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Preparation method for nanometer graphene carrier used for magnetic resonance imaging (MRI) contrast agent

A magnetic resonance imaging and nanocarrier technology, applied in the field of functional graphene preparation, can solve the problems of high cytotoxicity and short retention time of contrast agents, and achieve the effects of reducing the toxicity of contrast agents, having good application prospects and prolonging the retention time.

Inactive Publication Date: 2012-04-04
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the above-mentioned defects in the prior art and provide a magnetic resonance imaging contrast agent that can be used as a variety of different types of contrast agent carriers and solve the problems of short retention time and high cytotoxicity of contrast agents in the body. Preparation method of graphene nanocarrier

Method used

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  • Preparation method for nanometer graphene carrier used for magnetic resonance imaging (MRI) contrast agent
  • Preparation method for nanometer graphene carrier used for magnetic resonance imaging (MRI) contrast agent
  • Preparation method for nanometer graphene carrier used for magnetic resonance imaging (MRI) contrast agent

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Embodiment 1

[0026] A method for preparing a graphene nanocarrier for a magnetic resonance imaging contrast agent, the method specifically comprising the following steps:

[0027] Preparation of Graphene Oxide

[0028] (1) Place 1g of flake graphite, 0.75g of sodium nitrate and 75mL of 98% concentrated sulfuric acid in an ice-water bath and stir, then slowly add 4.5g of potassium permanganate solid within one hour to ensure that the temperature is below 20°C. Stirring at room temperature for 5 days;

[0029] (2) Under ice-water bath conditions, slowly add 2.25 g of solid potassium permanganate to the mixture, keep the ice-water bath conditions to continue the reaction for 2 h, and the mixture continues to stir at room temperature for 5 days;

[0030] (3) repeat step (2) to obtain fully oxidized graphene twice;

[0031] (4) Add 140ml of dilute sulfuric acid with a mass fraction of 5% to the fully oxidized graphene obtained in step (3), stir at 98°C for 2h, then drop the temperature to 60°...

Embodiment 2

[0046] A preparation method of graphene nano-carriers for magnetic resonance imaging contrast agents, the method uses nano-graphene oxide and hydrophilic polymers as raw materials, grafts hydrophilic polymers on the surface of nano-graphene oxides, and then The agent is loaded into it.

[0047] The method specifically includes the following steps:

[0048] (1) Add polylysine to the graphene oxide solution whose concentration is 0.8mg / ml, the weight ratio of graphene oxide and polylysine is 1: 8, the mixture is ultrasonically dispersed for 4min, EDC and NHS catalyzed condensation, Adjust the pH to 5.6, continue to stir at room temperature for 20h, then transfer the obtained product to a 10,000 molecular weight dialysis bag, and dialyze in deionized water to obtain a pure graphene nanocarrier;

[0049](2) the graphene nanocarrier obtained by manganese-containing small molecule contrast agent chelate compound meso-porphyrin manganese (Mn-meso) and step (1) is dissolved in water ...

Embodiment 3

[0051] A preparation method of graphene nano-carriers for magnetic resonance imaging contrast agents, the method uses nano-graphene oxide and hydrophilic polymers as raw materials, grafts hydrophilic polymers on the surface of nano-graphene oxides, and then The agent is loaded into it.

[0052] The method specifically includes the following steps:

[0053] (1) Add chitosan to the graphene oxide solution with a concentration of 1.2mg / ml, the weight ratio of graphene oxide and chitosan is 1: 12, the mixture is ultrasonically dispersed for 6min, EDC and NHS are catalyzed and condensed, and the pH is adjusted 5.6, continue to stir at room temperature for 30h, then the product obtained is transferred to a dialysis bag with a molecular weight of 10,000, and dialyzed in deionized water to obtain pure graphene nanocarriers;

[0054] (2) the graphene nanocarrier obtained by the small molecule contrast agent chelate compound diethylenetriaminepentaacetate gadolinium diethylenetriaminep...

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Abstract

The invention relates to a preparation method for a nanometer graphene carrier used for an MRI contrast agent. According to the method, nanometer graphene oxide and hydrophilic high polymers are used as raw materials; the hydrophilic high polymers are grafted onto the surface of nanometer graphene oxide, and a contrast agent is loaded wherein. Compared to the prior art, the invention has the following advantages: by utilizing good hydrophilicity and biocompatibility of some hydrophilic high polymers and the characteristic that the system of nanometer graphene oxide and hydrophilic high polymers can enter into biological cells at a nanometer level, the contrast agent is enabled to have long in-vivo retention time, good histocompatibility and low cytotoxicity; the system shows a good application prospect in the aspect of MRI radiography.

Description

technical field [0001] The invention relates to a method for preparing functional graphene, in particular to a method for preparing a graphene nanocarrier for magnetic resonance imaging contrast agents. Background technique [0002] Magnetic Resonance Imaging Magnetic Resonance Imaging (MRI) is one of the important means of tumor diagnosis, especially the application of various contrast agents. However, most of the contrast agents used in clinical MRI examinations are small-molecule contrast agents containing gadolinium, which have many shortcomings such as low specificity and short retention time in the body. The construction of a new generation of MRI contrast agents with tissue specificity has become an important issue in the material and medical fields. one of the research hotspots. [0003] Nano-graphene oxide is a two-dimensional carbon nanomaterial with good water dispersibility, and its preparation cost is low. After being chemically modified with a hydrophilic pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K49/14A61K49/18C01B31/04
Inventor 李永勇沈爱军赵志磊王培军董海青
Owner TONGJI UNIV
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