Rhizopus oryzae TY GF1 and application of its whole cell catalysis of chrysalisoil in biodiesel preparation

A technology of biodiesel and rhizopus oryzae, applied in the field of microorganisms, can solve the problems of difficult separation of by-product glycerin, difficulty in removing catalyst, pollution discharge and other problems

Inactive Publication Date: 2014-04-16
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, it overcomes the problems of chemical method (acid method or alkali method) of high energy consumption, difficulty in separation of by-product glycerin, complicated product post-treatment, difficulty in removing catalyst, and pollution discharge.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Rhizopus oryzae (Rhizopus oryzae) TY GF1 cells that grew well on the slant were transferred to the enzyme-producing medium. Inoculate the bacteria in a culture medium with a temperature of 35°C, and culture it in shake flasks for 2 days. The pH of the culture medium is 5.0. In each liter of culture medium: 5 g of sucrose, 2 g of peptone, 5 g of canola oil, 1.2 g of sodium nitrate, and sulfuric acid heptahydrate Magnesium 0.5g, potassium dihydrogen phosphate 1.2g, Tween-80 1.5%; the cultured fermentation mixture was filtered under reduced pressure, and the obtained mycelium was freeze-dried for 12 hours before use. Prepare transesterification reaction system, in each 100mL Erlenmeyer flask: silkworm chrysalis oil 6g, methanol 0.35g, whole cell (mycelium) 0.5g, 0.2M sodium acetate buffer (pH5.0) 0.2mL, add once every 24h Methanol, a total of three times, the rotation speed is 150rpm, the reaction time is 72h, and the fermentation broth is analyzed by gas chromatography. ...

Embodiment 2

[0017] This example illustrates the characteristics of Rhizopus oryzae (Rhizopus oryzae) TY GF1 in catalyzing the production of biodiesel from silkworm pupa oil based on the addition of 0.8 g of whole cells.

[0018] Transesterification reaction system, in each 100mL Erlenmeyer flask: silkworm chrysalis oil 6g, methanol 0.35g, 0.2M sodium acetate buffer (pH5.0) 0.2mL, whole cells (mycelium) 0.8g, add methanol once every 24h , a total of three times, the rotation speed was 150rpm, and the reaction time was 72h. The fermentation broth was analyzed by gas chromatography, and the conversion rate of biodiesel was 54.7%.

Embodiment 3

[0020] The method of this example is the same as that of Example 2, but the amount of whole cells added is changed to 0.5 g, and the conversion rate of biodiesel is detected.

[0021] Transesterification reaction system, in each 100mL Erlenmeyer flask: silkworm chrysalis oil 6g, methanol 0.35g, 0.2M sodium acetate buffer (pH5.0) 0.2mL, whole cells (mycelium) 0.8g, add methanol once every 24h , a total of three times, with a rotation speed of 150 rpm and a reaction time of 72 hours. The fermentation broth was analyzed by gas chromatography, and the conversion rate of biodiesel was 35.3%.

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Abstract

Rhizopus oryzae TY GF1 and an application of its whole cell catalysis chrysalisoil in biodiesel preparation. The collection date of a Rhizopus oryzae TY GF1 strain is on December 20th, 2010. The collection registry number is CGMCC No. 4490. The intracellular lipase production condition of the Rhizopus oryzae TY GF1 strain and its whole cell catalysis chrysalisoil in the preparation of biodiesel.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to conditions for producing intracellular lipase by a strain of Rhizopus oryzae TYGF1 and the application of whole-cell catalysis to prepare biodiesel. Background technique [0002] Prior art: Biodiesel refers to fatty acid methyl ester produced by transesterification of vegetable oil and methanol, and is a clean biofuel. Compared with conventional diesel oil, fatty acid methyl ester has some incomparable characteristics: ① high flash point, good safety performance; ② low viscosity, cetane number greater than 49 (the cetane number of petroleum diesel is only 45), anti-knock Good performance and full combustion; ③Excellent environmental protection performance. On the one hand, because it does not contain sulfur, it will not cause the formation of acid rain; on the other hand, the CO produced by its combustion 2 CO absorbed by its plant growth 2 It is basically the sam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/845
CPCY02E50/13Y02E50/10
Inventor 吴福安王俊方水琴邱宁张磊霞郭锡杰
Owner JIANGSU UNIV OF SCI & TECH
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