A method for cultivating microalgae and a combined photobioreactor
A photobioreactor and combined technology, applied in the biological field, can solve the problems of low carbon dioxide efficiency, unfavorable absorption and fixation, large carbon dioxide bubbles, etc., and achieve the effects of improving the growth rate of microalgae and efficiently fixing carbon dioxide.
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[0021] 1. Algal species selection and culture medium
[0022] The algal species used in the experiment of the present invention is a Chlorella USTB-01 ( Chlorella sp.), strain preservation number: cgmcc No1448, preservation date: August 25, 2005. The composition of the medium is as follows (1000 ml deionized water): MgSO 4 7 hours 2 O 1.0 g, Na 2 HPO 4 12H 2 O 2.0 g, KNO 3 3.0 g, CaCl 2 20 mg, EDTA-2Na 0.02 g, ferric ammonium citrate 0.01 g. The initial pH of this medium is about 6.5.
[0023] 2. Culture control conditions
[0024] The invention uses a micro-nano aerator to form micro-nano bubbles from carbon dioxide passed into a microalgae cultivation pipeline, and performs light autotrophic cultivation of chlorella USTB-01 in a photobioreactor combined with a fermenter and a cultivation pipeline. Prepare 30 liters of medium and sterilize it with a steam generator at 100°C for 1 hour. After cooling to 30°C, inoculate Chlorella USTB-01 to start the culture. The ...
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