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Culture medium for producing vitamin B12 by fermenting pseudomonas denitrificans and fermentation method thereof

A technology of Pseudomonas denitrification and fermentation medium, which is applied in the medium and fermentation field of Pseudomonas denitrification to produce vitamin B12, which can solve the problems of large fluctuations in product quality, restrictions on industrial production, and large differences in the quality of beet molasses to achieve the effects of reducing wastewater discharge, saving the amount of raw and auxiliary materials, and reducing overall costs

Active Publication Date: 2012-05-16
NINGXIA KINGVIT PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the influence of artificial planting and harvesting seasons, the product quality of beet molasses fluctuates greatly all the time.
The seasonality of beet molasses production is also particularly strong. The production season of beet molasses is from October to March of the following year. The supply is relatively sufficient, the molasses is fresh and of good quality, and the production of beet molasses in the remaining 6 months The output is obviously insufficient, which seriously restricts the vitamin B 12 industrial production; and the quality of beet molasses varies greatly due to the different origins of beet 12 The fermentation unit has a greater impact; in addition, the storage and transportation costs of molasses are also relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation process of the first-level seed culture medium: at 1m 3 Add artificial molasses (including 2.0kg of maltose, 7g of thiamine, 7g of riboflavin, 7g of biotin) into the first-level seed pot 、 Corn steep liquor 14L, magnesium oxide 18g, 5,6-dimethylbenzimidazole 18g, diammonium phosphate 40g, zinc sulfate 4g, calcium carbonate 20g, betaine 100g. Sterilize after the ingredients are completed. Sterilization conditions: pressure 0.10~0.14MPa; temperature 120~122℃; sterilization time 31 minutes, cooling and maintaining pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, waiting for inoculation.

[0027] Preparation process of secondary seed culture medium: at 10m 3 Add artificial molasses (26kg of maltose, 80g of thiamine, 80g of riboflavin, 80g of biotin), 200L of corn syrup, 3kg of betaine, 200g of magnesium oxide, 400g of diammonium hydrogen phosphate, 50g of zinc sulfate into the secondary seed tank. 800g of calcium carbonate, 700g of 5,6-dime...

Embodiment 2

[0035] The preparation process of the first-level seed culture medium: at 1m 3 Add artificial molasses (including 2.1kg of maltose, 7.5g of thiamine, 7.5g of riboflavin, 7.5g of biotin) 、 Corn steep liquor 15L, magnesium oxide 20g, 5,6-dimethylbenzimidazole 20g, diammonium hydrogen phosphate 42g, zinc sulfate 4.5g, calcium carbonate 25g, betaine 105g. Sterilize after the ingredients are completed. Sterilization conditions: pressure 0.10~0.14MPa; temperature 120~122℃; sterilization time 31 minutes, cooling and maintaining pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, waiting for inoculation.

[0036] Preparation process of secondary seed culture medium: at 10m 3 Add artificial molasses (including 26.5kg of maltose, 85g of thiamine, 85g of riboflavin, 85g of biotin), 210L of corn steep liquor, 3.1kg of betaine, 250g of magnesium oxide, 450g of diammonium hydrogen phosphate, and zinc sulfate into the secondary seed tank 55g, 850g calcium carbonate, 750g 5,6-d...

Embodiment 3

[0044] The preparation process of the first-level seed culture medium: at 1m 3 Add artificial molasses (including 2.2kg of maltose, 8g of thiamine, 8g of riboflavin, 8g of biotin) 、 Corn steep liquor 16L, magnesium oxide 22g, 5,6-dimethylbenzimidazole 22g, diammonium hydrogen phosphate 45g, zinc sulfate 5g, calcium carbonate 30g, betaine 110g. Sterilize after the ingredients are completed. Sterilization conditions: pressure 0.10~0.14MPa; temperature 120~122℃; sterilization time 31 minutes, cooling and maintaining pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, waiting for inoculation.

[0045] Preparation process of secondary seed culture medium: at 10m 3 Add artificial molasses (including 27kg of maltose, 90g of thiamine, 90g of riboflavin, 90g of biotin), 220L of corn steep liquor, 3.2kg of betaine, 300g of magnesium oxide, 500g of diammonium hydrogen phosphate, and 60g of zinc sulfate into the secondary seed tank , 900g calcium carbonate, 800g 5,6-dimethy...

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Abstract

The invention relates to a culture medium for producing vitamin B12 by fermenting pseudomonas denitrificans and a fermentation method thereof, and a method for producing vitamin B12 by the same. The culture medium comprises a primary seed culture medium, a secondary seed culture medium and a fermentation culture medium all of which contain artificial molasses composed of maltose, thiamine, riboflavin and biotin. By substituting the artificial molasses composed of maltose, thiamine, riboflavin and biotin for beet molasses and optimizing the formula of the culture medium, the invention solves the problem that the fluctuation range of fermentation titer is large because the quality of beet molasses is unstable, and acquires a method for stably and effectively producing vitamin B12. The invention saves the doses of raw and auxiliary materials, lowers the comprehensive cost and reduces the waste water discharge.

Description

technical field [0001] The invention belongs to the technical field of fermentation, in particular to a kind of Pseudomonas denitrifica fermented to produce vitamin B 12 Culture medium and fermentation method. Background technique [0002] Vitamin B 12 It is a cobalamin compound, mainly used for the treatment of pernicious anemia, and also used in combination with folic acid for the treatment of various megaloblastic anemia, anemia and steatorrhea caused by antifolate drugs. It is also used for the treatment of nervous system diseases (such as neuritis, nerve atrophy). [0003] Currently commercialized vitamin B at home and abroad 12 Almost all are produced by microbial fermentation. Among them, vitamin B is produced by fermentation of Pseudomonas denitrification bacteria 12 The raw and auxiliary materials for production are mainly made of carbon and nitrogen sources such as beet molasses and sucrose and a small amount of inorganic salts for liquid submerged fermentatio...

Claims

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Application Information

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IPC IPC(8): C12P19/42C12R1/38
Inventor 任勇冷晓红王友善奇乃董媛
Owner NINGXIA KINGVIT PHARMA
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