Method for detecting steroid hormones in urine
A steroid hormone and urine technology, applied in the field of bioanalytical chemistry, can solve the problems of expensive instruments and consumables, difficult general application, complicated operation, etc., and achieve the effects of lowering detection limit, good enrichment effect and simple operation.
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Embodiment 1
[0018] The invention is used to detect the content of estradiol hormone in human urine.
[0019] Mix 100 μL of carbon tetrachloride and 0.5 mL of acetone, quickly inject the mixture with a syringe into 5.0 mL of urine sample adjusted to pH 7.0 with disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and sonicate at 35 kHz to Form a uniform emulsified system, centrifuge in a centrifuge at 10,000r / min for 2min, remove the upper layer solution with a pipette, and dry the bottom organic phase with a nitrogen blower, then dissolve the obtained solid in acetonitrile and dilute to 1mL. The resulting sample solution and 0.1 μg / mL estradiol standard solution were spotted on a thin-layer plate with a length of 8 cm, and developed in chloroform-methanol solution (7:3, v / v) to the distance from the front of the solution to the thin-layer plate. The end 2cm, after drying, evenly spray 10% (w / v) anisaldehyde ethanol chromogenic agent, baked under infrared light without ...
Embodiment 2
[0021] The invention is used to detect the content of progesterone hormone in human urine.
[0022] Mix 100 μL of dichloromethane and 0.5 mL of acetonitrile, quickly inject the mixture with a syringe into 5.0 mL of urine sample adjusted to pH 7.0 with disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and sonicate at 40 kHz to form For a uniform emulsified system, centrifuge at 5000r / min for 4min in a centrifuge, remove the upper layer solution with a straw, and dry the bottom organic phase with a nitrogen blower, then dissolve the obtained solid in acetonitrile and dilute to 1mL. Spot the obtained sample solution and 0.1 μg / mL progesterone standard solution on a thin-layer plate with a length of 9 cm, and spread it in chloroform-methanol solution (7:3, v / v) until the front of the solution is 2 cm from the end of the thin-layer plate After drying, evenly spray 12% (w / v) anisaldehyde ethanol color developer, and bake under infrared light to show blue color...
Embodiment 3
[0024] The invention is used to detect the content of methyltestosterone hormone in human urine.
[0025] Take 100 μL of carbon tetrachloride and 0.5mL of 0.05% TritonX-100 non-ionic surfactant and mix well, quickly inject the mixed solution into 5.0mL of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution to adjust the pH value to 7.0 with a syringe In the urine sample, sonicate at 45kHz until a uniform emulsion system is formed, centrifuge at 4000r / min in a centrifuge for 5min, remove the upper layer solution with a straw, dry the bottom organic phase with a nitrogen blower, and use Dissolve in acetonitrile to 1 mL. Spot the obtained sample solution and 0.1 μg / mL methyltestosterone standard solution on a thin-layer plate with a length of 10 cm, and spread it in chloroform-methanol solution (7:3, v / v) until the front of the solution is 2 cm from the end of the thin-layer plate , evenly spray 15% (w / v) anisaldehyde ethanol chromogenic agent after drying, b...
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