Separation and purification process for L-tryptophane

A technology for separation and purification of tryptophan, applied in the field of separation and purification of L-tryptophan

Inactive Publication Date: 2012-03-21
北京明新高科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a novel process for separating and purifying L-tryptophan from fermentation broth, so as to solve the problems in the prior art

Method used

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  • Separation and purification process for L-tryptophane

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Comparison scheme
Effect test

Embodiment 1

[0024] After diluting 5 L of L-tryptophan fermentation liquid with a concentration of 28.4 g / L by 1 time, use a 0.2 μm microfiltration membrane to filter and sterilize at 60° C. to obtain 9.5 L of sterilized liquid.

[0025] The sterilizing solution is ultrafiltered through a membrane with a molecular weight cut-off of 1000. The ultrafiltration temperature is 60°C to remove protein, macromolecular impurities and pigments. The pH of the permeate is adjusted to 2.5 with hydrochloric acid, and the ionic strength is adjusted to 0.15M by adding KCl to obtain 9.3 L of extracted aqueous phase, ready for reverse micelles extraction.

[0026] Adopt centrifugal extractor to carry out three-stage counter-current extraction to the filtrate that adjusts ion concentration and pH value, extraction condition is: organic phase: water phase=1: 2, organic phase is AOT / isooctane (AOT concentration is 60mmol / L) , three-stage countercurrent extraction was carried out at a temperature of 20° C. and ...

Embodiment 2

[0030] After diluting 4 L of L-tryptophan fermentation broth with a concentration of 32.5 g / L by 1 time, use a 0.15 μm microfiltration membrane to filter and sterilize at 80° C. to obtain 7.8 L of sterilized solution.

[0031] The sterilizing solution is ultrafiltered through a membrane with a molecular weight cut-off of 1000. The ultrafiltration temperature is 60°C to remove protein, macromolecular impurities and pigments. The pH of the permeate is adjusted to 2.5 with hydrochloric acid, and the ionic strength is adjusted to 0.15M by adding KCl to obtain 7.5 L of the extracted aqueous phase, ready for reverse micelles extraction.

[0032] Adopt centrifugal extractor to carry out three-stage countercurrent extraction to the filtrate that has adjusted ion concentration and pH value, and extraction condition is: organic phase: aqueous phase=1: 5, and organic phase is AOT / isooctane (AOT concentration is 80mmol / L) , the organic phase and the aqueous phase were mixed for three-sta...

Embodiment 3

[0036] After diluting 2L of L-tryptophan fermentation broth with a concentration of 26g / L by 1 time, use a 0.05μm microfiltration membrane to filter and sterilize at 40°C to obtain 3.8L of filtrate.

[0037] The filtrate was ultrafiltered through a membrane with a molecular weight cut-off of 1000. The ultrafiltration temperature was 40°C to remove protein, macromolecular impurities and pigments. The pH of the permeate was adjusted to 2.0 with hydrochloric acid, and the ionic strength was adjusted to 0.10M by adding KCl to obtain 3.5L The extracted aqueous phase is ready for reverse micellar extraction.

[0038] Adopt centrifugal extractor to carry out three-stage countercurrent extraction to the filtrate that adjusts pH value and ion concentration, extraction condition is: organic phase: water phase=1: 1, organic phase is AOT / isooctane (AOT concentration is 40mmol / L) , the organic phase and water were subjected to three-stage countercurrent extraction at an equal volume ratio,...

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Abstract

The invention discloses a method for separating and purifying L-tryptophane from fermentation liquor. Through microfiltration, ultrafiltration and adjustment to pH values and ionic strength, three-stage counter-current extraction and three-stage counter-current back extraction are performed on dilute L tryptophane fermentation liquor by adopting reverse micelle extraction technology, and L-tryptophane with the content higher than 98.0 percent is obtained from aqueous phase enriched with L-tryptophane through secondary nanofiltration, crystallization and centrifugal drying. The method disclosed by the invention adopts the membrane separation technology, the reverse micelle extraction technology and the like, has the characteristics of high extraction yield, simple extraction process, speediness, effectiveness, recyclability in using extracting agent and the like, can save operating cost, and is convenient for industrial magnification and continuous operation.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a method for separating and purifying L-tryptophan from fermentation broth. Background technique [0002] Tryptophan is one of the eight essential amino acids in the human body. It is a neutral aromatic amino acid containing an indole group. Its molecular formula is C 11 h 12 N 2 o 2 , molecular weight 204.23, white or slightly yellow leaf-like crystal or powder, solubility in water 11.4g / L (25°C), soluble in dilute acid or dilute alkali, stable in alkali, decomposed in strong acid, slightly soluble in ethanol, Insoluble in chloroform and ether, it plays an important role in the growth and metabolism of humans and animals. It is called the second essential amino acid and is widely used in medicine, food and feed, etc. According to the estimates of the Food and Agriculture Organization of the United Nations, the annual demand for L-tryptophan in the world market is about 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D209/20
Inventor 杨旭锦
Owner 北京明新高科技发展有限公司
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