Method for producing glutathione by fermenting sweet sorghum stalk juice

A glutathione and sweet sorghum technology, which is applied in the field of glutathione production by fermentation of sweet sorghum stalk juice, can solve the problems of compressed enterprise profit margins and high production costs, and achieve huge economic and social benefits and production costs Low, food-saving effect

Inactive Publication Date: 2012-03-14
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glutathione is a high value-added product. At present, the medium commonly used by yeast to produce glutathione is a medium synthesized by using glucose as a carbon source and adding nitrogen sources, inorganic salts, growth factors and other raw materials, resulting in The high production cost reduces the profit margin of the enterprise

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Strain: Saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC1955 (preserved by China Industrial Microorganism Culture Collection Management Center). The sweet sorghum stalks are taken from the 100-acre saline-alkaline sweet sorghum production demonstration field of Guangbei Farm in Dongying, and the juice yield of the stalks is about 60wt%. The press used to extract juice from sweet sorghum stalks is a sugarcane press produced by Guangxi Debao County Machinery Factory, with a production capacity of 1T / h.

[0033] A method for producing glutathione by fermenting sweet sorghum stalk juice, the steps are as follows:

[0034] (1) Inoculate yeast cells with glutathione synthetase and glycolytic activity on the slant medium for activation, culture at 28°C for 48 hours, then take yeast cells from the slant medium and insert them into 10mL liquid seed medium , cultured at 28°C for 24 hours, carried out a primary expansion culture, then inserted a 10wt% inoculum into the li...

Embodiment 2

[0046] The method for producing glutathione by fermentation of sweet sorghum stalk juice as described in Example 1, the difference is that:

[0047] Dilute the sugar content of sweet sorghum stalk juice to 4wt%, take 200mL of sorghum stalk juice fermentation medium and put it into a 1000mL shake flask, add yeast strains that have been cultivated at 30°C for 16h, and the inoculation amount of yeast is 10wt %, the rotation speed of the shaker flask is 200r / min, the fermentation temperature is 30°C, and the fermentation time is 36h.

[0048] After testing, the content of glutathione was 474mg / L.

Embodiment 3

[0050] The method for producing glutathione by fermentation of sweet sorghum stalk juice as described in Example 1, the difference is that:

[0051] Adopt a 10L fully automatic fermenter, the filling volume of the culture medium described in Example 1 is 5L, the yeast inoculation amount is 10wt%, the fermentation temperature is 30~32°C, the fermentation time is 36h, and the dissolved oxygen saturation is controlled at 30%. . Other conditions are with embodiment 1. Under these conditions, the content of glutathione is 685mg / L.

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PUM

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Abstract

The invention relates to a method for producing glutathione by fermenting sweet sorghum stalk juice. The method comprises the following steps of: (1) preparing active yeast cells; (2) preparing a fermentation culture medium; (3) inoculating the active yeast cells prepared in step (1) in the fermentation culture medium prepared in step (2) for fermentation; and (4) extracting the glutathione in the yeast cells prepared in step (3). Based on the study, stalk juice of sweet sorghum in saline-alkali soil can be directly taken as the fermentation culture medium, without adding other nutrient substances, so that the glutathione produced from the sweet sorghum stalk juice needs lower production cost than that from glucose, and has high quality; and moreover, grains are saved, and national industrial policies are met.

Description

technical field [0001] The invention relates to a method for producing glutathione by yeast, in particular to a method for producing glutathione by fermenting sweet sorghum stalk juice, and belongs to the technical field of biotechnology. Background technique [0002] Glutathione (GSH) is a substance with important physiological activities. It is a tripeptide compound formed by condensation of glutamic acid, cysteine ​​and glycine through peptide bonds. It is a widely used active polypeptide. , its chemical name is γ-L-glutamine-L-cysteine-glycine, and its molecular formula is C 10 h 18 o 6 N 3 S. Glutathione has two forms, oxidized and reduced. Reduced glutathione has physiological activity, while oxidized glutathione in organisms needs to be reduced to exert its physiological functions. [0003] Glutathione widely exists in animals, plants and microorganisms. The physiological functions of glutathione (GSH) in organisms are various, such as the transport of amino aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/02C12R1/865C12R1/84C12R1/72
Inventor 董永胜
Owner QILU UNIV OF TECH
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