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Process for production of organic acids

A technology of tartaric acid and glycolic acid, which is applied in chemical instruments and methods, organic compound/hydride/coordination complex catalysts, physical/chemical process catalysts, etc., can solve the problems of low yield and cannot be prepared with glycolic acid, etc. achieve high practicality

Inactive Publication Date: 2012-05-23
HYPHAGENESIS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Like this, because the yield of this preparation method is very low, this preparation method cannot be used in the preparation of the glycolic acid on the industry

Method used

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  • Process for production of organic acids
  • Process for production of organic acids
  • Process for production of organic acids

Examples

Experimental program
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Effect test

Embodiment 1

[0052] [Acquisition of 2-keto-D-gluconic acid non-producing bacteria and 5-keto-D-gluconic acid non-consuming mutant strains]

[0053] In order to prepare a 2-keto-D-gluconic acid non-producing strain derived from the Gluconobacter oxydans NBRC 3172 strain, an NTG mutation treatment was performed on the NBRC 3172 strain, and screening of 2-keto-D-gluconic acid non-producing bacteria was attempted. Specifically, it was carried out by the following method.

[0054] Oxidation cells grown on MA agar medium [2.5% mannitol, 0.5% yeast extract (manufactured by Difuco Laboratori Co., Ltd.), 0.2% peptone (manufactured by Diifco Laboratori Co., Ltd.), 1.5% agar (no pH adjustment)] Gluconobacter NBRC 3172 strain was inoculated with 1 platinum circle into a 300-mL Erlenmeyer flask containing 30 mL of MA medium sterilized in an autoclave at 121°C for 20 minutes, and cultured with shaking at 220 rpm per minute at 30°C After 19 hours, cells were aseptically collected from the culture soluti...

Embodiment 2

[0058] [(Preparation of Tartaric Acid and Glycolic Acid from Glucose (Addition of Transition Metal Catalyst))]

[0059] The Gluconobacter oxidans TADK-267 strain obtained in the above Example 1 was inoculated into 5 mL of MB medium [2.5% mannitol, 0.5% yeast extract (Difuco Laboratori Co., Ltd. ) and 0.3% peptone (manufactured by Difuco Laboratori Co., Ltd.)], and cultured at 28° C. for 17 hours with reciprocating shaking at 250 rpm. The culture solutions in the three test tubes were combined as the seed culture solution. This culture is carried out as follows. In a 1L fermenter (manufactured by Eibul Corporation), add 6% glucose, 0.09% ammonium chloride, 0.06% potassium dihydrogen phosphate, 0.18% corn steep liquor dry powder (manufactured by Sigma), 0.1% Yeast extract (manufactured by Difuko Laboratori Co., Ltd.), 0.015% magnesium sulfate heptahydrate, 0.0029% manganese sulfate pentahydrate, 0.12% calcium chloride dihydrate, and 0.1% Actocol (manufactured by Takeda Chemical...

Embodiment 3

[0061] [Preparation of tartaric and glycolic acids from glucose (adding palladium on carbon)]

[0062]After cultivating Gluconobacter oxydans TADK-267 in a fermenter for 50 hours in the same manner as in Example 2 above, glucose was completely consumed and 2.8 g / L of gluconic acid remained, but 67.4 g / L of 5-keto- D-gluconic acid. After adding 6M potassium hydroxide solution to the culture solution to raise the pH of the culture solution to 9.6, 10 g of palladium activated carbon (10%, manufactured by Wako Pure Chemical Industries, Ltd.) was added, and then potassium hydroxide solution was added to maintain the pH at At the same time as above 9.6, continue aeration and stirring until after the 144th hour, a reaction solution containing 31.9 g / L of tartaric acid and 15.9 g / L of glycolic acid is obtained. If considering the change of the liquid volume caused by evaporation and adding potassium hydroxide solution, and the liquid volume reduction caused by sampling in the middle ...

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Abstract

Disclosed is a process for producing L-tartaric acid or a salt thereof and / or glycolic acid or a salt thereof from glucose, which comprises the following steps (A) to (C): (A) culturing a microorganism capable of producing 5-keto-D-gluconic acid from glucose in a glucose-containing culture medium in the presence of an alkali capable of producing a water-soluble salt of 5-keto-D-gluconic acid, thereby producing a culture containing a water-soluble salt of 5-keto-D-gluconic acid; (B) adjusting the pH value of the culture containing the water-soluble salt of 5-keto-D-gluconic acid, which has been produced in step (A), to 7 to 12 and retaining the culture at that pH value, thereby producing a reaction solution in which the water-soluble salt of 5-keto-D-gluconic acid is converted into L-tartaric acid or a salt thereof and / or glycolic acid or a salt thereof; and (C) collecting L-tartaric acid or the salt thereof and / or glycolic acid or the salt thereof from the reaction solution which has been produced in step (B).

Description

technical field [0001] The present invention relates to a method for producing L-tartaric acid or a salt thereof, and / or glycolic acid or a salt thereof from glucose. Background technique [0002] L-tartaric acid or its salts are used as sour agents and pH regulators in the food field, as cosmetics, dyeing, lotion, and plating in the industrial field, and as pharmaceutical raw materials in the pharmaceutical field, and are widely used in the industry. Useful substances. In addition, when L-tartaric acid is produced from glucose, glycolic acid produced simultaneously with L-tartaric acid is useful as a metal detergent, a plating additive, a cosmetic additive, a raw material for hard biodegradable polymers, and the like. [0003] Various methods are known for producing L-tartaric acid, but it is currently produced from winemaking residues and petroleum-derived intermediate maleic anhydride. Furthermore, glycolic acid is produced by petrochemical methods. On the other hand, ...

Claims

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Application Information

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IPC IPC(8): C12P7/42B01J31/28B01J31/30B01J31/32C12P7/50
CPCC12P7/46C12P7/42
Inventor 星野达雄田副正明小林节子清水明子
Owner HYPHAGENESIS
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