Method for preparing corilagin through matsumura leafflower herb

Abstract

The invention discloses a method for preparing corilagin through a matsumura leafflower herb. The method comprises the steps of: crushing a matsumura leafflower herb medicinal material; performing Soxhlet extraction by adding 40-60 percent of ethanol; filtering an extracted solution; ultra-filtering by adding an ultra-filtration membrane system; concentrating a liquid permeating the ultra-filtration membrane system through a reverse osmosis membrane to obtain a concentrated liquid; regulating the pH value of the concentrated liquid to be pH 4-5; extracting through ethyl acetate; separating through a high-speed countercurrent chromatography after concentrating an extract liquid; collecting target components according to a chromatogram of an ultraviolet detector; and drying at a low temperature to obtain a product. By adopting the method for producing the corilagin, the energy consumption is low, the yield rate is high, and the product content is high.

Description

technical field

[0001] The invention belongs to the technical field of medicine, and in particular relates to a method for preparing corilagin from citrus citrus. Background technique

[0002] Corilagin (Corilagin), also known as myrobalan subtannin, vermiculite, etc., the chemical name is 1-O-galloyl-3,6-O-hexahydroxybiphenyldiformyl-β-D-glucose, Molecular formula: C 27 h 22 o 18 ; Molecular weight: 634.453, CAS accession number: 23094-69-1, molecular structure formula is as follows:

[0003]

[0004] Corilagin is a water-soluble inverse gallate tannin that can be extracted from many plants, and widely exists in a variety of plants, such as Citrus phyllophylla and Citrus citrus, Yak Ermiao, Geranium Plants of the genus Grass, Euphorbia, Amaranth, Pomegranate, Acer and Terminalia, etc. Studies in recent years have shown that Corilagin has antihypertensive activity, anti-HBV activity, and antithrombotic activity. Some Japanese patents disclose that Corilagin can incre...

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