Arthrobacter chlorophenolicus L4 and application thereof
A technology of Arthrobacter chlorophene and microbial strains, applied in the field of microorganisms, can solve the problems of not necessarily adapting to the red soil soil environment, achieve good growth-promoting effects, promote growth and development, and improve utilization rates
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Embodiment 1
[0029] Aerobic test
[0030] Pour the sterilized LB medium into 3 sterilized test tubes, at about 2 / 3, on the aseptic operating table, use an inoculation needle to pick up the bacteria cultured on the slant, puncture and inoculate into the above-mentioned culture base (must pierce to the bottom of the tube). Cultivate at 30°C, and observe the results at 3 days to 7 days respectively. Those that grow on the surface of the agar column are aerobic bacteria, and those that grow along the puncture line are anaerobic or facultative anaerobic bacteria. The test results showed that the colony grew along the surface of the agar column, and there was no colony growth in the puncture line, which was strictly aerobic.
[0031] Determination of catalase
[0032] Put 1 drop of 3% H on a clean slide 2 o 2 , take 1 ring of LB slant culture for 18~24 h, in H 2 o 2 Smear in the middle, if bubbles are produced, it is positive, otherwise it is negative. The test result was catalase posit...
Embodiment 2
[0057] First prepare the following three media.
[0058] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH 7.0-7.2, sterilized at 121°C for 20min.
[0059] LB liquid medium: without adding agar, other conditions are the same as above.
[0060] Inorganic phosphorus bacteria medium (PKO medium): 5g tricalcium phosphate, 10g glucose, 0.5g ammonium sulfate, 0.3g sodium chloride, 0.3g magnesium sulfate heptahydrate, 0.3g potassium chloride, 0.03g manganese sulfate, heptahydrate Ferrous sulfate 0.03g, pH 7.0 agar 20g, distilled water 1000ml, pH 7.0~7.2. Sterilize at 121°C for 20min.
[0061] Inorganic salt medium: ammonium sulfate 2.0g; sodium dihydrogen phosphate 0.5g; dipotassium hydrogen phosphate 0.5g; magnesium sulfate heptahydrate 0.2 g; calcium chloride dihydrate 0.1g, distilled water 1000mL, pH 7.0, 20min.
[0062] The red loam soil collected from Yingtan, Jiangxi was placed in a pot, and then the peanut seeds were sown in...
Embodiment 3
[0071] In order to further verify the ability and optimal conditions of Arthrobacter chlorophenes L4 to produce indoleacetic acid obtained in Example 1, the influence of different pH, liquid loading, different carbon sources, and different nitrogen sources on the production of indoleacetic acid will be explored below.
[0072] Adjust the LB medium containing L-tryptophan (100mg / L) to different pH (4, 5, 6, 7, 8, 9, 10), take 50mL and put it in a 250mL Erlenmeyer flask, press 1 %(v / v) inoculum amount After inoculating L4 in the logarithmic growth phase, place at 30°C, 180r·min -1 Cultivate on a shaking table for 24h, measure the amount of producing IAA by the method of quantitative determination, the results are as follows: figure 2 As shown, it shows that IAA is not produced when the pH is 4 and 10. In a strong acid and strong alkali environment, the bacteria cannot grow and metabolize. The strain produces more IAA in a slightly acidic environment than in an alkaline environm...
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