Botryococcus braunii magnetic separation method

A technology of botrytis brauneni and magnetic separation, applied in the direction of unicellular algae, ferrous oxides, nanotechnology, etc., can solve the problem of low yield and quality of botrytis braunenii cells, inability to regenerate and recycle the particles, and unproductive problems. Considering the coupling of oil extraction and separation medium recovery, the effects of easy scale-up, reduced production cost, and simple synthesis steps are achieved

Inactive Publication Date: 2012-01-18
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the magnetic nano-Fe used in it 3 o 4 The particles cannot be recycled and recycled, and it does not cons

Method used

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  • Botryococcus braunii magnetic separation method
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  • Botryococcus braunii magnetic separation method

Examples

Experimental program
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Example Embodiment

[0054] Example one

[0055] The high-efficiency recovery method of magnetic nanoparticles used in the magnetic separation of Botryococcus braunii of the present invention is used to perform Fe 3 O 4 The steps for the recovery of nanoparticles are as follows:

[0056] (1) The prepared Fe 3 O 4 The nanoparticles are dispersed in deionized water to form a stable suspension, formulated into a magnetic separation medium with a concentration of 1% (w / v), and then placed in a refrigerator for refrigeration.

[0057] (2) Put the culture broth of Botryococcus braunii into a stirred reactor, add a magnetic separation medium at a speed of 200 rpm, the amount of magnetic separation medium is 0.02% (w / v), and the stirring time is 1.5 minutes; After separation, the supernatant was removed, and the polymer of Botryococcus brevifolia cells and magnetic medium was collected, and the recovery rate of Botryococcus brevifolia reached 99.8%;

[0058] (3) At room temperature, add 5 mL / g of polymer ethylene...

Example Embodiment

[0062] Example two

[0063] The high-efficiency recovery method of magnetic nanoparticles used in the magnetic separation of Botryococcus braunii of the present invention is used to perform Fe 3 O 4 The steps for the recovery of nanoparticles are as follows:

[0064] (1) The prepared Fe 3 O 4 The nanoparticles are dispersed in deionized water to form a stable suspension, formulated into a magnetic separation medium with a concentration of 0.5% (w / v), and then placed in a refrigerator for storage.

[0065] (2) Put the Botryococcus culture solution obtained by the culture into a stirred reactor, add a magnetic separation medium at a speed of 100 rpm, the amount of the magnetic separation medium is 0.01% (w / v), and the stirring time is 2 minutes; magnetic separation After removing the supernatant liquid, collecting the polymer of the separated Botryococcus braunii cells and the magnetic medium, the recovery rate of Botryococcus braunii reaches 99.5%;

[0066] (3) At room temperature, add...

Example Embodiment

[0070] Example three

[0071] The high-efficiency recovery method of magnetic nanoparticles used in the magnetic separation of Botryococcus braunii of the present invention is used to perform Fe 3 O 4 The steps for the recovery of nanoparticles are as follows:

[0072] (1) The prepared Fe 3 O 4 The nanoparticles are dispersed in deionized water to form a stable suspension, formulated into a magnetic separation medium with a concentration of 2% (w / v), and then placed in a refrigerator for refrigeration.

[0073] (2) Put the Botryococcus culture solution obtained by the culture into a stirred reactor, add a magnetic separation medium at 300 rpm, the amount of the magnetic separation medium is 0.03% (w / v), and the stirring time is 1 minute; magnetic separation After removing the supernatant liquid, collecting the polymer of the separated Botryococcus braunii cells and the magnetic medium, the recovery rate of Botryococcus brauni reaches 99.3%;

[0074] (3) At room temperature, add 8 mL / g...

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Abstract

The invention dicloses a botryococcus braunii magnetic separation method for the regeneration cycle of a magnetic medium, which comprises the following steps: (1) dispersing Fe3O4 nano-particles to form a stable suspension; (2) adding the magnetic separation medium into a botryococcus braunii culture solution, stirring, performing magnetic separation to obtain a polymer of botryococcus braunii and the magnetic medium; (3) adding ethylene glycol dimethyl ether into the polymer for treatment for 2-10 min, adding n-hexane, stirring, and collecting residues; (4) washing the residues, adding hydrochloric acid for reaction, filtering after the reaction is finished, collecting the filtrate; (5) adjusting the pH of the filtrate to 0.8-1.5 by ammonia water, preparing Fe3O4 nano-particles with the filtrate as a raw material so as to realize the regeneration cycle of the magnetic medium. The method of the invention couples the oil extraction with the recovery of the separation medium, and realizes the recovery of the magnetic separation medium; the regeneration recovery of Fe3O4 nano-particles is realized by methods of hydrochloric acid dissolution and ammonia water recrystallization.

Description

technical field [0001] The invention belongs to the field of separation of microalgae, in particular, the invention relates to a magnetic separation method of staphylophylla brannica and a recovery method of the magnetic medium used therein. Background technique [0002] In order to realize the sustainable and stable development of the bioenergy industry, it is imminent to actively search for raw materials for the preparation of biodiesel and biomass oil. Microalgae is a kind of aquatic plant. Its unique chemical composition and structure make it an excellent source of raw materials for biodiesel and biomass oil. The use of microalgae to prepare biofuels has broad prospects for development and utilization. [0003] Brown botrytis (Botryococcus braunii, also translated as cluster algae, commonly known as "oil algae") is a freshwater unicellular green algae that grows in clusters. Because of its high hydrocarbon production capacity, it can be used as a non-polluting alternati...

Claims

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Application Information

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IPC IPC(8): C12N1/12C01G49/08B82Y30/00B82Y40/00
Inventor 刘春朝郭晨王锋
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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