Rhizopus oryzae TY GF1 and application of its whole cell catalysis of chrysalisoil in biodiesel preparation
A technology of biodiesel and rhizopus oryzae, applied in the field of microorganisms, can solve the problems of difficult separation of by-product glycerin, difficulty in removing catalyst, pollution discharge and other problems
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Embodiment 1
[0015] Rhizopus oryzae (Rhizopus oryzae) TY GF1 cells that grew well on the slant were transferred to the enzyme-producing medium. Inoculate the bacteria in a culture medium with a temperature of 35°C, and culture it in shake flasks for 2 days. The pH of the culture medium is 5.0. In each liter of culture medium: 5 g of sucrose, 2 g of peptone, 5 g of canola oil, 1.2 g of sodium nitrate, and sulfuric acid heptahydrate Magnesium 0.5g, potassium dihydrogen phosphate 1.2g, Tween-80 1.5%; the cultured fermentation mixture was filtered under reduced pressure, and the obtained mycelium was freeze-dried for 12 hours before use. Prepare transesterification reaction system, in each 100mL Erlenmeyer flask: silkworm chrysalis oil 6g, methanol 0.35g, whole cell (mycelium) 0.5g, 0.2M sodium acetate buffer (pH5.0) 0.2mL, add once every 24h Methanol, a total of three times, the rotation speed is 150rpm, the reaction time is 72h, and the fermentation broth is analyzed by gas chromatography. ...
Embodiment 2
[0017] This example illustrates the characteristics of Rhizopus oryzae (Rhizopus oryzae) TY GF1 in catalyzing the production of biodiesel from silkworm pupa oil based on the addition of 0.8 g of whole cells.
[0018] Transesterification reaction system, in each 100mL Erlenmeyer flask: silkworm chrysalis oil 6g, methanol 0.35g, 0.2M sodium acetate buffer (pH5.0) 0.2mL, whole cells (mycelium) 0.8g, add methanol once every 24h , a total of three times, the rotation speed was 150rpm, and the reaction time was 72h. The fermentation broth was analyzed by gas chromatography, and the conversion rate of biodiesel was 54.7%.
Embodiment 3
[0020] The method of this example is the same as that of Example 2, but the amount of whole cells added is changed to 0.5 g, and the conversion rate of biodiesel is detected.
[0021] Transesterification reaction system, in each 100mL Erlenmeyer flask: silkworm chrysalis oil 6g, methanol 0.35g, 0.2M sodium acetate buffer (pH5.0) 0.2mL, whole cells (mycelium) 0.8g, add methanol once every 24h , a total of three times, with a rotation speed of 150 rpm and a reaction time of 72 hours. The fermentation broth was analyzed by gas chromatography, and the conversion rate of biodiesel was 35.3%.
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