Application of Pouzolzia extract
A technology of water extract and kudzu fumigatus, which is applied in the field of medicine, can solve the problem that the medicinal value of kudzu vulgare has not been fully developed and utilized, the pharmacological and medicinal components have not been reported, and the preparation and application of the extract of pus-pulling and anti-inflammatory has not been reported. application and other issues, to achieve good application prospects and industrial practical value, simple preparation methods, and rich raw material resources.
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Embodiment 1
[0056] The preparation of embodiment 1 extract
[0057] Take 100g of Mishuige whole grass, remove impurities, cut into pieces, add 1500mL of distilled water, heat and reflux to extract 3 times, each time 2h, combine the 3 extracts, concentrate into extract, add water to volume 100mL, and make a concentration of 1g of crude drug / mL of liquid medicine; 50mL of the liquid medicine was taken as the total extract (P) of Pueraria mist water, and the other 50mL was dispersed with a small amount of water, and extracted with chloroform, ethyl acetate, and n-butanol in sequence until the color of each extract was light, and the combined The extract (chloroform extract PC, ethyl acetate extract PE, n-butanol extract PB) and the last water part (PW) were concentrated under reduced pressure to liquid extract.
Embodiment 2
[0058] The preparation of embodiment 2 extract
[0059] Take 10kg of Pueraria japonica, remove impurities, cut into pieces, add 150L of distilled water, heat and reflux 3 times, 2 hours each time, combine the extracts 3 times, concentrate into an extract, disperse with a small amount of water, and wash with chloroform, ethyl acetate, Extract with n-butanol until the color of each extract is light, and concentrate each extract under reduced pressure to liquid extract.
[0060] Take the extract from the chloroform extraction part and carry out silica gel (100-200 mesh) column chromatography separation, chloroform:methanol (300:1-1:1, volume ratio) gradient elution, and combine similar fractions after detection by thin-layer chromatography. Obtained 7 components Fra.A-G. Take fraction Fra.B and proceed to silica gel (300-400 mesh) column chromatography (mass ratio of sample to silica gel is 1:50), and gradient elution with chloroform-methanol (200:1-20:1, volume ratio), 34 frac...
Embodiment 4
[0074] The experiment of embodiment 4 extracts to mouse subcutaneous abscess
[0075](1) Experimental materials: erythromycin ointment (Guangzhou He Jigong Pharmaceutical Factory, batch number: S1019); Pulling pus ointment (hospital preparation of Foshan Hospital of Traditional Chinese Medicine, batch number: 100921); 10% formaldehyde fixative (Shandong Tuobo Plastic Co., Ltd., Lot No. 100226); Mishui Kudzu Extract, homemade.
[0076] (2) Experimental animals: SPF grade NIH male mice (body weight 18-22 g), purchased from the Medical Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine, certificate number: SCXK (Guangdong) 2008-0020.
[0077] (3) Experimental method: select 10% (mass percentage concentration) starch aqueous solution as the external liniment matrix, add each liquid extract in Example 1 into the matrix respectively, so that the total volume is 50mL, and prepare Mishui Geshui External liniment for each extraction part of the extract....
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