Extraction method of Cinnamomum camphora seed oil by ultrasound-assisted compound enzyme

An extraction method and technology for camphor seeds are applied in the field of ultrasonic-assisted compound enzyme extraction of camphor seed oil, which can solve the problems of long extraction process, many equipment, quality impact and the like.

Inactive Publication Date: 2012-05-16
SHAANXI UNIV OF TECH
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  • Application Information

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Problems solved by technology

Vegetable oils and fats are traditionally extracted by pressing, or by solvent extraction and steam distillation. The extraction proc

Method used

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Embodiment Construction

[0004] Below in conjunction with example the present invention is described in further detail.

[0005] Ultrasound-assisted compound enzyme extraction of camphor seed oil specific implementation: camphor seed→peeling→drying→crushing→adding buffer→adding compound enzyme→adding petroleum ether→ultrasonic treatment→oil separation→petroleum ether recovery→vacuum drying→ Camphor Seed Oil.

[0006] (1) Pretreatment: camphor seeds remove moldy seeds and other impurity components, remove the exocarp after cleaning, and treat at a temperature of 40-45°C for 36-48 hours to reduce the moisture content of camphor seeds to about 10%, and then The pulverizer is crushed and passed through a 60-80 mesh sieve for later use;

[0007] (2) Buffer preparation: according to the action conditions of the compound enzyme (cellulase+amylase), prepare a buffer solution of pH 4.5;

[0008] (3) Enzymolysis: Add a certain amount of compound enzyme (cellulase + amylase) solution to the buffer solution to ...

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Abstract

The invention discloses an extraction method of Cinnamomum camphora seed oil by ultrasound-assisted compound enzyme. The technical scheme comprises steps of: drying Cinnamomum camphora seeds, crushing, adding a buffer, adding compound enzyme, adding petroleum ether, carrying out ultrasonic treatment, separating grease, recovering petroleum ether, carrying out vacuum drying, and acquiring the Cinnamomum camphora seed oil. By the utilization of ultrasound cavitation, high-frequency oscillation, emulsification and diffusion effects, plant cell walls can be broken so as to accelerate diffusion and releasing of grease components and make the grease components fully contact with a solvent for grease extraction. In addition, by the ultrasonic treatment, enzyme molecular conformation may be changed and catalytic activity of compound enzyme is raised. Therefore, the extraction effect of the Cinnamomum camphora seed oil is enhanced. By the adoption of the method provided by the invention, the recovery rate of the Cinnamomum camphora seed oil can reach 36%. The invention can provide a Cinnamomum camphora seed oil raw material for relative industries.

Description

technical field [0001] The invention relates to a method for extracting camphor seed oil with ultrasonic-assisted compound enzymes. Background technique [0002] Cinnamomum camphora (Cinnamomum camphora), also known as camphor tree, camphor, belongs to Lauraceae, camphor, grows in warm temperate and subtropical regions of Asia and Oceania. Camphor contains camphor oil and camphor from root to leaf, and its seeds can be used to extract oil, which is a good raw material for making soap. Camphor tree is an excellent tree species for refining natural spices because of its special aroma and volatile oil. Studies have found that camphor seed kernels are rich in oil, and its oil content is as high as more than 40%. The fatty acid composition in oil is mainly C10 and C12 series fatty acids, which can reach about 90%. The camphor seed oil whose main component is capric acid (C10 series) can be synthesized into glycerol carbonate, which can be used to treat fat metabolism disorders ...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/10C11B1/04
Inventor 耿敬章
Owner SHAANXI UNIV OF TECH
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