Quantitative measurement method for theanine

A technology for quantitative determination of theanine, which is applied in the field of biochemistry, can solve the problems of uneven color spots, waste of medicines, and inaccurate determination, and achieve the effect of simple determination steps, improved accuracy, and fewer types of medicines

Inactive Publication Date: 2012-05-02
HUANGSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The ether used in this method is extremely volatile, is an anesthetic, and is a dangerous product. Ethyl acetate, n-butanol, glacial acetic acid, and ammonia water are all irritating and low-toxic drugs to the human body; the circular filter paper has a limited spread distance due to its diameter. It is relatively short and requires continuous secondary spectrum expansion to separate amino acids, resulting in a waste of drugs and no time advantage; the color reaction

Method used

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  • Quantitative measurement method for theanine
  • Quantitative measurement method for theanine
  • Quantitative measurement method for theanine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Cut the root system and bud leaves of the hydroponic seedlings of the poplar forest variety of the year's tea tree respectively in July, wash, dry, and grind. Weigh out 0.219g root and 0.391g bud leaf respectively, add 60ml of distilled water and extract in boiling water bath for 45min, shake and stir once every 10min during this period. After the leaching is completed, place it to cool to room temperature, filter and dilute to 100ml.

[0034] Cut the chromatography filter paper into a rectangle of suitable size, draw a horizontal line with a pencil at a distance of 1.5 cm from the bottom, draw a cross point on the horizontal line every 2.5 cm from left to right, and draw a mark at 10 cm from the bottom horizontally, Stop marking for the exhibit.

[0035] Use a micropipette to draw the sample and spot 40 μl of the sample at the intersection point of the filter paper. After each point, blow it dry with a hair dryer. The diameter of the sample point does not exceed 0.5 c...

Embodiment 2

[0051] Weighed 2.4g each of Xinyang Maojian, Qimen black tea and Anxi Tieguanyin tea, and the masses after drying were 2.183g, 2.237g and 2.208g respectively. Grind, add 100ml of distilled water and extract in a boiling water bath for 45 minutes, shaking and stirring once every 10 minutes during this period. After the leaching is completed, place it to cool to room temperature, filter and dilute to 100ml.

[0052] Cut the chromatographic filter paper into a rectangle of suitable size, draw a horizontal line with a pencil at 1.5cm from the bottom, and draw a cross point on the horizontal line every 2.5cm from left to right; draw a mark at 10cm from the bottom horizontally , to mark the stop of the exhibition layer.

[0053] Take the sample with a micropipette and spot 20 μl of the sample at the intersection point of the filter paper. After each point, blow it dry with a hair dryer. The diameter of the sample point does not exceed 0.5 cm.

[0054] Distilled water of the same v...

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Abstract

The invention discloses a quantitative measurement method for theanine. The method comprises the following steps of: leaching a tea sample by using water, spotting a theanine standard solution, a blank solution and a tea leaching liquor on rectangular filter paper respectively, taking an ethanol-water-ninhydrin mixed solution as a developing agent, performing vertical chromatography, drying, eluting by using an ethanol solution, and measuring the content of the theanine by comparing color at the wavelength of 570nm by using a spectrophotometer. The tea sample is leached by water and the ethanol solution is used for development, so that the stimulation of toxic substances to operators and pollution of the toxic substances on environment are avoided; ninhydrin is mixed into the developing agent, so that operating steps are reduced, and the quantification of development reaction and uniform color spots are ensured; the blank solution and the theanine working solution are spotted and subjected to chromatography, and a standard curve is made, so that a control, a standard substance and a sample are subjected to the same treatment, errors are reduced, and the measurement accuracy is improved; and all measurement steps are simple, the types of related medicines are a few, and the method is economic and environment-friendly.

Description

technical field [0001] The invention belongs to the field of biochemistry, in particular to a method for measuring the content of theanine with a spectrophotometer after chromatography on plant sample paper. [0002] Background technique [0003] L-theanine, also known as glutamyl ethylamine, English name L-Theanine, Cas number: 3081-61-6, molecular formula: C 7 h 14 N 2 o 3 , is an amide compound, white or off-white powder, melting point 214-215 degrees, easily soluble in water, with caramel aroma and fresh taste similar to monosodium glutamate. [0004] L-theanine only exists in some plants of the genus Camellia and a kind of fungus in nature, accounting for between 1% and 2% of the dry weight of tea leaves, accounting for more than 50% of the total free amino acids, and is the main substance that constitutes the flavor of tea One of them is also widely used in the food industry. Modern medicine has confirmed that theanine has many health care effects on the human bo...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/28
Inventor 马雪泷房江育邹鹏飞王荡强曹顺利
Owner HUANGSHAN UNIV
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