Method of preparing taxifolin monomer from engelhardtia leaf and application

A technology of taxifolin and wolfberry leaves, applied in the field of medicine, can solve the problems of large environmental pollution, damage, severe reaction conditions, etc., and achieve the effects of strong reaction specificity, overcoming large damage, and simplifying operation steps.

Inactive Publication Date: 2012-05-30
TIANJIN INSTITUTE OF PHARMA RESEARCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the larch resource itself is relatively scarce, and the content of taxifolin in the larch is also low, generally less than 2%. It is used to produce taxifolin, which not only causes waste and damage to the already scarce larch resources, but also has a relatively low yield. Low
The hydrolysis in the above two inventions all adopts acid hydrolysis, the reaction conditions are severe, the product is easily destroyed, and the complete aglycon is often no

Method used

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  • Method of preparing taxifolin monomer from engelhardtia leaf and application
  • Method of preparing taxifolin monomer from engelhardtia leaf and application
  • Method of preparing taxifolin monomer from engelhardtia leaf and application

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Grind the dried Huangqi leaves, put them in a multifunctional extraction tank, add 10 times the amount of 70% ethanol for reflux extraction for 2 hours for the first time, add 8 times the amount of 70% ethanol for reflux extraction for 2 hours for the second and third times, and combine the extraction liquid, concentrated under reduced pressure to obtain a paste. Add 100ml of disodium hydrogen phosphate-citric acid buffer solution with pH=5.0 per gram of the obtained paste, add β-glucosidase according to enzyme / paste=0.05, react at 50°C for 48h, add 4 times the volume of ethanol to terminate The reaction was centrifuged to remove the precipitate. The supernatant was concentrated until it had no alcohol smell, and was adsorbed by FL-1 macroporous resin, eluted with water and 10% ethanol respectively until the color of the effluent was very light, and impurities were removed, and then eluted with 40% ethanol until the effluent was free of color. The eluents were combine...

Embodiment 2

[0049] Grind the dried Huangqi leaves, add 5 times the amount of 90% ethanol for ultrasonic extraction for 1 h, filter out the medicinal liquid, and then use 60% ethanol for ultrasonic extraction for 1 h, combine the two filtrates, and concentrate under reduced pressure to extract. Add 50ml of disodium hydrogen phosphate-citric acid buffer solution with pH=5.0 to each gram of the obtained paste, add β-glucosidase according to enzyme / paste=0.05, react at 50°C for 48h, add 4 times the volume of ethanol to terminate The reaction was centrifuged to remove the precipitate. The supernatant was concentrated until it had no alcohol smell, and was adsorbed by FL-1 macroporous resin, eluted with water and 10% ethanol respectively until the color of the effluent was very light, and impurities were removed, and then eluted with 40% ethanol until the effluent was free of color. The eluents were combined, and the ethanol was recovered under reduced pressure until a precipitate was precipit...

Embodiment 3

[0051] Grind the dried Huangqi leaves and place them in a multi-functional extraction tank, add 10 times the amount of 90% ethanol for reflux extraction for 2 hours for the first time, add 8 times the amount of 60% ethanol for reflux extraction for 2 hours for the second and third times, and combine the extraction liquid, concentrated under reduced pressure to obtain a paste. Add 50ml of acetic acid-sodium acetate buffer solution with pH=4.8 to each gram of the obtained paste, add β-glucosidase according to enzyme / paste=1, react at 50°C for 4h, add 4 times the volume of ethanol to terminate the reaction, and remove by centrifugation precipitation. The supernatant was concentrated until it had no alcohol smell, was adsorbed by FL-1, eluted with water and 10% ethanol respectively until the color of the effluent was very light, and impurities were removed, and then eluted with 40% ethanol until the effluent was colorless. The eluents were combined, and the ethanol was recovered ...

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Abstract

The invention discloses a method of preparing a taxifolin monomer from engelhardtia leaves and an application, and the method comprises the following steps: crushing or cutting dried engelhardtia leaves into segments, adding ethanol with a certain concentration, performing extraction, concentrating the obtained extract liquid to paste; adding a buffer with a pH of 3-6, adding a proper amount of enzymes for enzymatic hydrolysis at a certain temperature, performing separation, enrichment, purification, and recrystallization treatment of the enzymatic hydrolysate to obtain a taxifolin monomer with purity of above 99%. The method of the invention is characterized in that enzymatic hydrolysis of the engelhardtia leaf extract is directly performed to obtain the taxifolin monomer with high purity, and the method of the invention has the advantages of strong reaction specificity, high conversion rate, simple and practical process, less pollution, and suitability for large-scale industrial production, and the like. The disadvantages of great destructive effect on aglycone, poor specificity, low conversion rate, high equipment corrosivity, severe pollution, tedious process and the like in the prior art are overcome. The taxifolin monomer prepared in the invention is applicable to the prevention and treatment of fatty liver.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and relates to a method for enzymatically hydrolyzing an extract of Huangqi leaves by adopting enzymolysis technology, separating and preparing taxifolin monomer from the enzymolysis product, and using it for preventing and treating fatty liver. Background technique: [0002] Huangqi Ye is the dry leaf of Engeihardtia roxburghiana Wall, a plant of the family Juglandaceae, which has the effect of clearing heat, detoxifying and relieving pain. It is often used as a health-care tea drink in the Guangdong and Guangxi areas. It has certain health functions such as promoting blood circulation, reducing blood fat, lowering blood sugar, and improving immunity, as well as various physiological activities such as anti-oxidation, obesity suppression, anti-allergy, and cancer prevention. [0003] Taxifolin, also known as dihydroquercetin and taxifolin, its chemical name is - pentahydroxydihydroflavone, the...

Claims

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Application Information

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IPC IPC(8): C12P17/06A61K31/352A61P1/16A61P3/06
Inventor 侯文彬张新鑫单淇徐旭高贤周福军
Owner TIANJIN INSTITUTE OF PHARMA RESEARCH
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