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Method for producing sophorolipid through fermentation of lignocellulose material

A technology of lignocellulose and microbial fermentation biosurfactant, which is applied in the field of using lignocellulose material to ferment and produce microbial fermentation biosurfactant by using a Wick yeast variant, and achieves the effects of low price, simple process and reduced production cost.

Inactive Publication Date: 2013-05-29
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the fact that there is no report on the production of sophorolipids by fermentation of biomass resources at present, the problem to be solved in the present invention is to provide a method for the production of sophorolipids by fermentation of lignocellulosic materials, that is, using the hydrolyzate of biomass resources and the hydrolyzate produced by the hydrolyzate Method for producing single-cell oil and producing sophorolipids by fermenting Pseudomonas spp.

Method used

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  • Method for producing sophorolipid through fermentation of lignocellulose material
  • Method for producing sophorolipid through fermentation of lignocellulose material
  • Method for producing sophorolipid through fermentation of lignocellulose material

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Effect test

Embodiment 1

[0027] (1) Preparation of cellulase solution: inoculate Penicillium decumbens JU-A10 in a regular amount in the enzyme-producing medium, shake culture at 250 rpm at 30°C for 96 h to produce enzyme, centrifuge at 6000 rpm for 30 min, and collect The supernatant is the cellulase crude enzyme solution;

[0028] The formula of the above-mentioned enzyme production medium is: 20g / l xylose residue, 30g / l bran, 6g / l microcrystalline cellulose, 5g / l bean cake powder, 2g / l ammonium sulfate, 1g / l urea, 2g / l nitric acid Sodium, 3g / l potassium dihydrogen phosphate, 0.5g / l magnesium sulfate, 3ml / l Tween 80;

[0029] (2) Preparation of saccharification solution: take delignified xylose residue, add cellulase solution with 25 FPA per gram of dry substrate, and saccharify at 45°C for 72 hours; collect the saccharification solution and put the residual cellulose in a water bath at 80°C for 30 minutes. Inactivate the enzyme, then centrifuge the saccharification solution at 8000 rpm for 10 minu...

Embodiment 2

[0036] (1) Preparation of cellulase solution: Inoculate Penicillium decumbens in an enzyme-producing medium in a conventional amount, culture at 30°C and shake at 250rpm for 84h to produce enzyme, centrifuge at 6000rpm for 30min, and collect the supernatant as cellulose Enzyme crude enzyme solution;

[0037] The formula of the above-mentioned enzyme production medium is: 20g / l xylose residue, 30g / l bran, 6g / l microcrystalline cellulose, 5g / l bean cake powder, 2g / l ammonium sulfate, 1g / l urea, 2g / l nitric acid Sodium, 3g / l potassium dihydrogen phosphate, 0.5g / l magnesium sulfate, 3ml / l Tween 80;

[0038] (2) Preparation of saccharification solution: take delignified xylose residue, add cellulase solution with 30 FPA per gram of dry substrate, and saccharify at 45°C for 72 hours; collect the saccharification solution and place it in a water bath at 80°C for 30 minutes to remove residual cellulose Inactivate the enzyme, then centrifuge the saccharification solution at 9000 rpm f...

Embodiment 3

[0045] (1) Preparation of saccharification solution: take delignified xylose residue, add commercial cellulase solution (from Qingdao Kangdien Biotechnology Co., Ltd.) with 25 FPA per gram of dry substrate, and the buffer system is pH4.8 The 0.05M HAC-NaAc buffer solution was saccharified at 45°C for 72 hours; the saccharification solution was collected and placed in a water bath at 80°C for 30 minutes to inactivate residual cellulase, and then the saccharification solution was centrifuged at 8000rpm for 10 minutes to obtain a clear saccharification solution;

[0046] (2) Detoxification treatment of the saccharified liquid: heating the saccharified liquid in a water bath to 80° C., adding 1.0% activated carbon by mass percentage, stirring for 30 minutes, and then vacuum filtering to remove the activated carbon to obtain a detoxified saccharified liquid;

[0047] (3) Preparation of single-cell oil from saccharified liquid: prepare oil-producing saccharified liquid fermentation m...

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Abstract

The invention discloses a method for producing sophorolipid through fermentation of a lignocellulose material. The method comprises the steps of: carrying out shaking table oscillation fermentation on hydrolysate of delignified xylose residue, xylose residue, corn straw, corn cob powder, grass meal and other biomass resources and single cell oil generated by the hydrolysate in the presence of Wickerhamiella domercqiae variant at 30+ / -2 DEG C and 150-300 rpm to obtain fermentation liquid containing sophorolipid, and carrying out rough extraction on the fermentation liquid to obtain sophorolipid. According to the method disclosed by the invention, the lignocellulose saccharification liquid is used to replace the sugar required for producing the single cell oil and sophorolipid, and the single cell oil is used to replace a hydrophobic substrate required for producing sophorolipid, thus obtained sophorolipid is easy to separate and high in yield; the method has the characteristics of richand inexpensive raw materials, simple and convenient process and low cost; a great deal of biomass resources are utilized, and agricultural wastes are treated; and the method has important practical significance and important economic value in improving energy insufficiency condition in China, guaranteeing sustainable energy supply in China and alleviating energy crisis.

Description

technical field [0001] The invention relates to a method for fermenting and producing sophorolipids, in particular to a method for fermenting and producing sophorolipids by using lignocellulosic materials with Wickerhamiella domercqiae var. sophorolipid. Background technique [0002] Sophorolipid is an important biosurfactant, which is mainly obtained through microbial fermentation. Generally, sophorolipid-producing bacteria can secrete sophorolipids in large quantities when hydrophobic carbon sources and hydrophilic carbon sources exist at the same time. Compared with chemically synthesized surfactants, sophorolipids not only have excellent surface properties, stable emulsifying ability, and obvious foaming effect, but also have good biodegradability, biocompatibility, etc., and have antibacterial and antitumor properties. , antiviral and other biological activities. These advantages make sophorolipids have great prospects for commercial application. [0003] As a biosur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/12C12R1/645C12R1/845C12R1/80
Inventor 宋欣马晓静
Owner SHANDONG UNIV
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