Anti-herpes simplex virus polypeptide and its application

A herpes simplex virus and herpes simplex technology, applied in antiviral agents, applications, peptides, etc., can solve problems such as virus resistance and poor curative effect, and achieve non-toxic and side effects, significant therapeutic effect, and obvious results

Active Publication Date: 2011-12-28
WUHAN MORE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the use of these antiviral drugs has created resistance in the virus, making many herpes treatments less effective

Method used

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  • Anti-herpes simplex virus polypeptide and its application
  • Anti-herpes simplex virus polypeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Preparation of anti-herpes simplex virus polypeptide gene

[0031] Total RNA was extracted from the scorpion tail glands of 40 Tibetan scorpions (Scorpops tibetanus) with Trizol reagent (Invitrogen), and the extraction method was referred to the instructions of the Trizol kit. The Poly A Tract mRNA isolation system (Promega, USA) was used to purify the mRNA, and the Superscript Plasmid System cDNA library construction kit (Gibco / BRL) kit was used to construct the cDNA library of Tibetan lute scorpion venom tissue. The cDNA was cloned into pSPORT1 vector and transformed into E. coli DH5α. Randomly select 2000 clones from the constructed Tibetan piper venom cDNA library, and perform sequence analysis on the sequencing results, using CLUSTAL X 1.8 (Thompson et al., 1997) and PC / GENE (Intelligenetics Inc., Switzerland) software for Homology comparison and signal peptide cleavage site prediction obtained a cDNA gene encoding anti-herpes simplex virus active po...

Embodiment 2

[0032] Example 2: Structural analysis of AHSVP1 polypeptide and its homologous amphipathic polypeptide

[0033] According to the mature peptide sequence FIRKIARLLKRIF of AHSVP1 shown in SEQ ID NO: 1, that is, Phe Ile Arg Lys Ile Ala Arg Leu Leu Lys Arg Ile Phe, the online NPSserver [DSC method (Discrimination of protein Secondary structure Class)] was used to perform binary analysis on it. Secondary structure prediction, and use the software AHTHEPROT 2000 to draw its secondary structure image, see the results figure 2 . The secondary structure diagram shows that AHSVP1 contains 100% α-Helix structure, has a typical amphiphilic α-Helix structure, and contains a large number of basic residues (Arg and Lys) with net positive charges. Then, a large number of point mutations and deletions were performed on the AHSVP1 polypeptide sequence according to the AHSVP1 helical diagram, and the sequences are shown in Table 1.

[0034] Table 1: Anti-herpes simplex virus polypeptide AHSVP...

Embodiment 3

[0037] Example 3 Chemical Synthesis of AHSVP1 Polypeptide and Amphipathic Polypeptide with Homologous Structure

[0038] Artificially synthesized according to the amino acid sequence of AHSVP1 (Phe Ile Arg Lys Ile Ala Arg Leu Leu Lys Arg Ile Phe) and its homologous amphipathic polypeptide. The high-purity AHSVP1 polypeptide and its homologous amphipathic polypeptide were obtained by solid-phase chemical synthesis. The purity test of the prepared AHSVP1 polypeptide was carried out, and the results are shown in image 3 and Figure 4 . The detection results of AHSVP1 homologous structure polypeptides are not shown.

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PUM

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Abstract

The invention relates to the technical field of biology, and discloses anti-herpes simplex virus polypeptide and its application. Tests show that the anti-herpes simplex virus polypeptide has a high efficiency inhibition effect to the herpes simplex virus propagation, and the anti-herpes simplex virus polypeptide is used for producing the medicines for treating and preventing diseases related to herpes simplex. The invention provides a medicinal preparation which takes the anti-herpes simplex virus polypeptide as the effective component for treating the herpes caused by the herpes simplex virus, and has a obvious effectiveness for treating herpes, the effective rate for treating herpes simplex, labial herpes and genital herpes can reach to 100%. And the anti-herpes simplex virus polypeptide has low recurrence rate after heal and no toxic side effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an anti-herpes simplex virus polypeptide and its application. Background technique [0002] Herpes simplex virus (HSV) is a common human infectious disease virus that is widely distributed around the world and can cause gingivostomatitis, keratoconjunctivitis, encephalitis, reproductive system infection and neonatal morbidity. Infection can cause herpes simplex, cold sores, and genital herpes, which seriously affect human health. After the herpes simplex virus infects the host, it often establishes a latent infection in the nerve cells, and after activation, there will be asymptomatic detoxification. In the crowd, it enters the body through direct contact with the skin and mucous membranes or sexual contact, and the cycle repeats itself. [0003] The treatment of herpes virus includes general therapy, traditional Chinese medicine treatment and antiviral drug treatment, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K7/08C07K9/00C12N15/12A61K38/08A61K38/10A61K38/14A61P31/22
Inventor 李文鑫曹志贱吴英亮韩松
Owner WUHAN MORE BIOTECH
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