Influenza A H1N1/Influenza A Virus Nucleic Acid Dual Fluorescent PCR Detection Kit

Abstract

The invention provides a double fluorescent quantitative PCR detection kit of type A H1N1 / type A influenza virus nucleic acid, comprising RNase inhibitor, RT-PCR reaction solution, enzyme mixed solution, type A H1N1 / type A influenza virus double reaction solution , a positive control and a negative control, wherein said A / H1N1 / influenza A double reaction solution includes the following components: Component (1): a pair of primers for detecting A / H1N1 influenza virus and a primer for detecting A Type H1N1 influenza virus probe composition; component (2): composed of a pair of primers for detecting influenza A virus and a probe for detecting influenza A virus; said enzyme mixture includes Taq enzyme and reverse transcriptase . The invention can detect influenza A H1N1 and influenza A virus at the same time, and solves the problem that the existing products can only detect one type of influenza virus in one tube reaction. The invention has the advantages of simple operation, strong repeatability, rapid and objective detection results, etc., and has great application prospects in the field of in vitro diagnosis of influenza virus.

Description

technical field [0001] The invention relates to a dual fluorescent PCR detection kit for simultaneously detecting influenza A H1N1 / influenza A virus nucleic acid, belonging to the field of biotechnology. Background technique [0002] Influenza is a seasonal disease. In temperate regions, influenza prevails throughout the winter. In tropical regions, influenza viruses exist throughout the year, and are more common in rainy seasons. Common influenza A, commonly known as cold, is an acute respiratory infectious disease caused by influenza A virus as the pathogen, and the main mode of transmission is air droplet transmission. The severity of influenza is related to the individual immune status. Generally, only about 50% of infected patients will develop typical clinical symptoms of influenza. Typical symptoms of influenza include sudden fever, dizziness, headache, myalgia, mild systemic symptoms, and may be accompanied by sore throat and cough, nasal congestion, runny nose, ch...

AI Technical Summary

Problems solved by technology

[0005]1) Virus isolation and culture have high technical requirements, are expensive, and time-consuming, and the positive rate of isolation varies from laboratory to laboratory, which cannot meet the requirements of virus epidemic at the same time. The need to handle a large number of samples, currently only used for experimental research

[0006]2) Serological methods cannot process samples with high throughput, and cannot accurately reflect whether they are currently infected or carry the virus

[0008] At present, most of the products adopt the detection method for a single virus. For the clinical detection of multiple viruses, it is necessary to perform multi-tube detection on one sample, which increases the detection Cost and complexity of operation

[0009] From the above, it can be seen that the commonly used virus isolation method is time-consuming, the serological diagnosis method has hysteresis, and the gene sequence determination method is also relatively complicated and cumbersome.

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