into the culture pipeline for sufficient photosynthesis Then return to the fermenter, so that the microalgae culture can circulate in the fermenter, the micro-nano aerator and the cultivation pipeline, so as to achieve the technology and method of efficiently fixing carbon dioxide and cultivating microalgae. The invention also discloses a combined photobioreactor applied to the above method. It is characterized in that the micro-nano aerator can form tiny carbon dioxide bubbles, which not only facilitates the dissolution of carbon dioxide in the water body, but also greatly increases the residence time of carbon dioxide in the water.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for using carbon dioxide in a microalgae culture to form micro-nano bubbles to achieve high-efficiency fixation of carbon dioxide and high-efficiency cultivation of microalgae, and to a combined photobiological reaction used in the method device. Background technique [0002] The global climate change and greenhouse effect caused by the increase of carbon dioxide concentration in the global atmosphere pose a serious threat to human survival and sustainable development. my country, as the country with the largest carbon dioxide emissions, must bear the obligation and responsibility to reduce emissions. As the simplest photosynthetic organism, microalgae have survived on the earth for more than two billion years. It is reported that more than 90% of the oxygen in the atmosphere is produced by microalgae, and they also play a major role in fixing carbon dioxide in t...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/36C12M1/34C12M1/04C12M1/02B01D53/84B01D53/62C12R1/89
CPCC12M21/02C12M41/34C12M23/06C12M31/02C12M23/58C12M41/26
Inventor 闫海贾璇许倩倩张可毅李慧梅周枫
Owner UNIV OF SCI & TECH BEIJING
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