Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Botryococcus braunii magnetic separation method

A technology of botrytis brauneni and magnetic separation, applied in the direction of unicellular algae, ferrous oxides, nanotechnology, etc., can solve the problem of low yield and quality of botrytis braunenii cells, inability to regenerate and recycle the particles, and unproductive problems. Considering the coupling of oil extraction and separation medium recovery, the effects of easy scale-up, reduced production cost, and simple synthesis steps are achieved

Inactive Publication Date: 2012-01-18
INST OF PROCESS ENG CHINESE ACAD OF SCI
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the magnetic nano-Fe used in it 3 o 4 The particles cannot be recycled and recycled, and it does not consider the coupling of oil extraction and separation medium recovery, resulting in low yield and quality of obtained S.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Botryococcus braunii magnetic separation method
  • Botryococcus braunii magnetic separation method
  • Botryococcus braunii magnetic separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Using the high-efficiency recovery method of magnetic nanoparticles for the magnetic separation of Staphylococcus branici of the present invention, Fe in the magnetic separation of Staphylococcal 3 o 4 The recovery of nanoparticles, the steps are as follows:

[0056] (1) Fe obtained by preparing 3 o 4 The nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 1% (w / v), and then put into a refrigerator for further storage.

[0057] (2) put the botrytis brannica culture solution obtained by cultivating into a stirred reactor, add a magnetic separation medium at a rotating speed of 200rpm, the consumption of the magnetic separation medium is 0.02% (w / v), and the stirring time is 1.5 minutes; After the separation, the supernatant was removed, and the separated botrytis brachyphytum cells and the polymer of the magnetic medium were collected, and the recovery rate of the botrytis brach...

Embodiment 2

[0063] Using the high-efficiency recovery method of magnetic nanoparticles for the magnetic separation of Staphylococcus branici of the present invention, Fe in the magnetic separation of Staphylococcal 3 o 4 The recovery of nanoparticles, the steps are as follows:

[0064] (1) Fe obtained by preparing 3 o 4 The nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 0.5% (w / v), and then put into a refrigerator for storage.

[0065](2) Put the botrytis culture solution obtained by cultivation into a stirring reactor, add a magnetic separation medium at a rotating speed of 100rpm, the consumption of the magnetic separation medium is 0.01% (w / v), and the stirring time is 2 minutes; Finally, the supernatant was removed, and the isolated botrytis brachyphytum cells and the polymer of the magnetic medium were collected, and the botrytis recovery rate reached 99.5%;

[0066] (3) At room temper...

Embodiment 3

[0071] Using the high-efficiency recovery method of magnetic nanoparticles for the magnetic separation of Staphylococcus branici of the present invention, Fe in the magnetic separation of Staphylococcal 3 o 4 The recovery of nanoparticles, the steps are as follows:

[0072] (1) Fe obtained by preparing 3 o 4 Nanoparticles are dispersed in deionized water to form a stable suspension, prepared into a magnetic separation medium with a concentration of 2% (w / v), and then put into a refrigerator for further storage.

[0073] (2) Put the botrytis culture solution obtained by cultivation into a stirred reactor, add a magnetic separation medium at a rotating speed of 300rpm, the consumption of the magnetic separation medium is 0.03% (w / v), and the stirring time is 1 minute; Finally, the supernatant was removed, and the isolated botrytis brachyphytum cells and the polymer of the magnetic medium were collected, and the botrytis recovery rate reached 99.3%;

[0074] (3) At room tempe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention dicloses a botryococcus braunii magnetic separation method for the regeneration cycle of a magnetic medium, which comprises the following steps: (1) dispersing Fe3O4 nano-particles to form a stable suspension; (2) adding the magnetic separation medium into a botryococcus braunii culture solution, stirring, performing magnetic separation to obtain a polymer of botryococcus braunii and the magnetic medium; (3) adding ethylene glycol dimethyl ether into the polymer for treatment for 2-10 min, adding n-hexane, stirring, and collecting residues; (4) washing the residues, adding hydrochloric acid for reaction, filtering after the reaction is finished, collecting the filtrate; (5) adjusting the pH of the filtrate to 0.8-1.5 by ammonia water, preparing Fe3O4 nano-particles with the filtrate as a raw material so as to realize the regeneration cycle of the magnetic medium. The method of the invention couples the oil extraction with the recovery of the separation medium, and realizes the recovery of the magnetic separation medium; the regeneration recovery of Fe3O4 nano-particles is realized by methods of hydrochloric acid dissolution and ammonia water recrystallization.

Description

technical field [0001] The invention belongs to the field of separation of microalgae, in particular, the invention relates to a magnetic separation method of staphylophylla brannica and a recovery method of the magnetic medium used therein. Background technique [0002] In order to realize the sustainable and stable development of the bioenergy industry, it is imminent to actively search for raw materials for the preparation of biodiesel and biomass oil. Microalgae is a kind of aquatic plant. Its unique chemical composition and structure make it an excellent source of raw materials for biodiesel and biomass oil. The use of microalgae to prepare biofuels has broad prospects for development and utilization. [0003] Brown botrytis (Botryococcus braunii, also translated as cluster algae, commonly known as "oil algae") is a freshwater unicellular green algae that grows in clusters. Because of its high hydrocarbon production capacity, it can be used as a non-polluting alternati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C01G49/08B82Y30/00B82Y40/00
Inventor 刘春朝郭晨王锋
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com