A universal detection medium for Gram-negative bacteria

A technology for universal detection of Gram-negative bacteria, which is applied in the field of universal detection medium for Gram-negative bacteria, can solve the problems of inappropriate selectivity and achieve the effects of high selectivity, simple preparation, and improved work efficiency

Inactive Publication Date: 2011-12-21
南宁海关技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the selectivity of bile salts as a universal detection medium for Gram-negative bacteria is not suitable

Method used

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  • A universal detection medium for Gram-negative bacteria
  • A universal detection medium for Gram-negative bacteria
  • A universal detection medium for Gram-negative bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The formula of the culture medium of Example 1 is shown in Table 1.

[0013] Add 5.0g of peptone, 2.5g of yeast extract, 1.0g of glucose, and 15.0g of agar into 1000mL of distilled water, boil to dissolve, adjust the pH to 7.0±0.2, dispense into Erlenmeyer flasks, 200mL each, and autoclave at 121°C After 15 minutes, the basal medium was obtained. Accurately weigh 10.0 mg of vancomycin hydrochloride, dissolve it in 100 mL of distilled water (equivalent concentration is 100 mg / L) to obtain a stock solution of vancomycin hydrochloride, divide into test tubes, and store at -20°C for later use.

[0014] Take 1mL bacteria concentration is 10 3 CFU / mL of Escherichia coli, Salmonella, Shigella, Proteus vulgaris, Proteus mirabilis, Citrobacter freundii, Lactobacillus, Enterococcus faecium, Bacillus licheniformis, Staphylococcus aureus were added to 9cm plate each 4 dishes ( A total of 40 dishes), pour the basal medium that was heated and cooled to 45°C into 2 dishes, 15mL per ...

Embodiment 2

[0017] The method of Example 1 was repeated, except that the composition of the basal medium was different, as shown in Example 2 in Table 1. The basal medium consists of 7.0g peptone, 3.0g yeast extract, 1.0g glucose, 5.0g sodium chloride and 15.0g agar. The final concentration of vancomycin hydrochloride is still 0.5mg / L. The culture results are shown in the data of Example 2 in Table 2. The result was the same as in Example 1, all Gram-negative bacteria grew, and Gram-positive bacteria did not grow.

Embodiment 3

[0019] The method of Example 1 was repeated, except that the composition of the basal medium and the concentration of vancomycin hydrochloride were different, as shown in Example 3 in Table 1. The basal medium consists of 5.0g peptone, 3.0g yeast extract, 1.0g glucose, 5.0g sodium chloride and 15.0g agar. The vancomycin hydrochloride stock solution was added in an amount of 2 mL, and its final concentration was 1.0 mg / L. The culture results are shown in the data of Example 3 in Table 2. The result was the same as in Example 1, all Gram-negative bacteria grew, and Gram-positive bacteria did not grow.

[0020] Table 1 General medium formula for Gram-negative bacteria detection

[0021]

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Abstract

A kind of Gram-negative bacteria universal detection culture medium of the present invention is composed of: peptone 5.0g~7.0g, yeast extract 2.5g~3.0g, glucose 1.0g, sodium chloride 0g~5.0g, agar 15.0g, Distilled water 1000mL, vancomycin hydrochloride 0.5mg/L~1.0mg/L. The culture medium of the present invention has high selectivity, and can specifically and selectively cultivate Gram-negative bacteria; the preparation is simple, and vancomycin is used alone as a selection agent, and the simplest formula even only needs to add 0.5 mg/L to the plate counting agar ~1.0mg/L of vancomycin hydrochloride is enough; it is friendly to human and environment, the concentration of vancomycin used is not more than 1.0mg/L; the work efficiency is improved, and it can directly count the colonies of Gram-negative bacteria.

Description

technical field [0001] The invention belongs to the field of a general detection culture medium for Gram-negative bacteria. Background technique [0002] There are many groups of microorganisms in the aquaculture environment. These microorganisms interact in the water body, grow and decline with each other, affect the change of water quality, and determine the success or failure of the aquaculture; therefore, to study the dynamic changes of the microbial groups, types and quantities in the aquaculture water, It is of great significance to guide aquaculture and protect environmental ecology. [0003] The microorganisms in the aquaculture environment are mainly bacteria, and the bacteria are divided into Gram-negative and Gram-positive. These two types of bacteria have different sensitivities to disinfectants due to their different cell wall structures. Therefore, rapid Understanding the changes in the bacterial flora in the water can guide the correct disinfection of the wat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/04
Inventor 刘军义谢春罗兆飞李娟
Owner 南宁海关技术中心
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