Vibrio parahaemolyticus chromogenic medium and rapid detection card for the same

Abstract

The invention discloses a Vibrio parahaemolyticus chromogenic medium and a rapid detection card for the same. The chromogenic medium contains peptone, yeast powder, sodium chloride, cane sugar, beta-glucoside, sodium thiosulfate and biological buffer; and the rapid detection card is a steady microorganism culture device comprising a base plate, a water-absorbing gel layer, a medium layer and a cover film, wherein the chromogenic medium is absorbed on the medium layer. In the Vibrio parahaemolyticus chromogenic medium, the beta-glucoside is used as the color development reagent, and the biological buffer is added, so that detection accuracy is improved greatly, qualitative and quantitative analysis can be carried out for the Vibrio parahaemolyticus chromogenic medium, and positive result color development is obvious and convenient to observe. The rapid detection card provided by the invention has the advantages of short detection cycle, simple and convenient operation, high sensitivity, low cost and the like, and is suitable for processing high-flux samples.

Description

technical field [0001] The invention belongs to the field of food safety microbiological testing, and relates to a color-developing culture medium for Vibrio parahaemolyticus, and also relates to a quick detection card made by using the color-developing culture medium. Background technique [0002] Food is the material basis for human survival. Ensuring the safety and sanitation of food and preventing toxic and harmful substances from causing harm to the human body through food are major issues related to human health and social development. In recent years, vicious incidents of food safety at home and abroad have continued to occur, among which food-borne pathogens are an important cause of food safety accidents, and about 2.2 million people in the world die each year due to food-borne pathogens. Therefore, food-borne Disease control has become a common concern at home and abroad. [0003] Vibrio parahaemolyticus (VP ​​for short) is a common type of foodborne pathogenic ba...

AI Technical Summary

Problems solved by technology

[0005] At present, the traditional detection method of Vibrio parahaemolyticus is as follows: the sample is separated and cultivated, microscopic observation, biochemical identification, halophilic test, etc., the operation is cumbersome, the procedure is complicated, the reagents used are various, time-consuming and labor-intensive, the detection cycle is long, and it takes 4 ~7 days, and the detection efficiency is low and the sensitivity is low; in recent years, a large number of reports have confirmed that the PCR-based method has been successfully used to detect Vibrio parahaemolyticus, although it has the characteristics of good specificity and high sensitivity, but it is usually Requires expensive and sophisticated instruments and equipment, and has relatively high technical requirements for the experimental environment and operators

Immunological detection technology is fast, simple, and low-cost, but requires high-quality and high-stability monoclonal antibodies, otherwise it will lead to insufficient accuracy. Currently, it can only be used as an auxiliary detection method

In addition, there are some detection methods, such as immunochromatography and DNA hybridization. Although the detection sensitivity is high, the equipment required is expensive, the operation process is relatively complicated, and it is difficult to popularize and apply.

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