A dynamic three-dimensional microscopic observation device for biological living cells based on digital holography

Abstract

The invention discloses a digital holography-based three-dimensional micro-observation apparatus for cell dynamic of a living organism. According to the apparatus provided in the invention, a microscopic technology of digital holography is employed, so that dynamic three-dimensional microscopic observation without contact, damage and pretreatment on a cell of a living organism can be realized; a fiber optical path is utilized to realize object light illumination and an optical path portion of the object light employs a vertical type structure, so that it is convenient to carry out observationon the cell of the living organism; reference light utilizes a light beam turnover device to control an included angle between the reference light and the object light so as to realize off-axis digitholography; and an infinite distance correction microscopic object lens is employed to carry out pre-amplification and imaging on the cell; simultaneously, a relay lens is utilized to adjust an imaging position and a wavefront curvature of the object light, so that observation resolution is improved. According to the apparatus, layout structures of a plurality of optical elements are compact, flexible and stable thorough a platform; moreover, the apparatus can be applied to dynamic observation on the cell of the living organism for a long time.

Description

technical field [0001] The present invention relates to a three-dimensional microscopic observation system, more particularly, to a dynamic three-dimensional microscopic observation system of biological living cells based on digital holography. Background technique [0002] At present, the three-dimensional microscopic observation techniques of biological cells mainly include atomic force microscopy, laser confocal microscopy and digital holographic microscopy. Relying on digital holographic technology, digital holographic microscopy uses CCD, CMOS and other photoelectric image sensors to record holograms in digital form, and uses computer simulation to reproduce the optical diffraction process of reference light irradiating holograms to reconstruct object light, so as to obtain the amplitude of the object to be measured and phase information. [0003] In order to apply digital holography technology to three-dimensional microscopic observation of living cells, some technica...

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Problems solved by technology

[0003] In order to apply digital holography technology to three-dimensional microscopic observation of living cells, some technical problems need to be solved: (1) The imaging resolution of digital holography is limited by the performance indicators of image sensors (CCD, CMOS). The microscopic objective lens is pre-amplified to improve the resolution of the reproduced image, but at this time the reproduced object light is easily affected by the aberration and distortion caused by the microscopic objective lens, and corresponding compensation and correction must be carried out

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