Herpesvirus II type polymerase chain reaction (PCR) fluorescent quantitative fast detection kit and method

Abstract

The invention aims at providing a herpesvirus II type polymerase chain reaction (PCR) fluorescent quantitative fast detection kit, which comprises deoxyribose nucleic acid (DNA) extraction liquid, PCR reaction liquid, DNA polymerase, a positive quality control article, a weak positive quality control article, a negative quality control article and a quantitative reference article, wherein the PCRreaction liquid comprises a primer and a fluorescent probe. The kit has the beneficial effects that the kit uses the positive comparison and the negative comparison, the accuracy of the kit is improved, and in addition, nucleic acid DNA with the content lower than 5*10<2> gene copy/mL can also be detected. The nucleic acid amplification method has the advantages of high sensitivity, high specificity and good repetitiveness. The invention also uses an arabidopsis inside reference system, and homologous genes do not exist in pure herpesvirus II type genomes and human genomes, so the kit can be used as the inside reference for detecting whether PCR inhibiting substances exist in each PCR reaction, and the accuracy of PCR results is ensured.

Description

technical field [0001] The invention relates to a disease pathogen gene detection technology, in particular to a herpes virus type II PCR fluorescence quantitative rapid detection kit and method. Background technique [0002] Herpes simplex virus (herpess simplex virus) belongs to the herpesviridae alpha virus subfamily, and the size of the virus plasmid is about 180 nanometers. Human herpes simplex virus is divided into two types, namely herpes simplex virus type I (HSV-I) and herpes simplex virus type II (HSV-II). HSV infection is due to person-to-person contact. It can cause herpetic keratitis, herpetic dermatitis, genital herpes, etc. The incidence of fetal HSV infection is 0.4% to 1.0%. It can cause fetal intrauterine infection, induce fetal abortion, premature birth, stillbirth and deformity, and can also infect newborns. Son. Herpes simplex virus type II can cause herpetic keratitis, herpetic dermatitis, genital herpes, etc., and type I mainly causes infections of ...

AI Technical Summary

Problems solved by technology

[0007] Although the conventional PCR method has the advantages of simplicity, speed, and sensitivity, it has problems such as inaccurate quantification and false positives caused by contamination caused by post-PCR post-processing. Therefore, it is necessary to develop an accurate, sensitive, fast, and stable clinical testing method.

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