Botryococcus braunii magnetic separation method
A technology of botrytis brauneni and magnetic separation, applied in the direction of unicellular algae, ferrous oxides, nanotechnology, etc., can solve the problem of low yield and quality of botrytis braunenii cells, inability to regenerate and recycle the particles, and unproductive problems. Considering the coupling of oil extraction and separation medium recovery, the effects of easy scale-up, reduced production cost, and simple synthesis steps are achieved
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0054] Example one
[0055] The high-efficiency recovery method of magnetic nanoparticles used in the magnetic separation of Botryococcus braunii of the present invention is used to perform Fe 3 O 4 The steps for the recovery of nanoparticles are as follows:
[0056] (1) The prepared Fe 3 O 4 The nanoparticles are dispersed in deionized water to form a stable suspension, formulated into a magnetic separation medium with a concentration of 1% (w / v), and then placed in a refrigerator for refrigeration.
[0057] (2) Put the culture broth of Botryococcus braunii into a stirred reactor, add a magnetic separation medium at a speed of 200 rpm, the amount of magnetic separation medium is 0.02% (w / v), and the stirring time is 1.5 minutes; After separation, the supernatant was removed, and the polymer of Botryococcus brevifolia cells and magnetic medium was collected, and the recovery rate of Botryococcus brevifolia reached 99.8%;
[0058] (3) At room temperature, add 5 mL / g of polymer ethylene...
Example Embodiment
[0062] Example two
[0063] The high-efficiency recovery method of magnetic nanoparticles used in the magnetic separation of Botryococcus braunii of the present invention is used to perform Fe 3 O 4 The steps for the recovery of nanoparticles are as follows:
[0064] (1) The prepared Fe 3 O 4 The nanoparticles are dispersed in deionized water to form a stable suspension, formulated into a magnetic separation medium with a concentration of 0.5% (w / v), and then placed in a refrigerator for storage.
[0065] (2) Put the Botryococcus culture solution obtained by the culture into a stirred reactor, add a magnetic separation medium at a speed of 100 rpm, the amount of the magnetic separation medium is 0.01% (w / v), and the stirring time is 2 minutes; magnetic separation After removing the supernatant liquid, collecting the polymer of the separated Botryococcus braunii cells and the magnetic medium, the recovery rate of Botryococcus braunii reaches 99.5%;
[0066] (3) At room temperature, add...
Example Embodiment
[0070] Example three
[0071] The high-efficiency recovery method of magnetic nanoparticles used in the magnetic separation of Botryococcus braunii of the present invention is used to perform Fe 3 O 4 The steps for the recovery of nanoparticles are as follows:
[0072] (1) The prepared Fe 3 O 4 The nanoparticles are dispersed in deionized water to form a stable suspension, formulated into a magnetic separation medium with a concentration of 2% (w / v), and then placed in a refrigerator for refrigeration.
[0073] (2) Put the Botryococcus culture solution obtained by the culture into a stirred reactor, add a magnetic separation medium at 300 rpm, the amount of the magnetic separation medium is 0.03% (w / v), and the stirring time is 1 minute; magnetic separation After removing the supernatant liquid, collecting the polymer of the separated Botryococcus braunii cells and the magnetic medium, the recovery rate of Botryococcus brauni reaches 99.3%;
[0074] (3) At room temperature, add 8 mL / g...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap