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Herpesvirus II type polymerase chain reaction (PCR) fluorescent quantitative fast detection kit and method

A detection kit, fluorescence quantitative technology, applied in fluorescence/phosphorescence, microbial determination/inspection, biochemical equipment and methods, etc. specific effect

Active Publication Date: 2012-02-01
泰普生物科学(中国)有限公司
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Problems solved by technology

[0007] Although the conventional PCR method has the advantages of simplicity, speed, and sensitivity, it has problems such as inaccurate quantification and false positives caused by contamination caused by post-PCR post-processing. Therefore, it is necessary to develop an accurate, sensitive, fast, and stable clinical testing method.

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  • Herpesvirus II type polymerase chain reaction (PCR) fluorescent quantitative fast detection kit and method
  • Herpesvirus II type polymerase chain reaction (PCR) fluorescent quantitative fast detection kit and method
  • Herpesvirus II type polymerase chain reaction (PCR) fluorescent quantitative fast detection kit and method

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Embodiment Construction

[0022] The present invention uses fluorescent PCR technology to detect herpes simplex virus type II kit, that is, detects whether there is herpes simplex virus type II in clinically collected specimens, thereby guiding clinicians to use drugs for patients infected with herpes simplex virus type II, and helping to judge the prognosis .

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Abstract

The invention aims at providing a herpesvirus II type polymerase chain reaction (PCR) fluorescent quantitative fast detection kit, which comprises deoxyribose nucleic acid (DNA) extraction liquid, PCR reaction liquid, DNA polymerase, a positive quality control article, a weak positive quality control article, a negative quality control article and a quantitative reference article, wherein the PCRreaction liquid comprises a primer and a fluorescent probe. The kit has the beneficial effects that the kit uses the positive comparison and the negative comparison, the accuracy of the kit is improved, and in addition, nucleic acid DNA with the content lower than 5*10<2> gene copy / mL can also be detected. The nucleic acid amplification method has the advantages of high sensitivity, high specificity and good repetitiveness. The invention also uses an arabidopsis inside reference system, and homologous genes do not exist in pure herpesvirus II type genomes and human genomes, so the kit can be used as the inside reference for detecting whether PCR inhibiting substances exist in each PCR reaction, and the accuracy of PCR results is ensured.

Description

technical field [0001] The invention relates to a disease pathogen gene detection technology, in particular to a herpes virus type II PCR fluorescence quantitative rapid detection kit and method. Background technique [0002] Herpes simplex virus (herpess simplex virus) belongs to the herpesviridae alpha virus subfamily, and the size of the virus plasmid is about 180 nanometers. Human herpes simplex virus is divided into two types, namely herpes simplex virus type I (HSV-I) and herpes simplex virus type II (HSV-II). HSV infection is due to person-to-person contact. It can cause herpetic keratitis, herpetic dermatitis, genital herpes, etc. The incidence of fetal HSV infection is 0.4% to 1.0%. It can cause fetal intrauterine infection, induce fetal abortion, premature birth, stillbirth and deformity, and can also infect newborns. Son. Herpes simplex virus type II can cause herpetic keratitis, herpetic dermatitis, genital herpes, etc., and type I mainly causes infections of ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
Inventor 何东华姚雪
Owner 泰普生物科学(中国)有限公司
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